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Biochemical and Histopathological Assessment of Organs of the African Catfish (Clarias gariepinus Burchell 1822) Juveniles Exposed to Municipal Sewage Effluent
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Volume 8, 2021
Issue 2 (June)
Pages: 19-29   |   Vol. 8, No. 2, June 2021   |   Follow on         
Paper in PDF Downloads: 61   Since Jun. 30, 2021 Views: 815   Since Jun. 30, 2021
Samuel Olusayo Akinkuolie, Institute of Ecology and Environmental Studies, Obafemi Awolowo University, Ile-Ife, Nigeria.
Henry Adefisayo Adewole, Department of Zoology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
Promise Olabode Ololade, Department of Zoology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
Bola Morufat Lawal, Nigeria Institute of Oceanography and Marine Research, Victoria Island, Lagos, Nigeria.
Victor Folorunso Olaleye, Department of Zoology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
The study evaluates the biochemical and histological changes that occurred in some organs of African catfish (C. gariepinus) exposed to varying concentration of municipal sewage. Two hundred and twenty (220) fingerlings of C. gariepinus (7.65 ± 0.02 g) were randomly stocked in five glass tank in duplicate at 10 fish per tank with varying sewage concentration of 8.24%, 4.12%, 2.06%, 1.03% and 0% for 90 days. The raw sewage, gills and muscles of the exposed fish were analysed for heavy metal content. Also, the muscle, liver and gill of the exposed fish were assayed for Total Protein (Tp), Aspartate transaminase (AST), Alanine transaminase (ALT), Glutathione Peroxidase (GPx), Glutathione Reductase (GR), Glutathione-s-transferase (GST) using standard methods. The order of heavy metals analysed in the sewage were Fe > Cu > Zn > Pb > Cr > Cd. However, heavy metal accumulation in the muscle was found to be in the order Cu > Fe > Cd > Cr > Zn > Pb while that of gills followed the order Fe > Cu > Zn > Cd > Cr > Pb. The activities of the assayed enzymes showed a general increase in hepatic enzymes ALT and AST in the organs of the fish juvenile which was significant (p<0.05) mostly at the higher concentrations (4.12% and 8.24%) while the antioxidant enzymes showed a generally depressed activity with increase in sewage concentrations. At the end of the culture period, mortality was highest in the treatment with 8.24% concentration. Liver histopathology revealed anomalies and alterations within the architecture of test fish liver which was consistent upon increased concentration of sewage. The study concluded that municipal sewage concentrations had adverse effect on fish eco-physiology.
Heavy Metal, Bioaccumulation, Municipal Effluent, Enzymes, Histological Alteration, Eco-physiology
Taiwo, A. M. (2011). Composting as a sustainable waste management technique in developing countries. Journal of Environmental Science and Technology. 4 (2): 93-102.
Babatunde B. B., Vincent-Akpu I. F., Woke G. N., Atarhinyo E., Aharanwa U. C., Green, A. F., and Isaac-Joe O. (2013). Comparative analysis of municipal solid waste (MSW) composition in three local government areas in Rivers State, Nigeria. African Journal of Environmental Science and Technology. 7: 874-881.
Martin, J. C., Hoggart, C. and Matissa, A. (1998). Improvement priorities for sewage treatment in Latvian small and medium sized towns. Water Science Technology, 37 (8): 137-144.
Izonfuo, L. W. A. and Bariweni, A. P. (2001). The effect of urban runoff water and human activities on some physico- chemical parameters of the Epie Creek in the Niger Delta. Journal of Applied Science and Environmental Management 5 (1): 47-55.
Langston, W. J., Burt, G. R. and Pope, N. D. (1999). Bioavailability of metals in sediments of the dogger bank (Central North Sea): A mesocosm study. Estuarine, Coastal and Shelf Science 48: 519-540.
Steven, M. A, Robert S. and Rogers, C. (2003). Assessing the Impact of Environmental Management Systems on Corporate and Environmental Performance. Journal of Operations Management. 21: 329-351.
Alam, Z., Muyibi, S. A. and Toramae, J. (2006). Statistical optimization of adsorption processes for removal of 2,4-dichlorophenol by activated carbon derived from oil palm empty fruit bunches. Journal of Environmental Sciences. 19 (6): 674-677
Mvungi, A., Hranova, R. K., Love, D., 2003. “Impact of home industries on water quality in a tributary of the Marimba River, Harare: implications for urban water management,” Physics and Chemistry of the Earth, 28 (20-27): 1131–1137.
Tam, N. F. Y. and Wong, Y. S. 2000. Spatial variation of heavy metals in surface sediments of Hong Kong mangrove swamps. Environmental Pollution 110: 612-622.
