Background: The data for the determination of chromium concentrations in biological samples are still contradictory and for some biological objects varied within orders of magnitude. They are not fully clarified the role and participation in the metabolism in healthy subjects and in various diseases and conditions. Aim: One reason for the outstanding problems in the study of the functions of the chromium in the human organism is a need for accurate, with low detection limits, and access to a biomedical laboratory methods for the analytical determination. That’s why we set the aim to make a brief review of spectral methods for determination of chromium in human body fluids and tissues. Methods, results: Analytical methods which most commonly to apply at determining the chromium in biomaterials are the atomic spectral (AS) - Flame atomic absorption spectroscopy, Electrothermal atomic absorption spectrometry, Inductively coupled plasma optical emission spectrometry and Inductively coupled plasma mass spectrometry. They are accurate and have lower limits of detection, but not all biomedical laboratories have them. Of the molecular spectral, applicable methods with spectrophotometric indication for determination of chromium in biomaterials are the methods which used suitable chelating agents of chromium in micellar medium. They have limits of detection, comparable to AS-methods and are available to all biomedical laboratories. Conclusions: The lag in specifying the chromium concentrations in human bio-samples, related with clarifying the role of chromium in health and disease, can be overcome using both atomic spectral and spectrophotometric methods. Any study relating to the determination of chromium in human body fluids and tissues will contribute to the analytic of chromium and related the determining his biomedical research.

Cr AS-methods Body Fluids, Cr AS-methods Tissues, Cr Photometric Body Fluids, Cr Photometric Tissues