[1]
J. S. Vicente, Institute for Animal Science and Technology, Polytechnic University of Valencia, Valencia, Spain.
[2]
A. Parrilla-Ocon, Institute for Animal Science and Technology, Polytechnic University of Valencia, Valencia, Spain.
[3]
A. M. Saeed, Animal Production Research Institute, Animal Biotechnology Department, Dokki, Giza, Egypt.
[4]
F. Marco-Jimenez, Institute for Animal Science and Technology, Polytechnic University of Valencia, Valencia, Spain.
The current study was conducted to investigate the role of blood serum from coetaneous pregnancy rabbit does on in vitro development of rabbit late blastocyst. Embryos at 6 days post-coitum were recovered from nulliparous rabbit does. Normal recovered late blastocysts were cultured in TCM 199 (Control group), TCM199 supplemented with 6 days post-coitum serum blood (10% v/v, Serum group) or TCM199 supplemented with 9 ηg of progesterone water soluble (Steroid group). After 48h culture, a significant differences on the rate of embryos reaching the advanced gastrulation stages 4 to 7 were observed. Medium supplemented with coetaneous serum showed the highest rate with a 73.7% vs media with supplemented with progesterone o without supplementation (47.4% and 48.4%, P<0.05, respectively). Furthermore, blastocysts dimensions were significantly increased after 48h of culture in coetaneous serum or progesterone supplemented medium, and dead rates of blastocyst was higher in no supplemented medium than in supplemented one. In conclusion, coetaneous serum supplementation improves the gastrulation progress of rabbit blastocyst of 6 days old, reaching at 48h of culture, gastrulation states analogous to in vivo. While, progesterone supplementation facilitate the blastocyst growth but not the gastrulation process. Supplementation of medium with coetaneous blood serum may facilitate the study of autonomous part of embryonic differentiation and to analyze by steps the interaction complex mechanism between conceptus and endometrium leading to success or failure of implantation process.
Blastocyst, Progesterone, Serum, Conceptus, Implantation
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