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FTIR and GC-MS Analysis of the Aqueous and Ethanolic Extracts of Jatropha tanjorensis Leaves
Current Issue
Volume 8, 2021
Issue 1 (March)
Pages: 1-11   |   Vol. 8, No. 1, March 2021   |   Follow on         
Paper in PDF Downloads: 39   Since Jan. 11, 2021 Views: 706   Since Jan. 11, 2021
Authors
[1]
Mayel Mida Habila, Deparment of Biochemistry, Federal University Wukari, Taraba, Nigeria.
[2]
Ezeadina Arinze Festus, Deparment of Biochemistry, Federal University Wukari, Taraba, Nigeria.
[3]
Daji Morumda, Department of Microbiology, Federal University Wukari, Taraba, Nigeria.
[4]
Ikwebe Joseph, Deparment of Biochemistry, Federal University Wukari, Taraba, Nigeria.
[5]
Anih David Chinonso, Deparment of Biochemistry, Federal University Wukari, Taraba, Nigeria.
[6]
Abu Michael Sunday, Deparment of Biochemistry, Federal University Wukari, Taraba, Nigeria.
Abstract
Background and Objective: The exploitation of plants for food and as biopharmaceuticals to remedy diseases in traditional and herbal medicine has, over the years, raised concerns as they, at times, have negative side effects. This calls for proper phytoanalysis and screening of such plants, one of which is Jatrophha tanjorensis (J. tanjorensis), to ascertain their informed use. This research aimed at investigating the phytocomponents of Jatropha tanjoresis leaves. Materials and Methods: Fresh J. tanjorensis leaves were collected from around New Market, Wukari, Taraba State, Nigeria. Aqueous and ethanolic (70% and 95%) extracts of J. tanjorensis leaves were analyzed using GC-MS Clarus 500 Perkin Elmer system. Identification of functional groups in J. tanjorensis leaves (dried, powdered) was done using FT-IR spectroscope (Shimadzu, IR Affinity 1, Japan). Proximate analysis of the leaves was also by AOAC methods. Results: FT-IR analysis of J. tanjorensis leaves revealed the presence of phytochemicals like alkanes, alkenes, alkyls, alkyl halides, alkynes, saturated aliphatic esters, primary amines, aromatics, nitro compounds, aromatic amines, aliphatic amines. From the GC-MS analyses, the aqueous extract had the highest number (51 phytochemicals) of phytochemicals, followed by 95% ethanolic exract (up to 31 phytochemicals), and lastly 95% ethanolic extact (up to 26 phytochemicals). However, the phytochemicals varied considerably from extract to extract both in nature/type and in abundance. Proximate results obtained are moisture content (0.65%), ash (9.8%), crude protein (38.56%), fat (19.60%), crude fibre (18.71%) and carbohydrate (12.68%). Conclusion: J. tanjorensis leaves are a recommendable source of useful bioactive components and other phytochemicals that can be exploited, probably by chemically modifications, as pharmaceuticals/drugs. We recommend, therefore, the use of J. tanjorensis leaves as part of diet.
Keywords
Phytocomponents, Proximate, Bioactive, Spectroscopy, Functional Groups
Reference
[1]
Chigozie OO, Uzoma NO, Ikechukwu UR, Ikechukwu ES. Nutritional composition of jatropha tanjorensis leaves and effects of its aqueous extract on carbon tetrachloride induced oxidative stress in male Wistar albino rats. Biomed Res. 2018; 29 (19): 3569–76.
[2]
Souza ENF, Williamson EM, Hawkins JA. Which plants used in ethnomedicine are characterized? Phylogenetic patterns in traditional use related to research effort. Front Plant Sci. 2018; 9 (June): 1–12.
[3]
Aziz MA, Adnan M, Khan AH, Shahat AA, Al-Said MS, Ullah R. Traditional uses of medicinal plants practiced by the indigenous communities at Mohmand Agency, FATA, Pakistan. J Ethnobiol Ethnomed. 2018; 14 (1): 1–16.
[4]
Kigen G, Kamuren Z, Njiru E, Wanjohi B, Kipkore W. Ethnomedical Survey of the Plants Used by Traditional Healers in Narok County, Kenya. Evidence-based Complement Altern Med. 2019.
