[1]
Farjana Haq, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[2]
Abu Sayeed Md. Abdullah, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
This experiment was conducted to investigate the physiochemic, biochemicand immunogenic properties of P. multocida strains which were isolated from rat of poultry farm. In physiochemical properties the organisms were found to be detected as Gram - negative, non - motile, non - spore forming rod in single or pair and occasionally as chains or filaments. In biochemical tests the isolates produced catalase, oxidase, indole and ornithine decarboxylase and failed to produce urease, gelatinase, methyl red and acetone. With respect to fermentable sugars, the isolates consistently produced acid from glucose, mannitol and mannose. The immunogenic property of the organism was studied upon vaccination with prepared vaccine with the respective isolate. Primary vaccination with the prepared formalin killed fowl cholera vaccine was administered at the dose rate of 1ml containing 5×107 colony forming unit through intramuscular and subcutaneous routes in experimental birds respectively. Booster dose was given with the same dose and via same route after 7, 15 and 25 days of primary vaccination in the same group of birds. In immunogenic properties of the isolates the antibody level of pre - vaccination and post - vaccination sera was determined by passive haemagglutination test where antibody titer was highest on 21 days of post vaccination in two groups of birds and comparatively antibody titer of sera from intramuscularly vaccinated birds was higher than that of subcutaneously vaccinated birds. The experimental fowl cholera vaccine conferred 100% protection against challenge was also demonstrated. Fowl cholera vaccine prepared from rat of poultry farm was found safe and effective for the vaccination of birds against P. multocida infection.
[1]
Confer W. Immunogens of Pasteurella. Vet. Microbiol. 1993; 37: 353–68.
[2]
Chung J, Wilkie I, Boyce JD, Townsend KM, Frost AJ, Majid Ghoddusi M. Role of Capsule in the Pathogenesis of Fowl Cholera Caused by Pasteurella multocida Serogroup A. Monash Univ. Victoria. 2000.
[3]
Chowdhury KA, Amin MM, Rahman. Investigation of natural outbreak of fowl cholera. Bangladesh Vet. J. 1985; 19 (1–4):49–56.
[4]
Christensen JP & MB. Fowl Cholera. Int. der Épizooties. vol. 2000; 19 (1–4): 49–56.
[5]
Buxton A and Fraser. A haemagglutination test for the identification of serological types. Animal microbiology. Anim. Microbiol. 1977; l(1): 121–133.
[6]
F. Jabbari, A. R.; Saharee, A. A. A.; Esmaily, “Phenotypic characterisation of Pasteurella multocida obtained from poultry in Iran.,” Jurnal - Veterinar - Malaysia., vol. 15, pp. 15–19, 2003.
[7]
U. K, “Characteristics of Pasteurella multocida isolated from humans, swine and poultry in Thailand.,” Southeast Asian J Trop Med Public Heal., vol. 23, no. 3, pp. 520–5, 1992.
[8]
OIE, “Manual of Standards for Diagnostic Test and Vaccines. Second Edition.,” 1992.
[9]
G. R. Carter, “Studies on Pasteurella multocida.,” Am. J. Vet. Rec., 1955.
[10]
W. M.. Sarker, A.J.; Amin, M.M. and Hossain, “Testing and quality control of poultry vaccines and its monitoring in the field.,” Bangladesh Agril. Uni. Res. Prog., vol. 6, pp. 249–257, 1992.
[11]
A. R. Chowdhury K. A.; Amin M. M.; Sarker A. J. and Ahamed, “Immunization of chickens against fowl cholera with oil¬adjuvanted broth culture vaccine.,” Bangladesh Vet. J., vol. 21, no. 1–2, pp. 63–73, 1987.
[12]
W. L. Tripathy, D. N.; Hasan, L. E. and Myers, “Detection of fowl pox virus antigen in tissue culture by fluorescent antibody technique.,” Avian Dis., vol. 14, no. 4, pp. 810–812, 1970.
[13]
Derieux, W. T. 1984. Response of Broiler - Type Chickens to Live Pasteurella multocida: Duration of Immunity and Minimum Dose. Avian Diseases, 28: 281–284.
[14]
Wu, Z.J.; Wu, L.Q. and Cai, B. X. 1986. Comparison between primary and secondary immune responses in chickens vaccinated with fowl cholera attenuated vaccine prepared from Pasteurella multocida strain 807. Ani. Husb. and Vet. Med China. 18: 54-56.
[15]
Rahman MA, Islam MB, Samad MA. Efficacy of formalin killed fowl cholera vaccine in experimentally immunized Fayoumi chickens. Bangl. J. Vet. Med. 2004; 2(1): 23–25.
[16]
Avakian AP, Dick JW and Derieux. Fowl Cholera Immunity Induced by Various Vaccines in Broiler Minibreeder Chickens Determined by Enzyme - Linked Immunosorbent Assay. Avian Dis. 1989; 33:97–102.
[17]
Shilpa PC, Verma S. Pathology of Pasteurella multocida infection in chickens. Indian - Journal - of - Animal - Research. 2006; 40(1): 15–19.
[18]
Collins FM. Mechanisms of acquired resistance to Pasteurella multocida infection: a review. Cornell Vet. 1977; 67(1): 103–38.
[19]
Maheswaran SK, Thies ES. Studies on Pasteurella multocida. III. In vitro assay for cell - mediated immunity. Avian Dis. 1976; 20(2): 332–41.
[20]
Mondal SK, Choudhury KA, Amin MM, Rahman MM and Sarker AJ. Immune response of Chicken induced by Alum precipitated fowl cholera vaccine. Bang. Vet. J. 1988; 22(71) 63–69.
[21]
Coates RS, Jensen JM and Brown ED. The response of turkey to varying doses of live oral P. multocida vaccine. Poult. Sci. 1977; 56: 273–276.
[22]
Bhasin JL, Fowl cholera in turkeys - the efficacy of adjuvant bacterins. Avian Dis. 1968; 12: 1.
[23]
Rahman KACMK, Rahman MB, Siddiky MNA, Kafi MA, Islam MA. Efficacy of formalin killed fowl cholera vaccine in experimentally immunized Fayoumi chickens. Bangl.J. Vet. med. 2004; 2(1): 23–25.