Ekpo, K. E., Asia, I. O., Amayo, K. O., and Jegede, D. A. (2008). Determination of lead, cadmium and mercury in surrounding water and organs of some species of fish from Ikpoba River, Benin City, Nigeria. International Journal of Physical Science. 3: 289-292.
Olaifa, F. E., Olaifa, A. K., Adelaja, A. A., and Owolabi, A. G. 2004. Heavy Metal contamination of Clarias gariepinus from a lake and fish farm in Ibadan, Nigeria. African Journal of Biomedical Research 7: 145-148.
Siroka, Z. and Drastichova, J., 2004. Biochemical Markers of Aquatic Environment Contamination- Cytochrome P450 in fish. A Review. Acta Veteriniria Brno 73: 123-132.
El-Sayed, A. E., Mohamed, S. E., El-Sayed, N., and Zeinab, Z. K. K. 2011. Assessment of heavy metals in water, sediment and fish tissues, from Sharkia province, Egypt. Journal of Aquatic Biology and Fish 15 (2): 125- 144.
Mondon, J. A., Duda, S., Nowak, B. F. 2001: Histological, growth and 7-ethoxyresorufin O-deethylase (EROD) activity responses of greenback flounder Rhombosolea tapirina to contaminated marine sediment and diet. Aquatic Toxicology 54: 231-247.
Faverney, R., Devaux, A., Lafaurie, M., Girard, J. P., Rahmani, R., 2001: Toxic effects of wastewater collected at upstream and downstream sites of a purification station in cultures of rainbow trout hepatocytes. Archives of Environmental Contamination and Toxicology 41. 129-141.
Slatinska, I., Smutna, M., Havelkova, M., Svobodova, Z. 2008. Review article: Biochemical markers of aquatic pollution in fish - Glutathione S-Transferase. University of Veterinary and Pharmaceutical Sciences Brno, Palackeho l-3, 612 42 Brno The Czech Republic. folia veterinaria, 52 (3-4): 129-134.
Van der Oost, R.; Beyer, J. and Vermeulen, N. P. E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environmental Toxicology and Pharmacology. 13: 57-149.
Reddy, P. B., and Waskale, K. 2013: Using histopathology of fish as a protocol in the assessment of aquatic pollution. Journal of Environmental Research and Development 8 (2): 371-375.
Velkova, J. L., and Kostoski, G. (2005). Histopathological analysis of liver in fish (Barbus meridionalis petenyi Heckel) in reservoir Trebenista. Nat. Croat., 14 (2): 147–153.
Rand, G. M. 2008. Fish toxicity studies. In: Di Giulio, R. T., Hinton, D. E. (Eds.), The Toxicology of Fishes. CRC Press, New York, USA, pp. 659–682.
Ademoroti, C. M. A. (1996b). Standard Methods for Water and Effluents Analysis, Foludex Press Ltd., Ibadan.
APHA (American Public Health Association), AWWA (American Water Works Association), WPCF (Water Pollution Control Facilities) (1992). Standard Methods for the Examination of Water and Wastewater, 18th Ed. American Public Health Association Washington, D. C.: 1289.
Habig, W. H., Pabst, M. J. and Jakoby, W. B (1974). Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation Journal of Biological Chemistry 249: 7130-7139.
Glatzle, D, Korner W. F., Christeller, S., and Wiss, O., (1970) Method for the detection of a biochemical riboflavin deficiency. Stimulation of NADPH2-dependent glutathione reductase from human erythro-cytes by FAD in vitro. Investigations on the vitamin B2 status in healthy people and geriatric patients. International Journal of Vitamin Research 40: 166–183.
Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G. and Hoekstra, W. G. (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science 179: 588–590.
Reitman, S. and Frankel, S. (1957). A colorimetric method for the determination of serum oxaloacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology 28: 56-63.
Schmidt, E. and Schmidt, F. W. (1963). Determination of serum GOT and GPT. Enzyme. Biol. Clin. 3: 1.
Tietz. N. W., Grant, G. H. (1995). Amino acid and protein, fundamentals of clinical chemistry, Tietz. N. W. Editor, Third Edition, W. B. Saunder company Philadelphia USA328-1987.
Avwioro, G (2011). Histochemical uses of haematoxylin – A review. Journal of Chemical and Pharamceutical Sciences 1: 24-34.
Kelly W. R. (1979): Veterinary Clinical Diagnosis 2nd ed. Balliere Tindall, London pp. 266-279.
