Influence Allogeneic Mesenchymal Stem Cells on the Tumour Growth Parameters and Metastatic Potential in the Transplantable Carcinoma Lung Lewis
[1]
Kladnytska Larysa V , Ukrainian education And Research Institute of Bioresourses Quality and Life Safety, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine.
[2]
Nikulina Viktoriia V , Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv, Ukraine.
[3]
Garmanchuk Liudmyla V , Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv, Ukraine.
[4]
Mazurkevych Anatoliy Y , Ukrainian education And Research Institute of Bioresourses Quality and Life Safety, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine.
[5]
Kovpak Vitaliy V , Ukrainian education And Research Institute of Bioresourses Quality and Life Safety, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine.
[6]
Nikolaienko Tetiana V , Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv, Ukraine.
[7]
Shelest Dmitro V , Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv, Ukraine.
[8]
Dzhus Olena I , Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv, Ukraine.
[9]
Skachkova Oksana V , National Cancer Institute, Kyiv, Ukraine.
[10]
Stupak Yulia A , Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv, Ukraine.
[11]
Dasyukevich Olga I , R. E. Kavetsky Institute of Experimental Pathology, Oncology, and Radiobiology, Kyiv, Ukraine.
It was investigated the effect of allogeneic mesenchymal stem cell biological characteristics of the primary tumor, metastasis in male mice C57BL/6, which transplanted cell line Lewis lung carcinoma. It was studied the effect of allogeneic MSCs on tumor weight and volume metastasis in tumor-bearing animals, on level of apoptotic cells and tumor cells distribution in cell cycle phases. The primary culture was obtained from transplantable Lewis lung carcinoma. Apoptotic level was measured by flow cytometry, tumor and metastasis volume were assessed by routine methods of experimental oncology, and level of tumor infiltration by lymphocytes was estimated after their fractionation in ficoll-triombrast gradient. We have shown that intravenous administration of allogeneic mesenchymal stem cells after 8 day transplantation of carcinoma lung Lewis leads to enhance of tumor growth in 1.5 times, tumor infiltration by lymphocytes, volume of metastasis in 3 times, and influences on biological characteristics of primary tumor cells, and it leads to an increase in aneuploidy index (in 1,3 times) and mitotic index and reduced levels of apoptotic cells.
Allogeneic MSCs, Cell Cycle Phases, Apoptosis, Tumor, Metastasis
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