Effect of Toothpaste on Oral Microbial Flora (Bacteria and Fungi)
[1]
Obiazi Helen A. K., Department of Microbiology, Ambrose Alli University, Ekpoma, Nigeria.
[2]
Dic-Ijiewere Ebenezer Oseremen, Department of Chemical Pathology, Ambrose Alli University, Ekpoma, Nigeria.
This study was carried out to evaluate the effect of toothpaste on oral microbial flora (bacteria and fungi) isolated of students of Ambrose Alli University, Ekpoma. The sample size of the study consisted of 50 subjects from Ekpoma, randomly selected across Ekpoma, consisting of 20 males and 30 females. Sterile swab sticks were used to collect samples from the oral cavity (tongue, teeth and the inner cheek) aseptically from each subject using swab sticks. The organisms isolated were Bacteria: Streptococcus mutans (25), Staphylococcus aureus (10), Escherichia coli (2), Klebsiella specie (5), and Fungi: Candida albicans (4) and Rhizopus spp (2). The antibiotics sensitivity indicated Close-Up Deep action (Red hot) and Macleans (Fresh mint) were sensitive to all the organisms isolated, Pepsodent - Natural white (Gel), Oral – B (Pro-Health), Close-Up Herbal and Colgate were sensitive to three (3) organisms, while the antibiotics sensitivity of the oral fungal flora indicated Close-Up Deep action (Red hot), Macleans (Fresh mint) and Oral – B (Pro-Health) showed sensitivity to all the fungal isolates while Pepsodent - Natural white (Gel), Close-Up Herbal and Colgate were sensitive to one (1) of the fungal isolate. Analysis of the inhibition of bacteria by the toothpastes studied shows that they are effective for some bacterial species than others. However, the microbial quality of toothpaste formulations marketed in the open markets has met the drug standard which excludes the presence of the index, indicator or pathogenic organism in drugs to be consumed by the populace.
Toothpaste, Teeth, Microbial Flora, Oral, Students
[1]
Arweiler, N. B., Auschill, T. M., Reich, E. and Netuschil, L. (2002). Substantivity of toothpaste slurries and their effect on reestablishment of the dental biofilm. J ClinPeriodontol. 29: 615-621.
[2]
Davies, R. M. (2008). Toothpaste in the control of plaque/gingivitis and periodontitis. Periodontol. 48: 23-30.
[3]
Marsh, P. D. (2010). Controlling the oral biofilm with antimicrobials. J Dent. 38 Suppl 1: S11-S15.
[4]
Prasanth, M. (2011). Antimicrobial efficacy of different toothpastes and mouth rinses: an in vitro study. Dent Res J Isfahan. 8: 85-94.
[5]
Maltz, M. and Beighton, D. (2012). Multidisciplinary research agenda for novel antimicrobial agents for caries prevention and treatment. Adv Dent Res. 24: 133-136.
[6]
Manupati, P. (2011). Antimicrobial Efficacy of Different Toothpastes and Mouth rinses: An in vitro Study. Dent, Res. J. (Isfahan). 8 (2): 85–94.
[7]
William, M. I. and Cummuns, D. M. (2003). The Technology behind close-up total advanced fresh, comprehensive continue education on dentals, Brit. J. of Dent. Surg. 24 (5): 4-9.
[8]
Mohan, K. K. P., Priya, N. K. and Madhushankari, G. S. (2013). Anti-Cariogenic Efficacy of Herbal and Conventional Toothpastes - A Comparative In-Vitro Study, J. of Int. Oral Health. 5 (2): 8-13.
[9]
Lee, S. S., Zhang, W. and Li, Y. (2004). The antimicrobial potential of 14 natural herbal dentifrices: results of an in vitro diffusion method study. J Am Dent Assoc. 135: 1133-1141.
[10]
Netuschil, L., Brexc, M., Heumann, C. and Hoffman, T. (2005). Clinically controlled 6- month study of the influence of toothpastes with anti-inflammatory ingredients on plaque and gingivitis. Quintessenz. 56: 1277–1286.
[11]
Ozaki, F., Pannuti, C. M., Imbronito, A. V., Pessotti, W., Saraiva, L. and Maria de Freitas, N. (2006). Efficacy of a herbal toothpaste on patients with established gingivitis - a randomized controlled trial. Braz Oral Res. 20: 172–177.
[12]
Peck, M. T., Africa, C. W. J., Stephen, L. X. G., Marnewick, J. M. and Abdul, M. (2011). An in-vitro analysis of the antimicrobial efficacy of herbal toothpastes on selected primary plaque colonizers. Int Journal of Clinical Dental Science. 2 (3): 28–32.
[13]
World Gazetteer (2007): Population of Cities, news, divisions. htt://world gazetteer.com/ng.php.
[14]
Monica Cheesbrough. (2005). Discrete Laboratory Practice in Tropical Countries Part 1, Cambridge Second Editions. Published by Press Syndicate of the University of Cambridge.
[15]
Asmara, W., Astuti, I., Tandelilin, R. T. C. and Jonarta, A. L. (2010). Systemic IL-1β and TNF-α production of E. coli Lipopolysaccharide-Induced Periodontitis model on rats. The Indonesian Journal of Dentistry. 1 (1): 49-54.
[16]
Gamboa, F., Estupinan, M. and Galindo, A. (2004). Presence of Streptococcus mutans in saliva and its relationship with dental caries: Antimicrobial susceptibility of the isolates. Universitas Scientiarum. 9 (2): 23–27.
[17]
Hoikhian, C. S. O. and Okoror, L. O. (2012). Resistance of oral bacterial species to varied toothpaste effects, Int. J. of Eng. Res. and Sci. and Tech. 1 (1): 1-10.
[18]
Nwankwo, I. U. and Ihesiulo, S. C. (2014). Comparative Analysis of the Antibacterial Potentials of Some Brands of Toothpaste Commonly Used In Umuahia, Abia State. J. of Pharm. and Bio. Sci. (IOSR-JPBS). 9 (3): 50-54.
[19]
Bou-Chacra, N. A., Gobi, S. S., Ohara, M. T. and Pinto, T. J. A. (2005). Antimicrobial activity of four different dental gel formulas on cariogenic bacteria evaluated using the liner regression method. Revista Brasileira De Ciencias Farmaceuticas. 41 (3): 323–331.
[20]
Jenkins, S., Addy, M. and Newcombe, R. G. (1994). Dose response of chlorhexidine against plaque and comparison with triclosan. J Clin Periodontol. 21 (4): 250–255.
[21]
Harper, P. R., Milsom, S., Wade, W., Addy, M., Moran, J. and Newcombe, R. G. (1995). An approach to efficacy screening of mouthrinses: studies on a group of French products (II). Inhibition of salivary bacteria and plaque in vivo. J Clin Periodontol. 22 (9): 723-727.
[22]
Larone, D. H. (2002). Identification of fungi in culture, In: A guide to identification of medically important fungi fourth Edition, ASM Press, Washington DC; Pp. 229-253.
[23]
Okpalugo, J., Ibrahim, K. and Inyang, U. S. (2009). Toothpaste formulation efficacy in reducing oral flora, Trop. J. of Pharm. Res. 8 (1): 71-77.
[24]
Saravia, M. E., Nelson-Filho, P., Silva, R. A., De Rossi, A., Faria, G. and Silva, L. A. (2013). Recovery of mutans streptococci on MSB, SB-20 and SB-20M agar media. Arch Oral Biol. 58: 311-316.