[5]
Falodun A, Udu-Cosi AA, Erharuyi O, Imieje V, Falodun JE, Agbonlahor O, et al. Jatropha Tanjorensis - Review of Phytochemistry, Pharmacology, and Pharmacotherapy. J Pharm Allied Sci. 2013; 10 (3): 1955–64.
[6]
Prabakaran AJ, Sujatha M. Jatropha tanjorensis Ellis & Saroja, a natural interspecific hybrid occurring in Tamil Nadu, India. Genet Resour Crop Evol. 1999; 46: 213–8.
[7]
Oboh FOJ, Masodje HI. Nutritional and Antimicrobial properties of Jatropha tanjorensis leaves. Am J Sci Res. 2009; 4 (1): 7–10.
[8]
Iwalewa EO, Adewumi CO, Omisore NO, Adebanji OA, Azike CK. Proantioxidant effects and cytoprotective potentials of nine edible vegetables in Southwest. Niger J Med Food. 2005; 8: 539–44.
[9]
Omoregie ES, Osagie AU. Antioxidant Properties of Methanolic Extracts of some Nigerian Plants on Nutritionally-Stressed Rats. Niger J Basic Appl Sci. 2012; 20 (1): 7–20.
[10]
Olayiwola GO, Iwalewa EO, Omobuwajo OR, Adebajo CO, Adeniyi AA, Verspohl EJ. Antidiabetic potential of Jatropha tanjorensis leaves. Nigerian Journal of Natural Products and Medicine. 2004; 8: 37–401.
[11]
Omoregie ES, Osagie AU. Phytochemical screening and antianaemic effect of Jatropha tanjorensis leaf in protein malnourished rats. Plants Archeol. 2007; 7: 509–16.
[12]
Omobuwajo O, Alade G, Akanmu M, Obuotor EM, Osasan S. Microscopic and toxicity studies on the leaves of Jatropha tanjorensis. African J Pharm Pharmacol. 2011; 5: 12–7.
[13]
Fraekel GS. “The raison d’Etre of secondary plant substances.” Science (80-). 1959; 129 (3361): 1466–70.
[14]
Balunas MJ, Kinghorn AD. Drug Discovery from medicinal Plants. Life Sci. 2005; 789 (5): 431–41.
[15]
Omoregie ES, Sisodia BS. In vitro antiplasmodial activity and cytotoxicity of leaf extracts from Jatropha tanjorensis. Pharmacol Online. 2012; 2: 656–73.
[16]
Sule WF, Okonko IO, Omo-Ogun S, Nwanze J, Ojezele MO, Ojezele OJ, et al. Phytochemical properties and in-vitro antifungal activity of Senna alata Linn. crude stem bark extract. J Med Plants Res. 2011; 5 (2): 176–83.
[17]
Hema R, Kumaravel S, Gomathi S, Sivasubramaniam C. Gas Chromatography-Mass Spectroscopic analysis of Lawsonia inermis leaves. New York Sci J. 2010; 3: 141–3.
[18]
Mathivanan D, Gandhi PR, Mary RR, Suseem SR. Larvicidal and acaricidal efficacy of different solvent extracts of Andrographis echioides against blood-sucking parasites. Physiol Mol Plant Pathol [Internet]. 2017; 101: 187–96. Available from: http://dx.doi.org/10.1016/j.pmpp.2017.03.008
[19]
Anayo Jose U, Oluchi Hel U, Friday Nwa N, Obasi Uche O, Ikechukwu I, Nzubechukw E, et al. Phytochemical and GC-MS Evaluation of Bioactive Principle of Vitis vinifera Peels. Asian J Appl Sci. 2018; 11 (4): 192–8.
[20]
Sabzar AD, Farooq AG, Abdul RY, Masood ul Hassan B, Towseef MB, Farooz AB. Bioactive potential of leaf extracts from Urtica dioica L. against fish and human pathogenic bacteria. African J Microbiol Res. 2012; 6 (41): 6893–9.
[21]
Lubna A, Ali RA, Siddiqui F, Fazil P, Farooq AD, Siddiqui AJ. Cytotoxic activity of extracts of Ixora species and their GC-MS analysis. J Chem Soc Pakistan. 2018; 40 (5): 3–5.
[22]
Gresta F, Cristaudo A, Spampinato G, Catara S, Galesi R, Napoli E, et al. Morphological traits and aromatic profile of Crocus biflorus Mill. Acta Hortic. 2017; 1184: 211–8.
