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Coetaneous Serum Supplementation of in vitro Culture Media Improves Morphogenesis and Development Gastrulation Embryos Rabbit
Current Issue
Volume 2, 2015
Issue 5 (September)
Pages: 47-52   |   Vol. 2, No. 5, September 2015   |   Follow on         
Paper in PDF Downloads: 44   Since Sep. 29, 2015 Views: 1648   Since Sep. 29, 2015
Authors
[1]
J. S. Vicente, Institute for Animal Science and Technology, Polytechnic University of Valencia, Valencia, Spain.
[2]
A. Parrilla-Ocon, Institute for Animal Science and Technology, Polytechnic University of Valencia, Valencia, Spain.
[3]
A. M. Saeed, Animal Production Research Institute, Animal Biotechnology Department, Dokki, Giza, Egypt.
[4]
F. Marco-Jimenez, Institute for Animal Science and Technology, Polytechnic University of Valencia, Valencia, Spain.
Abstract
The current study was conducted to investigate the role of blood serum from coetaneous pregnancy rabbit does on in vitro development of rabbit late blastocyst. Embryos at 6 days post-coitum were recovered from nulliparous rabbit does. Normal recovered late blastocysts were cultured in TCM 199 (Control group), TCM199 supplemented with 6 days post-coitum serum blood (10% v/v, Serum group) or TCM199 supplemented with 9 ηg of progesterone water soluble (Steroid group). After 48h culture, a significant differences on the rate of embryos reaching the advanced gastrulation stages 4 to 7 were observed. Medium supplemented with coetaneous serum showed the highest rate with a 73.7% vs media with supplemented with progesterone o without supplementation (47.4% and 48.4%, P<0.05, respectively). Furthermore, blastocysts dimensions were significantly increased after 48h of culture in coetaneous serum or progesterone supplemented medium, and dead rates of blastocyst was higher in no supplemented medium than in supplemented one. In conclusion, coetaneous serum supplementation improves the gastrulation progress of rabbit blastocyst of 6 days old, reaching at 48h of culture, gastrulation states analogous to in vivo. While, progesterone supplementation facilitate the blastocyst growth but not the gastrulation process. Supplementation of medium with coetaneous blood serum may facilitate the study of autonomous part of embryonic differentiation and to analyze by steps the interaction complex mechanism between conceptus and endometrium leading to success or failure of implantation process.
Keywords
Blastocyst, Progesterone, Serum, Conceptus, Implantation
Reference
[1]
Adams CE. Prenatal mortality in the rabbit Oryctolagus cuniculus. J Reprod Fertil 1960; I: 36-44.
[2]
Santacreu MA, Mocé ML, Climent A, Blasco A. Divergent selection for uterine capacity in rabbits. II. Correlated response in litter size and its components estimated with a cryopreserved control population. J. Anim. Sci 2005; 83: 2303-2307.
[3]
Vicente JS, Llobat MD, Viudes-de-Castro MP, Lavara R, Baselga M, Marco-Jiménez F. Gestational losses in a rabbit line selected for growth rate. Theriogenology 2012; 77: 81-88.
[4]
Dorniak P, Spencer TE. Biological roles of progesterone, prostaglandins, and interferon tau in endometrial functionand conceptus elongation in ruminants Anim. Reprod. 2013; 10: 239-251.
[5]
Spencer TE, Johnson GA, Bazer FW, Burghardt RC. Implantation mechanisms: insights from the sheep. Reproduction 2004; 128: 657-668.
[6]
Spencer TE, Johnson GA, Bazer FW, Burghardt RC. Fetal-maternal interactions during the establishment of pregnancy in ruminants. Soc Reprod Fertil Suppl. 2007;64:379-396.
[7]
Bazer FW, Wu G, Spencer TE, Johnson GA, Burghardt RC, Bayless K. Novel pathways for implantation and establishment and maintenance of pregnancy in mammals. Mol Hum Reprod. 2010; 16: 135-152.
[8]
Kane MT, Morgan PM, Coonan C. Peptide growth factors and preimplantation development. Human Reprod Update 1997; 3: 137-157.
[9]
Crosier AE, Farin PW, Alexander JE, Farin CE. Ultrastructural morphometry of bovine compact morula produced in vivo or in vitro. Biol. Reprod. 2000; 62: 1459-1465.
[10]
Safro E, O’Neill C, Saunders DM. Elevated luteal phase estradiol: progesterone ratio in mice causes implantation failure by creating a uterine environment that suppresses embryonic metabolism. Fertility and Sterility 1990; 54: 1150-1153.
