Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News
Multiple-Antibiotic Resistance Pattern of Coliform Bacteria Isolated from Different Sources in Iwo, Nigeria
Current Issue
Volume 5, 2018
Issue 3 (September)
Pages: 41-45   |   Vol. 5, No. 3, September 2018   |   Follow on         
Paper in PDF Downloads: 36   Since Oct. 26, 2018 Views: 1027   Since Oct. 26, 2018
Adeleke Olufeyikemi Ajoke, Department of Biological Sciences, Faculty of Science, Bowen University, Iwo, Nigeria.
Owoseni Abimbola Adetokunboh, Department of Biological Sciences, Faculty of Science, Bowen University, Iwo, Nigeria.
Antibiotic resistance has become a big public health concern. Experiments were carried out to assess the antibiotic resistance pattern in coliform bacteria isolated from different sources in Iwo, Nigeria. Coliform bacteria were isolated on nutrient and xylose lysine deoxycholate (XLD) agar media and identified using standard procedures. Antibiotic multidisc containing ceftazidime (30µg), cefuroxime (30µg), gentamicin (10µg), ciprofloxacin (5µg), ofloxacin (5µg), augmentin (30µg), nitrofurantoin (300µg), and ampicillin (10µg) were used to study the resistance patterns of the isolates. Statistical analyses were carried out using the analysis of variance test (ANOVA) and the PAST (paleontology statistics) software analytical package where applicable. Twenty-five (25) Coliform bacteria consisting of four genera were isolated and identified. Enterobacter had the highest occurrence (44%) followed by Klebsiella (24%), E. coli (20%) while Citrobacter had the lowest occurrence (12%). Overall more Coliform isolates were recovered at the abattoir (8), cafeteria (6), poultry (5), cow ranch (4) and hostel sewage (2). The highest resistance observed by the isolates was to ampicillin (91%) followed by augmentin (55%), while many of the isolates were sensitive to ceftazidime, gentamicin, ofloxacin and ciprofloxacin. Polymerase chain reaction (PCR for 16S RNA) method was carried out to screen for CTX-M gene in beta-lactam resistant isolates. A number of organisms have developed multiple resistances to several antibiotics and of major concern are Coliforms in which new resistance characteristics have been discovered leading to increasing resistance to drug therapies. This may become a great public health concern if not curbed.
Coliforms, Antibiotic Resistance, CTX-M Gene, β-lactam Resistance, 16S RNA
Abiade-Paul, C., Kene, I. & Chah, K., (2005). Occurrence and antibiogram of Salmonellae in effluent from Nsukka Municipal abattoir. Nigerian Veterinary Journal, 27 (1): 48–53. Available at: http://www.ajol.info/index.php/nvj/article/view/3520/924 [Accessed August 3, 2016].
Alo, O. S. & Ojo, O. (2007). Use of antibiotics in food animals: A case study of a major veterinaryoutlet in Ekiti State, Nigeria. Nig. Vet. J. 28: 80-82.
Bartoloni, A., Pallecchi, L. & Benedetti, M. (2006). Multidrug resistant commensal Escherichia coli in children in Peru and Bolivia. Emerg Infect Dis 12: 907–913.
Benka-Coker, M. & Ojior, O., (1995). Effect of slaughterhouse wastes on the water quality of Ikpoba River, Nigeria. Bioresource Technology, 52: 5–12.
Boga, H. I., Okemo, P. O., Mwatha, W. E., Muthanga, J., Tsanuo, M. K., & Ikingura, J. R. (2007). Heavy metal tolerance and antibiotic resistance profiles of Gram-negative bacteria isolated from Lake Victoria, Kenya. J. Trop. Microbiol. Biotechnol. 3, 2.
Clinical and Laboratory Standards Institute (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA. 34 (1): 50-212.
Evans, H. L., Lefrak, S. N., Lyman, J. Smith, R. L., Chong, T. W., McElearney, S. T., Schulman, A. R., Hughes, M. G., Raymond, D. P., Pruett, T. L. & Sawyer, R. G (2007). Cost of gram-negative resistance. Crit Care Med 35: 89–95.
Fluckey, W. M., Loneragan, W. G., Warner, R., & Brashears, M. M. (2007). Antimicrobial drug resistance of Salmonella and Escherichia coli isolates from cattle feces, hides, and carcasses. Journal of Food Protection. 70: 551-556.
