The Effect of Exposure to 900 MHz Radiofrequency Radiation on Micronucleus Formation and Cell Cycle Kinetics in Immature Rat Lymphocytes
[1]
El Idrissi Sidi Brahim Salem, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco; Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[2]
Boussaber El Arbi, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
[3]
El Goumi Younes, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
[4]
Talbi Hayat, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
[5]
Choukri Abdelmajid, Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[6]
Hillali Abderraouf, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
Due to the expansion of cellular mobile (GSM) in recent decades, many voices are raised against the abusive use of these objects by children or teenagers. The early life exposure (fetus, babies and child) to radiofrequency (RF) radiation may increase the risk of developing diseases in later life. The aim of our study was to evaluate whether whole-body exposure to non-thermal radiofrequency (RF) radiation from cellular phones could induce cytogenetic effects on peripheral blood lymphocytes of immature rats exposed to radiofrequency radiation. Twelve (20) Wistar immature rats were used for the study and randomly divided into 4 groups according to time of exposure, oh (control), 1h, 2h and 3h daily 7 days a week for up 7 weeks to 9000 MHz at an average special absorption rate (SAR) of 0.873-0.352 W/kg, all rats were visually checked daily and body weight and length were measured weekly. After the period of exposure, blood samples were collected from all groups and peripheral blood cultures were performed using standard laboratory methods for the extent of genotoxicity, assessed by the cytokinesis-block micronucleus assay. The results indicated that significant increase of micronucleated (MN) cells in the lymphocytes among the exposed groups compared with the control. The proliferation index (PI), is significantly decreased in all exposed groups compared with the control, however body weight and length gains were insignificantly changed in all groups exposed comparing to unexposed rats. This study shows that the exposure to 900 MHz radiofrequency radiation from cell phones may induce cytogenetic alterations in immature rat lymphocytes and this effect is less remarkable with increasing time of exposure.
Immature Rat Lymphocytes, Radiofrequency, Micronucleus Test, Proliferation Index, Body Weight, Length Gains
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