[11]
Forde N, Beltman ME, Duffy GB, Duffy P, Mehta JP, O'Gaora P, Roche JF, Lonergan P, Crowe MA. Changes in the endometrial transcriptome during the bovine estrous cycle: effect of low circulating progesterone and consequences for conceptus elongation. Biol Reprod, 2011; 84: 266-278.
[12]
Carter F, Forde N, Duffy P, Wade M, Fair T, Crowe MA, Evans AC, Kenny DA, Roche JF, Lonergan P. Effect of increasing progesterone concentration from day 3 of pregnancy on subsequent embryo survival and development in beef heifers. Reprod Fertil Dev. 2008; 20: 368-375.
[13]
Clemente M, De La Fuente J, Fair T, Al Naib A, Gutierrez-Adan A, Roche JF, Rizos D, Lonergan P. Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium? Reproduction 2009; 138: 507-517.
[14]
Larson JE, Krisher RL, Lamb GC. Effects of supplemental progesterone on the development, metabolism and blastocyst cell number of bovine embryos produced in vitro. Reprod Fertil Dev. 2011; 23: 311-318.
[15]
Satterfield MC, Bazer FW, Spencer TE. Progesterone regulation of preimplantation conceptus growth and galectin 15 (LGALS15) in the ovine uterus. Biol Reprod. 2006; 75: 289-296.
[16]
Lonergan P. Influence of progesterone on oocyte quality and embryo development in cows. Theriogenology 2011; 76: 594-601.
[17]
Viebahn C, Mayer B, Miething A. Morphology of incipient mesoderm formation in the rabbit embryo: a light- and retrospective electron-microscopic study. Acta Anat. 1995; 154: 99-110.
[18]
Viebahn C, Stortz C, Mitchell SM, Blum M. Low proliferative and high migratory activity in the area of Brachyury expressing mesoderm progenitor cells in the gastrulating rabbit embryo. Development 2002; 129: 2355-2365.
[19]
Blum M, Andre P, Muders K, Schweickert A, Fischer A, Bitzer E, Bogusch S, Beyer T, van Straaten HWM, Viebahn C. Ciliation and gene expression distinguish between node and posterior notochord in the mammalian embryo. Differentiation 2007; 75: 133-146.
[20]
Hassoun R, Püschel B, Viebahn C. Sox17 Expression Patterns during Gastrulation and Early Neurulation in the Rabbit Suggest Two Sources of Endoderm Formation, Cells Tissues Organs 2010; 191: 68-83.
[21]
Estany J, Camacho J, Baselga M, Blasco A. Mixed model methodology for the estimation for the genetic response to selection in litter size of rabbits. Livestock Production Sci. 1989; 21: 67-75.
[22]
Martal J, Chene N, Camous S, Huynh L, Lantier F, Hermier P. Recent developments and potentialities for reducing embryo mortality in ruminants: the role of IFN-tau and other cytokines in early pregnancy. Reprod Fertil Dev. 1997; 9: 355–380.
[23]
Daniel JC Jr. Action of progesterone as a cleavage inhibitor of rabbit ova in vitro. J. Reprod. Fert. 1971; 7: 323-329.
[24]
Kirpatrick JF. Differential sensitivity of preimplantation mouse embryos in vitro to oestradiol and progesterone. J. Reprod. Fert. 1971; 27: 283-285.
[25]
Gupta JS, Dey SK, Dickmann Z. Evidence that "embryonic estrogen" is a factor which controls the development of the mouse preimplantation embryo. Steroids 1977; 29: 363-369.
[26]
Roblero L, Izquierdo L. Effect of progesterone on the cleavage rate of mouse embryos in vitro. J. Reprod. Fertil. 1976; 46: 475-476.
[27]
Dickmann Z, Dey KS, Gupta JS. A new concept: control of early pregnancy by steroid hormones originating in the preimplantation embryos. Vitamins and Hormones 1976; 34: 215-243.
[28]
Bochkani R, Kirchner C. Uteroglobin and the accumulation of progesterone in the uterine lumen of the rabbit. Wilheim Roux´s Archives. 1981; 190: 127-131.
[29]
Neimann H, Elsaesser F. Steroid hormones in aerly pig embryo development. In the mammalian preimplantation embryo: regulation of growth and differentiation in vitro. Ed. Bavister B.D. Plenum Press. New York. 1987: 117-130.
[30]
Walker SK, Hartwich KM, Seamark RF. The production of unusually large offspring following embryo manipulation: concepts and challenges. Theriogenology 1996; 45: 111-120.
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