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Molecular Cloning, Expression and Regulation of Fatty Acid Desaturases 6 (FADS 6) Gene in Goose Fatty Liver
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Volume 7, 2020
Issue 1 (January)
Pages: 1-7   |   Vol. 7, No. 1, January 2020   |   Follow on         
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Rashid Habiballa Osman, Colleage of Animal Production, West Kordofan University, El Nuhud, Sudan; College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Daoqing Gong, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Long Liu, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Ahmed Kamel, College of Veterinary Science, Seuz Canal University, Seuz, Egypt.
Mojahid Abdallah Abdalhag, College of Animal Science and Technology, Yangzhou University, Yangzhou, China; College of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
Tamador Algam, College of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
Wafaa Babiker Zomrawi, Faculty of Agriculture and Natural Resources, University of Bakht Elruda, Al-Dueim, Sudan.
Fredrick Baraka Kaingu, School of Agriculture & Biological Sciences, Karatina University, Nyeri, Kenya.
In goose industry fatty liver weight is an important trait. Fatty acid desaturase is a potential therapeutic target as Fads expression and mutations are associated with liver fat. Here, we are first employed RNA-seq technology to determine the differentially expressed genes in the livers from normally-fed vs. overfed geese, followed by bioinformatics analysis and quantitative PCR validation in goose liver and primary hepatocytes cell. Based on amino acid sequence a phylogenetic tree of goose Fads6 gene had a higher identity in Duck. Data indicated that hepatic Fads6 expression was gradually increased with the time of overfeeding. Moreover, cell studies showed that Fads 6 expression was regulated by fatty liver associated factors.
Fatty Liver, Goose, Fatty Acid Desaturase-6, Overfeeding, Primary Hepatocytes
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