Antifungal Screening of Mimosa Pudica Plant Extracts against Phytopathogenic Fungi
[1]
I. Ibrahim D., Department of Biology, Zamfara State College of Education Maru, PMB 1002, Maru, Zamfara State, Nigeria.
[2]
I. Muhammad, Department of Chemistry, Zamfara State College of Education Maru, PMB 1002, Maru, Zamfara State, Nigeria.
[3]
A. I. Kanoma, Department of Biology, Zamfara State College of Education Maru, PMB 1002, Maru, Zamfara State, Nigeria.
[4]
K. Shehu, Department of Biological Science, Usmanu Dan Fodiyo University Sokoto PMB 2346, Sokoto, Nigeria.
[5]
A. A. Aliero, Department of Biological Science, Usmanu Dan Fodiyo University Sokoto PMB 2346, Sokoto, Nigeria.
[6]
R. U. Aliyu, Department of Biochemistry, Usmanu Dan Fodiyo University Sokoto PMB 2346, Sokoto, Nigeria.
The antifungal properties of the leaf and stem of Mimosa pudica plant (Touch me not) was investigated using agar incorporation method. The activity was tested against Trichophyton verrocusum, T. mentagrophytes, Microsporum nanum, Aspergillus niger and A. flavus at 10, 20 and 40 mg/ml. The extracts inhibited the growth of the above mentioned fungi at 10, 20 and 40 mg/ml except A. niger. The highest growth inhibition (90%) was observed at 40 mg/ml of leaf methanolic extract against T. verrocusum. Generally, the results showed significant difference (P<0.05) in the percentage growth inhibition of leaf, stem and mixture of M. pudica on all the fungal isolates at the different concentrations of the extracts. Antifungal activity against the isolates were observed to increase significantly (P<0.05) with increasing concentrations of plant extracts. The highest antifungal activity of the extracts was recorded at 40mg/ml concentration, which was significantly higher than that of the positive control (Greseofulvin, 10mg/ml), but lower than Greseofulvin at 40 mg/ml concentration. The plant extracts of the leaves, stem and mixture did not show any antifungal activity against A. niger at the various concentrations used in this study. It is therefore suggested that the local ethnomedical preparation and prescription of the plant sources should be scientifically evaluated and then disseminated properly and the knowledge about botanical preparation of traditional sources of medicinal plants should be extended for future investigation for drugs discovery.
Antifugal Activity, Mimosa Pudica Plant, Aqueous Extract, Methanol Extract, Ethanol Extract
[1]
Ahmad, I, and Beg, A. Z. (2001).Antimicrobial and phytochemical Studies on 45 Indian Medicinal plants Against Multi drug resistant Human Pathogens.J Ethanopharmacol 74:113-23.
[2]
Bailey, R. A. (2008). Design of Comparative Experiment. Cambridge University Press. ISBN 978-0-521-68357-9.
[3]
Banso A. and Mann A. (2008). Evaluation of Antibacterial Properties of Flavonoid Fraction from Antiaris africana (Engl). Journal of Applied Biosciences, 12: 665 - 670.
[4]
Bigal, H.G. (2010). The Sensitive Plant Mimosa PudicaFile:IIC:/Documents and Settings/User/My Documents/BBC/-h2g2 the Sensitive Retrieved on 5/13/2010. Pp 1-4.
[5]
Cartney,Y.and Markie, MC. (1999). Practical Microbiology. Edn 14 Harcourt Brace Company Limited (PP 695 – 696).
[6]
Dale, M. M. and Mandelstam, J. (2009) Antibiotics. Mi-crosoft ® Encarta ® (2009) (DVD) Redmond, W.A Microsoft Corporation 2008. Pp 2-4.
[7]
Das, S Pal, S., Mujib, A. and Dey, S. (1999) Biotechnology of medicinal plants- Recent Advanced and Potential. 1st Edition, Vol II (UK992 Publications Hyderabad), 126-139.
[8]
Deininger, R, (1984), Lectures of the Medical Congress Berlin: Firma Klosterfrau, Koln.24-31.German Resources Information Network (2008).”Mimosa pudica L.”. GRN, United States Department of Agriculture, Agricultural Resoure service, Beltsville Area. Pp 8-10.
[9]
Doss, A., Vijayasanthi, M., Parivu-guna, V. and Anand, S.P. (2011). Evaluation of antibacterial properties of ethanol and flavonoids from Mimosa pudica Linn. and Panicum maximum Jacq. Plant Sciences Feed. 1(2): 39- 44.
[10]
Dwivedy, S.K. and Dubey, N.K. (1983). Potential use of the essential oil Tachysperman ammi against seed borne fungi of Gvar (Cyamopsii tetragonoloba, L. Talib). Mycopathology, 121: 101-104.
[11]
Hartman, G. R. and Rohde (1980). Introducing Mycology – Dermatophytes. PP 8 – 14.
[12]
Khan, S., Khan, G. M., Mehsud, S., Rahman, A. and Khan, F. (2004). Antifungal Activ-ity of Tamarix dioica – an in vitro study. Gomal Journal of Medical Sciences. 2 (2):40-42.
[13]
Leven, M., Venden – Berghe, D .A., Morten, I., Vilientrick and A. Lomweas, E.C (1979). Screening of higher plants for biological activity. Planta Medica, 36: 311-312.
[14]
Montgometry Douglas, C. (2001). Design and Analysis of Experiments, 5thed. New York Wiley. ISBN 9780471316497.
[15]
Ofodile, L. N., Kanife, U. C. and Arojoye, B.J. (2010). Antifungal activity of a Nigeria herbal plant Chrysanthellum americanum. Journal of Life and Physical Sciences acta SATECH 3 (2):60-63.
[16]
Ojalla, T., Remes, S, and Hans, P. (1999). Antimicrobial activity of some coumarin containing herbal plants growing in Finland. J thnophar-macology, 68(3):267-274.
[17]
Palacious, C. and Reyes, R. E. ( 1991). Antibacterial and Anti-mycotic of Mimosa pudica in Experimental Animals, Arch Invest Med.(Mex) 22(2) : 163-169.
[18]
Shale, T.L. Stick, W.A. and Van stan, (1999).Screening of Medicinal plant used in Lesotho for antibacterial and anti inflammatory activity. J. Ethanopharmacol 67: 847 – 354.
[19]
Shelef, L. A. (1983). Antimicrobial effects of spices. J. Food Safety 6: 29-44.
[20]
Stevia and Rebs, A. G. (2008). Mimosa pudica (Makahiya) http://findmeacure 2008/11/17/ mimosa-pudica. Retrieved on 4/29/2010.
[21]
Suresh, B., Kalyamaraman, V. R. and Dhahasekaran, S. (1994). Evaluation of Certain Siddha Drugs in the Treatment of Candidiasis Acient Science of Lif.
[22]
Tsuchiya,H.,Sato,M.,Mayazaki,T.,Fujiwara,S.,Tanigaki, S.,Ohyama,M., Tanaka,T., and Linuma, M., (1996). Comparative Study on the Antibacterial Activity of Phyto-chemical Flavanones against Methicillin-Resistant Staphylococcus aureus. Journal of Ethnophar-macology, 50:27–34.
[23]
Vogel H. G. (1991). Similarities between Various systems of Tradi-tional Medicine. Considerations for the future of ethnopharmacology. J Ethnopharmacol. 35: 179–90.
[24]
Zaika, L. L. (1988). Spices and Herbs: Their Antimicrobial Activity and Its Deter-mination. J. Food Safety. 9:97-118.