Micro Propagation of Banana from Male Flower of Banana Spike
[1]
Abdul Latheeff Mohammed Zuhry, Forest Department, Ministry of Mahaweli Development and Environment, Battaramulla, Sri Lanka.
[2]
Athambawa Mohamed Razmy, Department of Statistics, Sultan Qaboos University, Muscat, Sultanate of Oman.
Banana is one of the widely cultivated major fruit crop in many countries. Plants for large scale cultivations are obtained mainly through in-vitro micro propagation from apical meristem found in suckers as ex-plant. Easy contaminations and availability are the main issues to use suckers as ex-plant. A study was conducted to test the possibility of using male flowers of banana spikes commercially available in the market as ex-plant. Flowers of banana spikes obtained from green house under laboratory conditions were tested as ex-plants. Male flower’s hands and spike with male flowers were removed from these spikes and tested as ex-plants under two mediums MS+ 6.0 BAP mg/l and MS+3.0 mg/l BAP. It was found split spike with male flowers obtained from green house under laboratory conditions as ex-plant gave a good growth in in-vitro propagation under the medium MS+ 6.0 mg/l BAP. Medium MS+3.0 mg/l BAP is not providing any growth and male flower hands alone also didn’t provide any growth in any medium. This new propagation method that split spike with male flowers as ex-plant in MS+ 6.0 mg/l BAP medium was tested in commercially available local banana verities in Sri Lanka. A good growth and multiplication rates were observed in the local varieties of Rathambala, Seeni and Ambul. Therefore this method could be a significant breakthrough in banana micro propagation in Sri Lanka since it has many advantages over use of apical meristem such as easy to acquire, easy transportation, cost effective, minimum harm to the mother plant, easy sterilization, less harm to environment, low contamination rate and restricted movement of soil borne pathogens.
Banana, Ex-Plant, In-Vitro-Propagation, Male Flower
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