Cloning and Expression Profile of PID1 in Yak
[1]
Lin Ya-qiu, College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China.
[2]
Zhao Yan-ying, College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China.
[3]
Li Rui-wen, Reproductive and Endocrine Laboratory, Chengdu Woman-Child Central Hospital, Chengdu, China.
[4]
Zheng Yu-cai, College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China.
[5]
Wang Yong, College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China.
[6]
Zhang Ming, College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China.
To elucidate the role of phosphotyrosine interaction domain containing l (PID1) gene in yak (Bos grunniens), the PID1 gene of Jiulong yak was cloned by RT-PCR and analyzed by bioinformatics method. The tissue and temporal expression profile of PID1 gene of Jiulong yak were detected using semi-quantitative RT-PCR and fluorescence quantitative PCR, respectively. The results showed that the length of Jiulong yak PID1 gene cDNA was 776 bp (GenBank accession number: KC184121) with an ORF of 612 bp which encodes 203 amino acids. The deduced PID1amino acid sequence of Jiulong yak shares a high homology with that of cattle, goat, pig, human, pygmy chimpanzee, mouse, rat, chicken and african clawed frog (89%-100%), while a lower homology with that of zebrafish (71%). Semi-quantitative RT-PCR analysis showed that the PID1 gene expression was observed in heart, liver, spleen, kidney, longissimus muscle and fat of Jiulong yaks. The mRNA level of PID1 in longissimus muscle, heart and liver was significantly higher than that in other studied tissues (P<0.05). The expression levels of PID1 gene in longissium muscle of 0.5 and 9-year Jiulong yaks were significantly higher than that in those yaks at the age of 3.5 - 5.5 years old (P<0.05). These results suggest that PID1 may play an important role in the regulation of meat quality of yak.
PID1, Jiulong Yak, Gene Clone, Tissue Distribution, Temporal Expression
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