[1]
Ayomide Olubunmi Omojokun, Department of Microbiology, Federal University of Technology, Akure, Nigeria.
[2]
Anthony Okhonlaye Ojokoh, .
Improper indigestion is one of the major problems considered when blending legumes with carbohydrate foods. Therefore, this study was conducted to investigate the effects of fermentation and extrusion on the in-vitro protein and starch digestibilities of unripe plantain flour and cowpea flours blends. Three ratios of unripe plantain and cowpea flour were formulated. This was done as follows: 100:0, 80:20 and 60:40 in the ratio of unripe plantain flour to cowpea flour. The flour blends were fermented using solid state fermentation. Raw and fermented samples were further extruded, while raw flour blends served as the control. In-vitro protein and starch digestibilities of the flour blends were carried out using standard techniques. The results obtained revealed that fermentation and extrusion significantly increased the in-vitro starch digestibility of the flour blends with fermented extruded samples having the highest effect ranging from 49.62-52.67mg/100g and fermented unextruded samples having the least values (41.32- 46.91mg/100g). In-vitro protein digestibility was significantly increased with the raw samples having the least values (5.02-11.84mg/100g) and fermented extruded samples having the highest values (18.48-21.47mg/100g). The research revealed that fermentation and extrusion can be applied while formulating blends of legumes and carbohydrate foods in order to improve their digestibility alongside with the nutritional properties of the food.
[1]
Ojokoh, A. O. and Fagbemi, A. O. (2016). Effects of fermentation and extrusion on the proximate and organoleptic properties of cowpea-plantain flour blends. British Microbiology Research Journal, 13 (4): 1-13.
[2]
Olaoye, O. A., Onilade, A. A. and Idowu, O. A. (2006). Quality characteristics of bread produced from composite flours of wheat, plantain and soybeans. African Journal of Biotechnology. 5 (11): 1102-1106.
[3]
Otegbayo, B. O., Sobande, F. O. and Aina, J. O. (2002). Nutritional quality of soyplantain extruded snacks. Ecology of Food Nutrition. 42 (5): 473-474.
[4]
Mepba, D. H., Eboh, L. and Nwaojigwa, S. U. (2007). Chemical composition functional and baking properties of wheat-plantain composite flours. African Journal of Food Agriculture and Nutrition Development. 7 (1): 1-22.
[5]
Singh, B. B., Ajeigbe, H. A., Tarawali, S. A., Fernandez-Rivera, S. and Abubakar, M. (2003). Improving the production and utilization of cowpea as food and fodder. Fields Crops Research. 84 (1-2): 169-177.
[6]
Kasangi, D. M., Shitandi, A. A., Shalo, P. L. and Mbugua, S. K. (2010). Effect of spontaneous fermentation of cowpea leaves (Vigna unguiculata) on proximate composition, mineral content, chlorophyll content and beta-carotene content. International Food Research Journal. 17: 721-732.
[7]
Achi, O. K. (2005). Traditionally fermented protein condiments in Nigeria. African Journal of Biotechnology, 4 (13): 1612-1621.
[8]
Gabriel, R. A. O. and Akharaiyi, F. C. (2007). Effect of spontaneous fermentation on the chemical composition of thermally treated jack beans (Canavalia ensiformis L.). International Journal of Biological Chemistry. 1 (2): 91-97.
[9]
Jisha S., Sheriff J. T. and Padmaja G. (2010). Nutritional, functional and physical properties of extrudates from blends of cassava flour with cereal and legume flours. International Journal of Food Properties. 13 (5): 1002-1011.
[10]
Nwabueze, T. U., Iwe, M. O. and Akobundu, E. N. T. (2007). Unit operations and analyses for African breadfruit based spaghettitype products at extreme process combinations. Journal of Food Technology. 5 (1) 87-94.
[11]
Oluwole, O. B. and Olapade, A. A. (2011). Effect of extrusion cooking of white yam and bambara-nut blend on some selected extrudate parameters. Food Nutrition Sciences. 2 (6): 599-605.
[12]
Sobota, A. and Rzedzicki, Z. (2009). Effect of the extrusion process of corn semolina and pea hulls blends on chemical composition and selected physical properties of the extrudate. International Agrophysics. 23: 67-79.
