Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News
The Effect of Exposure to 900 MHz Radiofrequency Radiation on Micronucleus Formation and Cell Cycle Kinetics in Immature Rat Lymphocytes
Current Issue
Volume 4, 2017
Issue 1 (January)
Pages: 1-8   |   Vol. 4, No. 1, January 2017   |   Follow on         
Paper in PDF Downloads: 38   Since Jun. 15, 2017 Views: 1250   Since Jun. 15, 2017
Authors
[1]
El Idrissi Sidi Brahim Salem, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco; Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[2]
Boussaber El Arbi, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
[3]
El Goumi Younes, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
[4]
Talbi Hayat, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
[5]
Choukri Abdelmajid, Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[6]
Hillali Abderraouf, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1er, Settat Km 3, B. P: 577. Morocco.
Abstract
Due to the expansion of cellular mobile (GSM) in recent decades, many voices are raised against the abusive use of these objects by children or teenagers. The early life exposure (fetus, babies and child) to radiofrequency (RF) radiation may increase the risk of developing diseases in later life. The aim of our study was to evaluate whether whole-body exposure to non-thermal radiofrequency (RF) radiation from cellular phones could induce cytogenetic effects on peripheral blood lymphocytes of immature rats exposed to radiofrequency radiation. Twelve (20) Wistar immature rats were used for the study and randomly divided into 4 groups according to time of exposure, oh (control), 1h, 2h and 3h daily 7 days a week for up 7 weeks to 9000 MHz at an average special absorption rate (SAR) of 0.873-0.352 W/kg, all rats were visually checked daily and body weight and length were measured weekly. After the period of exposure, blood samples were collected from all groups and peripheral blood cultures were performed using standard laboratory methods for the extent of genotoxicity, assessed by the cytokinesis-block micronucleus assay. The results indicated that significant increase of micronucleated (MN) cells in the lymphocytes among the exposed groups compared with the control. The proliferation index (PI), is significantly decreased in all exposed groups compared with the control, however body weight and length gains were insignificantly changed in all groups exposed comparing to unexposed rats. This study shows that the exposure to 900 MHz radiofrequency radiation from cell phones may induce cytogenetic alterations in immature rat lymphocytes and this effect is less remarkable with increasing time of exposure.
Keywords
Immature Rat Lymphocytes, Radiofrequency, Micronucleus Test, Proliferation Index, Body Weight, Length Gains
Reference
[1]
Grigoriev IuG., Radiats Biol Radioecol. 2005 Jul-Aug; 45(4):442-50. [The electromagnetic fields of cellular phones and the health of children and of teenagers (the situation requiring to take an urgent measure)].
[2]
Schuz J., Bioelectromagnetics. 2005;Suppl 7:S45-50. Mobile phone use and exposures in children.
[3]
Wiart J, Hadjem A, Wong MF, Bloch I. Analysis of RF expo-sure in the head tissues of children and adults. Phys Med Biol2008;53(13):3681–95.
[4]
Kabuto M et al (17 scientists), Int J Cancer. 2006 Feb 22; Childhood leukemia and magnetic fields in Japan: A case-control study of childhood leukemia and residential power-frequency magnetic fields in Japan.
[5]
Hardell L, K H Mild, M Carlberg and F Söderqvist,(2006). Tumour risk associated with use of cellular telephones or cordless desktop telephones. World J Surg Oncol Oct 11;4(1):74.
[6]
Vijayalaxmi, L. B. Sasser, J. E. Morris, B. W. Wilson, L. E. Anderson, (2003). Genotoxic potential of 1.6 GHz wireless communication signal: in vivo two-year bioassay, Radiat. Res. 159 (4) 558–564.
[7]
Fenech, M. 1993. The cytokinesis – block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in a human population. Mutat. Res., 285: 35-44.
[8]
Titenko-Holland, N., Windhan, G., Kolachana, P., Reinish, F., Paravatham, S., Osorio, A. M. and Smith, M. T, (1997). Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mut. Res., 388: 85-95.
[9]
Sambucci, M., F. Laudisi, et al. (2011). "Early life exposure to 2.45GHz WiFi-like signals:Effects on development and maturation of the immune system." Progress in Biophysics and Molecular Biology 107(3): 393-398.
[10]
Ait-Aissa, S., Billaudel, B., Poulletier De Gannes, F., Hurtier, A., Haro, E., Taxile, M., Ruffié, G., Athane, A.,Veyret, B., Lagroye, I., 2010. In situ detection of gliosis and apoptosis in the brains of young rats exposed in utero to a Wi-Fi signal. Comptes Rendus Physique 11, 592-601.
[11]
Orendácová, J., Raceková, E., Orendác, M., Martoncíková, M., Saganová, K., Lievajová, K., Abdiová, H., Labun, J.,Gálik, J., 2009. Immunohistochemical study of postnatal neurogenesis after whole-body exposure to electromagnetic fields: evaluation of age- and dose-related changes in rats. Cell Mol. Neurobiol. 29(6-7), 981-990.
[12]
Fadel RA et al., Anthropol Anz. 2006 Jun; 64(2):211-26. Growth assessment of children exposed to low frequency electromagnetic fields at the Abu Sultan area in Ismailia (Egypt).
[13]
Hugo W. Ruediger, (2009). Genotoxic effects of radiofrequency electromagnetic fields Pathophysiology16 89–102.
[14]
Sekeroglu Vedat, Aysegul Akar, Zulal Atlı Sekeroglu, (2012). Cytotoxic and genotoxic effects of high-frequency electromagnetic fields (GSM 1800 MHz) on immature and mature rats Ecotoxicology and Environmental Safety 80 140–144.
[15]
Aydin B, Akar A. Effects of a 900-MHz electromagnetic field on oxidative stress parameters in rat lymphoid organs, polymorphonuclear leukocytes and plasma. Arch Med Res. 42(4):261-267, 2011.
[16]
Sun, L. X., Yao, K., Jiang, H., He, J. L., Lu, D. Q., Wang, K. J., Li, H. W., 2006. DNA damage and repair induced by acute exposure of microwave from mobile phone on cultured human lens epithelial cells. Zhonghua Yan. Ke. Za. Zhi 42, 1084–1088.
[17]
Diem E, Schwarz C, Adlkofer F, Jahn O, et Rüdiger H,( 2005). Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutât Res;583:178-183.
[18]
Campisi, A., M. Gulino, et al. (2010). "Reactive oxygen species levels and DNA fragmentation on astrocytes in primary culture after acute exposure to low intensity microwave electromagnetic field." Neurosci Lett 473(1): 52-55.
[19]
Komatsubara Y., Hirose H., Sakurai T. et al. (2005). Effect of high-frequency electromagnetic fields with a wide range of SARs on chromosomal aberrations in murine m5S cells. Mutat Res.; 587 (1-2):114-9.
[20]
Zeni, O., Schiavoni, A., Perrotta, A., Forigo, D., Deplano, M., Scarfi, M. R., 2008. Evaluation of genotoxic effects in human leukocytes after in vitro exposure to 1950 MHz UMTS radiofrequency field. Bioelectromagnetics 29, 177–184.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
CONTACT US
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
E-mail:
LET'S GET IN TOUCH
Name
E-mail
Subject
Message
SEND MASSAGE
Copyright © 2013-, Open Science Publishers - All Rights Reserved