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Prevalence, Pathogenic Markers and Antibiotic Susceptibility of Vibrio cholerae in Sardines, Water and Phytoplankton in Lake Tanganyika, Tanzania
Current Issue
Volume 6, 2018
Issue 2 (April)
Pages: 29-34   |   Vol. 6, No. 2, April 2018   |   Follow on         
Paper in PDF Downloads: 60   Since Mar. 13, 2018 Views: 690   Since Mar. 13, 2018
Authors
[1]
Nyambuli Sosthenes, Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Morogoro, Tanzania; National Fish Quality Control Laboratory, Ministry of Livestock and Fisheries, Mwanza, Tanzania.
[2]
Ofred Jonas Mhongole, National Fish Quality Control Laboratory, Ministry of Livestock and Fisheries, Mwanza, Tanzania.
[3]
Abdul AhamedSelemani Katakweba, Pest Management Centre, Sokoine University of Agriculture, Morogoro, Tanzania.
[4]
Anders Dalsgaard, Department of Veterinary Disease Biology, Copenhagen University, Copenhagen, Denmark.
[5]
Robinson Hammerthon Mdegela, Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Morogoro, Tanzania.
Abstract
The aim of this study was to evaluate the extent of Vibrio cholerae contamination and their antibiotic resistance patterns in sardines, water and phytoplankton in Lake Tanganyika, Tanzania. A total of 120 samples from sardines, water and phytoplankton were collected and analysed for V. cholerae. All isolates confirmed as V. cholerae using Polymerase Chain Reaction were also determined for virulence genes and antibiotic susceptibility. Sardine samples (9%) harboured V. cholerae (non-O1) and water samples (3%) harboured V. cholerae O1. Vibrio cholerae was not detected and isolated in phytoplankton samples. One isolate from water samples harboured both toxin regulatory protein (toxR) and haemolysin gene (hlyA), but not cholera enterotoxin gene (ctx) and toxin co-regulated pilus gene (tcpA). Vibrio cholerae isolates were resistant to Ampicillin (83.33%), Amoxicillin (100%), Chloramphenicol (50%) and Tetracycline (100%). All of the isolates were susceptible to Gentamicin and Ciprofloxacin. The study demonstrated that, fish and water are important reservoirs of V. cholerae. Regardless the absence of ctx and tcpA, constant monitoring for V. cholerae should be done as they pose threat to human health.
Keywords
Cholera, Fish, Virulence Genes, Sardines
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