Screening of <i>Moringa Oleifera</i> Leaf Extract on Various Human Cancerious Cell Line Using Microtiter Plate Based Assay

Vipul Prahladbhai Patel; Vishal Vijay Pande; Dhanashri Devendra Borawake

doi:7560146

The Moringa plant (Moringa oleifera) has been the object of much research due to its multiple uses and well-known medicinal properties. It has been reported that leaf extract of Moringa oleifera has anti-proliferative effect on the human cervical cancer cell line, KB cells and alveolar cancer cell line A549 cells. The object of the present work is to investigate the anti-cancer effect of Moringa oleifera leaf extract on various human cancerious cells like DU-145, MCF-7, HEP-3B, K-562 and HCT-15. Cold extraction of powdered leaves was carried out using hydro-alcoholic mixture (water: methanol; 30:70 v/v). Cell viability was measured by MTT assay and IC₅₀ value was calculated using Graph Pad Prism Ver. 6.01 and Microsoft word excel 2008 at one third dilutions with ten different points which was compared with standard anticancer drug Colchicin. The effect of the extract on VERO (normal) cell was evaluated as well. The leaf extract showed a dose-dependent inhibition on cell proliferation K-562 (IC₅₀ value 32.43 µg/ml, R²=0.9253), DU-145 (IC₅₀ value 42.74 µg/ml, R²=0.9862), HCT-15 (IC₅₀ value 5.213 µg/ml, R²=0.9647), MCF-7 (IC₅₀ value 24.76 µg/ml, R²=0.9701), HEP-3B (IC₅₀ value 29.37 µg/ml, R²=0.9772). It showed low cytotoxicity in the normal cells. Results of DNA fragmentation study showed significant fragmentation pattern in case of K-562, DU-145 and HCT-15 cells when treated by IC50 concentration. The present study suggests that the hydro-alcoholic leaf extract of M. Oleifera induces anticancer effect on K-562, DU-145 and HCT-15 cancer cells.

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