[23]
El-azim MHMA, Yassin FA, Khalil SA, El-mesalamy AMD. Hydrocarbons, fatty acids and biological activity of date palm pollen (Phoenix dactylifera L.) growing in Egypt. IOSR J Pharm Biol Sci. 2015; 10 (3): 46–51.
[24]
Guedes AP, Franklin G. Hypericum sp.: essential oil composition and biological activities. Phytochem Rev. 2012; 11 (2012): 127–52.
[25]
Chauhan RS, Vashistha RK, Nautiyal MC, Tava A, Cecotti R. Essential Oil Composition of Hypericum perforatum L. from Cultivated Source Essential Oil Composition of Hypericum perforatum L. from Cultivated Source. J Essent Oil Res. 2011; 23 (3): 20–5.
[26]
Bharathy V, Uthayakumari F. Bioactive Components in leaves of Jatropha tanjorensis J. L. Ellis & Saroja by GC-MS Analysis. Int J PharmTech Res. 2013; 5 (4): 1839–43.
[27]
Obici S, Feng Z, Morgan K, Stein D, Karkanias G, Rossetti L. Central Administration of Oleic Acid Inhibits Glucose Production and Food Intake. Diabetes. 2002; 51: 271–5.
[28]
Zhang J, Villacorta L, Chang L, Fan Z, Hamblin M, Zhu T, et al. Nitro-Oleic Acid Inhibits Angiotensin II – Induced Hypertension. Circ Res. 2010; 107 (4): 540–8.
[29]
Preethi P, Soorianathasundaram K, Subramanian KS. AROMA VOLATILE COMPOUNDS OF MANGO CULTIVARS NEELUM AND BANGANAPALLI. Biochem Cell Arch. 2014; 14 (2).
[30]
Garaniya N, Bapodra A. E thno botanical A review and P hytophrmacological potential of A brus precatorius L.: Asian Pac J Trop Biomed. 2014; 4 (Suppl 1): S27–34.
[31]
Hassan WHB, El-Gamal AA, El-sheddy E, Al-oquil M, Farshori NN. The chemical composition and antimicrobial activity of the essential oil of Lavandula coronopifolia growing in Saudi Arabia. J Chem Pharm. 2014; 6 (2): 604–15.
[32]
Ramdani M, Lograda T, Chalard P, Figueredo G, Laidoudi H. Chemical composition, Antimicrobial activity and chromosome number of Urospermum dalechampii from Algeria. Sch Acad J Pharm. 2014; 3 (April): 477–82.
[33]
Arora S, Kumar G. Phytochemical screening of root, stem and leaves of Cenchrus biflorus Roxb. 2018; 7 (1): 1445–50.
[34]
Kumar MH, Prabhu K, Ram M, Rao K, Shanthi B, Kavimani M. Gas chromatography/mass spectrometry analysis of one Ayurvedic skin oil, Eladi Kera Thailam. Trug Invent Today. 2019; 11 (10): 2657–60.
[35]
Velmurugan G, Anand SP. GC-MS Analysis of Bioactive Compounds on Ethanolic Leaf Extract of Phyllodium pulchellum L. Desv. Int J Pharmacogn Phytochem Res. 2017; 9 (1): 114–8.
[36]
Adeyemi MA, Ekunseitan DA, Abiola SS, Dipeolu MA, Egbeyale LT, Sogunle OM. Phytochemical Analysis and GC-MS Determination of Lagenaria. Int J Pharmacogn Phytochem Res 2017; 2017; 9 (7): 1045–50.
[37]
Iyer S, Sam FS, Diprimio N, Preston G, Verheijen J, Murthy K, et al. Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG. Dis Model Mech. 2019; 12: 1–12.
[38]
Ayogu RNB, Nnam NM, Mbah M. Evaluation of two local cowpea species for nutrient, antinutrient, and phytochemical compositions and organoleptic attributes of their wheat-based cookies. Food Nutr Res. 2016; 60: 1–8.
[39]
Ordinioha B, Brisibe S. The human health implications of crude oil spills in the Niger delta, Nigeria: An interpretation of published studies. Niger Med J. 2013; 54 (1): 10.
[40]
Chikwendu JN, Igbatim AC, Obizoba IC. Chemical Composition of Processed Cowpea Tender Leaves and Husks. Int J Sci Res Publ. 2014; 4 (5): 1–5.
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