Zar, J. H. (2001). Biostastistical Analysis Prentice – Hall Englewood Cliffs, New Jersey, USA p. 300.
FEPA (1991) Guidelines and standards for Environmental Pollution control in Nigeria. Federal Environmental Pollution Agency (FEPA), Nigeria 550 pp.
National Environmental Standards and Regulation Enforcement Agency, NESREA (2009). Federal Republic of Nigeria Official Gazette, National Environmental (Sanitation and Waste Control). Federal Government of Nigeria Printer, Abuja, Nigeria, FGP 112/102009/L000 (OL54). No. 60 (96): 1057 – 1102.
USEPA, (2009). Drinking Water Contaminants. Washington, DC. (Available online: http://water.epa.gov/drink/contaminants/index.cfm#Lis.)
WHO (1996). Guidelines for drinking water quality (2nd ed.): Health Criteria and supporting information WHO, Geneva, Switzerland.
Inyang, I. R., 2008. Hematological and biological responses of Clarias gariepinus exposed to diazinon. Ph.D. Thesis, Rivers State University of Science and Technology, Port Harcourt, Nigeria, pp: 70-78.
Sastry, K. V. and Agarwal, M. K. 1979. Effect of lead nitrate on the activities of a few enzymes in the kidney and ovary of Heteropneusteus fossilis. Bulletin of Environmental Contamination and Toxicology 22: 055 – 059.
Das, B. K., and Mukherjee, S. C. 2000. Sub-lethal effects of Quinalphos on selected blood parameters of Labeo rohita (Ham) fingerlings. Asian Fisheries Science. 13: 225-233.
Jee, L. H., Masroor, F and Kang, J. 2005. Responses of Cypermethrin induced stress in haematological parameters of Korean rockfish, Sebastes schegeli. Aquactic Research., 36: 898-905.
Ayalogu, O. E., Igbon, N. M., and Dede, E. B. (2001). Biochemical changes in the serum and liver of albino rats exposed to petroleum samples (gasoline, kerosene and crude petroleum). Journal of Applied Science and Environmental Management. 5 (1): 97-100.
Svoboda M, Luskova V, Drastichova J, and Llabok V (2001). The effect of diazinon on hematological indices of common carp (Cyprinus carpio). Acta Veteriniria (Brno) 70: 457-465.
Tiwari S, Singh A (2004): Piscicidal activity of alcoholic extract of Nerium indicum leaf and their biochemical stress response on fish metabolism. African Journal of Traditional Complementary and Alternative Medicines. 1: 15-29.
Shoemaker, C. A., Klesius, P. H., Lim, C., and Yildirim, M. (2003): Feed deprivation of channel catfish, Ictalurus punctatus (Rafi-nesque), influences organosomatic indices, chemical composition and susceptibility to Flavobacterium columnare. Journal of Fish Diseases 26 (9): 553-61.
Sayeed, I., Parveez, S., Bin-Hafeez, B., Haque, R., and Raisuddin, S. 2003. Oxidative stress biomarkers of exposure to delta methrin in freshwater fish, Chenna punctatus Bloch. Ecotoxicology and Environmental Safety 56: 295-301.
Sreejai, R and Jaya, D. S. 2010. Studies on the changes in peroxidation and antioxidants in fishes exposed to Hydrogen Sulphide. Toxicology International 17 (2): 71-77.
Abdel-Moneim, A, M., Abou Shabana, N. M., Khadre, S. E. M. and Abdel-Kader, H. H (2008). Physiological and histopathological effects in catfish Clarias lazeraiI exposed to dyestuff and chemical wastewater. International Journal of Zoological Research 4: 189-202.
Triebskorn, R., Kohler, H. R., Honnen, W., Schramm, M, Adams, S. M. and Muller, E. F. (1997). Induction of heat shock proteins, changes in liver ultrastructure and alterations of fish behavior: are these biomarkers related and are they useful to reflect the state of pollution in the field? Journal of Aquatic Ecosystem Health, 6: 57-73.
Meyers, M., Johnson, L., Hom, T., Collien, T., Stein, J., Varansi, U and Washington, U (1998). Toxipathic hepatic lesion in subadult English sole (Pleurinectes vetulus) from Puget sound: relationship with other biomarkers of contaminant exposure. Marine environmental Research 45: 47-67.
Carolina, C. S., Tatiane, O. B., Sydnei, M. S., Adlair, W. J., Maria, M. F., Olguita, G. F. R., Silvia, D. C and Wagner, L. T. (2014). A potential link among antioxidant enzymes, histopathology and trace elements in canine visceral leishmanaiasis. International Journal of Experimental Pathology 95: 260-270.
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