[13]
Marcos, J. F., Antonion, G. S., Murillo, F. J., Dejani, L. A. and Jos, L. R. A. (2008). Effect of extrusion cooking in total carotenoid content on cream and orange flesh sweet potato cultivars. Hort. Bricultura Brasileira. http://dx.doi.org/10.1590/S0102-05362008000100022
[14]
Njoki, P. and Faller, J. F. (2001). Development of an extruded plantain/ corn/soy weaning food. International Journal of Food Science and Technology. 36: (4) 415- 423.
[15]
Iwe M. O. and Ngoddy P. O. (2000). Effect of extrusion on trypsin inhibitor contents of soy-sweet potato. Journal of Food Processing. 24: 453-463.
[16]
Zarzycki, P., Rzedzicki, Z. and Blaszczak, W. (2010). Application of oat whole-meal and protein components as modifiers of extrudates microstructure. International Agrophysics. 24: 397-406.
[17]
Chavan, U. D., Mc Kenzie, D. B. and Shaidi, F. (2001). Functional properties of protein isolates from beach pea (Lathyrus maritimus L.). Journal of Food Chemistry, 74: 177-187.
[18]
Englyst, H. N., Kingman, S. M. and Cummings, J. H. (1992). Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition, 46 (2): 33–50.
[19]
Raihanatu, M. B., Modu, S., Falmata, A. S., Shettima, Y. A. and Heman, M. (2011). Effect of processing (sprouting and fermentation) of five local varieties of sorghum on some biochemical parameters. Nigerian Society for Experimental Biology. (23) 2: 91 – 96.
[20]
Wedad, H. A., Abdullahi, H. E., Abdelmoneim, I. M. and Elfadil, E. B. (2008). Effect of fermentation, malt-pretreatment and cooking on antinutritional factors and protein digestibility of sorghum cultivars. Pakistan Journal of Nutrition 7 (2): 335-341, 2008.
[21]
Doudou, K. G., Taylor, J. R., Belton, P. S. and Hamaker, B. B. (2003). Factors affecting sorghum protein digestibility. Mini Review. J. Cereal Sci., 38: 117- 131.
[22]
Hassan, A. B., Isam A. M., Nuha M. O., Mohamed, M. E., Gammaa, A. O. and Elfadil, E. B. (2006). Effect of processing treatments followed by fermentation on protein content and digestibility of pearl millet (Pennisetum typhoideum) cultivars. Pakistan Journal of Nutrition. 5 (1): 86-89.
[23]
El Hag, M. E., Abdullahi, H. E. and Nabila, E. Y. (2002). Effect of fermentation and dehulling on starch, total polyphenols, phytic acid content and in vitro protein digestibility of pearl millet. Food Chemistry. 77: 193-196.
[24]
Mohiedeen, E., Tinay, A. H. E., Eikhalya, A. E. O., Babiker, E. E. and Mallasiy, L. O. (2010). Effect of fermentation on in-vitro protein digestibility, protein fractions and amino acids composition of maize (Zea mays Linnaus) cutlivars. Electronic Journal of Environmental Agriculture and Food Chemistry. 9: 838-847.
[25]
Olanipekun, B. F., Otunola, E. T. and Oyelade, O. J. (2015). Effect of fermentation on antinutritional factors and in-vitro protein digestibility of bambara nut (Voandzeia subterranean L.). Food Science and Quality Management. 39: 98-110.
[26]
Yagoub, A. E. and Abdalla, A. A. (2007). Effect of domestic processing methods on chemical composition, in-vitro digestibility of protein and starch and functional properties of bambara groundnut (Voandzeia subterranean) seed. Research Journal of Agriculture and Biolological Science, 3: 24-34.
[27]
Kiers, J. L., Van Laekan, A. E. A., Romboults, F. M. and Nout, M. J. R. (2000). In-vitro digestibility of Bacillus fermented soybean. International Food Microbiology, 60: 163-169.
[28]
Galal, O. A. A., Yufei, H., Moses, V. M. C. and Mohammed A. A. (2013). Functional properties and in-vitro protein digestibility of fermented sorghum and broad bean (Visia faba L. Major) blended flour. Pakistan Journal of Food Science. 23 (1): 10-16.
[29]
Singh, A., Yadav, N. and Sharma, S. (2012). Effect of fermentation on physicochemical properties & in vitro starch and protein digestibility of selected cereals. International Journal of Agriculture and Food Science. 2 (3): 66-70.
[30]
Elkhalifa, A. E., Schiffler, B. and Bernhard, R. (2004). Effect of fermentation on the starch digestibility, resistant starch and some physicochemical properties of sorghum flour. Nahrung. 48 (2): 91-4.