Molecular Cloning, Expression and Regulation of Fatty Acid Desaturases 6 (FADS 6) Gene in Goose Fatty Liver
[1]
Rashid Habiballa Osman, Colleage of Animal Production, West Kordofan University, El Nuhud, Sudan; College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
[2]
Daoqing Gong, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
[3]
Long Liu, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
[4]
Ahmed Kamel, College of Veterinary Science, Seuz Canal University, Seuz, Egypt.
[5]
Mojahid Abdallah Abdalhag, College of Animal Science and Technology, Yangzhou University, Yangzhou, China; College of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
[6]
Tamador Algam, College of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
[7]
Wafaa Babiker Zomrawi, Faculty of Agriculture and Natural Resources, University of Bakht Elruda, Al-Dueim, Sudan.
[8]
Fredrick Baraka Kaingu, School of Agriculture & Biological Sciences, Karatina University, Nyeri, Kenya.
In goose industry fatty liver weight is an important trait. Fatty acid desaturase is a potential therapeutic target as Fads expression and mutations are associated with liver fat. Here, we are first employed RNA-seq technology to determine the differentially expressed genes in the livers from normally-fed vs. overfed geese, followed by bioinformatics analysis and quantitative PCR validation in goose liver and primary hepatocytes cell. Based on amino acid sequence a phylogenetic tree of goose Fads6 gene had a higher identity in Duck. Data indicated that hepatic Fads6 expression was gradually increased with the time of overfeeding. Moreover, cell studies showed that Fads 6 expression was regulated by fatty liver associated factors.
Fatty Liver, Goose, Fatty Acid Desaturase-6, Overfeeding, Primary Hepatocytes
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