Gomez-Lus, R. (1998). Evolution of bacterial resistance to antibiotics during the last three decades. Int. Microbiol. 1: 279–284.
Gorman, R., Bloomfield, S. F. & Adley, C. (2002). A study of cross contamination of foodborne pathogens in the domestic kitchen in the Republic of Ireland. Int. J. Food Microbiol. 76: 143–150.
Kegode, R. B., Doetkott, D. K., Khaitsa, M. L. & Wesley, I. V. (2008). Occurrence of Campylobacter species, Salmonella species and generic Escherichia coli in meat products from retail outlets in the Fargo metropolitan area. J. Food Safety. 28: 111–125.
Knezevic, P., & Petrovic, O. (2008). Antibiotic resistance of commensal Escherichia coli of food-producing animals from three Vojvodinian farms, Serbia. International Journal of Antimi-crobial Agents. 31: 360-363.
Levy, S. B. (1997). Antibiotic resistance: an ecological imbalance in antibiotic resistance: Origins, Evolution, Selection and Spread, Wiley, Chichester (Ciba Foundation Symposium 207). Pp. 1-14.
Lewis, K., Hooper, D. & Ouillette, M. (1997). Microbial multidrug efflux pumps: new developments and clinical significance. ASM News 63, 605-10.
Mamza, S. A., Egwu, G. O. & Mshelia G. D. (2010). Antibiotic susceptibility patterns of betalactamase producing Escherichia coli and Staphylococcus aureus isolated from chickens in Maiduguri (Arid zone), Nigeria. Vet. Arhiv 80: 283-297.
Mattick, K., Durham, K, Domingue, G., Jorgensen, F., Sen, M., Schaffner, D. W. & Humphrey. T. (2003). The survival of foodborne pathogens during domestic washing-up and subsequent transfer onto washing-up sponges, kitchen surfaces and food. Int. J. Food Microbiol. 85: 213–226.
Miranda, J. M., Guarddon, M., Va´zquez, B. I., Fente, C. A. & Barros-Vela´zquez J. (2008). Antimicrobial resistance in Enterobacteriaceae strains isolated from organic chicken, conventional chicken and conventional turkey meat: a comparative survey. Food Control. 19: 412-416.
Mittal, G. S. (2004). Characterization of the Effluent Wastewater from Abattoirs for Land Application. Food Reviews International, 20 (3): 229–256. Available at: http://www.tandfonline.com/doi/abs/10.1081/FRI-200029422. 25th July, 2016.
Nwanta, J. A., Onunkwo, J. & Ezenduka, E. (2010). Analysis of Nsukka metropolitan abattoir solid waste and its bacterial contents in south eastern Nigeria: public health implication. Archives of environmental & occupational health, 65 (1): 21–6.
Nyamboya, R. A., Okemo, P. O., & Ombori, O. (2013). Isolation of High Antibiotic Resistant Fecal Bacteria Indicators, Salmonella and Vibrio Species from Raw Abattoirs Sewage in Peri-Urban Locations of Nairobi, Kenya. Greener Journal of Biological Sciences, 3 (5): 172–178.
Ojo, O. E., Ogunyinka, O. G., Agbaje, M., Okuboye, J. O., Kehinde, O. O., & Oyekun, M. A. (2012). Enterobacteriaceae isolated from free-range chickens in Abeokuta, Nigeria. vet. Arhiv 82: 577-589.
Owoseni A. & Onilude A. (2011) Antibiotic sensitivity and sequence amplification patterns of genes in multidrug resistant enterobacteria isolates from processed foods in some West African countries. Polish Journal of Microbiology 60 (4) 309-316.
Paterson, D. L. (2006). Resistance in Gram-negative bacteria: Enterobacteriaceae. The American Journal of Medicine. 119: 20–28.
Popęda, M., Płuciennik, E & Bednarek, A. K. (2014). Proteins in cancer resistance, Postępy Higieny I Medycyny Do´swiadczalnej, 68: 616–632.
Shea, K. M. (2004). Nontherapeutic use of antimicrobial agents in animal agriculture: Implications for pediatrics. Pediatrics 114: 862–868.
Singh, V. (2013). Antimicrobial resistance, in Microbial Pathogens and Strategies for Combating Them: Science, Technology and Education, Formatex Research Center. 1: 291–296.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
Copyright © 2013-, Open Science Publishers - All Rights Reserved