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Glucosinolates Detoxification by Solid State Fermentation as Fungi "Biological" and Copper Sulfate Solution "Chemical" Methods
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Volume 2, 2017
Issue 6 (December)
Pages: 41-53   |   Vol. 2, No. 6, December 2017   |   Follow on         
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Husain Abd Allah El-Fadaly, Agriculture Microbiology Department, Faculty of Agriculture, Damietta University, Damietta City, Egypt.
Sherif Mohamed El-Kadi, Agriculture Microbiology Department, Faculty of Agriculture, Damietta University, Damietta City, Egypt.
Mostafa Maher El-Moghazy, Animal Production Department, Faculty of Agriculture, Damietta University, Damietta City, Egypt.
Ahmed Ali Soliman, Animal Nutrition Research Department, Animal Production Research Institute (APRI), Agricultural Research Centre (ARC), Dokki, Giza City, Egypt.
Mahmoud Salama Mahmoud El-Haysha, Animal Nutrition Research Department, Animal Production Research Institute (APRI), Agricultural Research Centre (ARC), Dokki, Giza City, Egypt.
The seeds of Brassicaceae or Cruciferae family contain secondary plant products with various biological effects, including beneficial and harmful ones. These products contain glucosinolates (Gls). They have many types, but the bitter taste type and the anti-nutritive effect are thioglucosides, which contain a sulfate group on the aromatic ring. The seeds of the watercress plant, which were used in the current study in order to try to get rid of the impact of those substances, by cracking by the enzyme myrosinase and is able to crack and decompose Glycoside bond, a water enzyme, and Using six fungal strains: Sclerotinia sclerotiorum, Macrophomina phaseolina, Trichoderma longibrachiatum, Penicillium digitatum, and Fusarium oxysporum in addition to one chemical treatment using hydrothermal copper sulphate (CuSO4), and comparing the previous seven fungal and chemical biological parameters with a control treatment in which the parasite was treated The seeds of watercress are the same as the pre-treatment in the preparation of the solid fermentation environment but without fertilization of the environment in any fungal chain and the control treatment was incubated in the same conditions as the six fungal treatments. Because the seeds of watercress seeds are rich in antioxidants and anti-cancer, These materials are in all experimental and knowledgeable transactions The following tests were performed: Total polyphenols, Total flavonoids, Reducing power, ABTS+ action radical, DPPH radical, and Hydrogen peroxide scavenging activity, in addition to analysis The results of the various chemical treatments from the normal chemical analysis of protein, fiber, ash, fat and carbohydrates as well as the major mineral elements, to determine the effect of each treatment on the seedling of watercress seeds (Eruca sativa meal). The biological treatment included natural thermal treatment by sterilization of the plant material and biological fungal treatment by pollination and incubation and then the secretion of the water enzyme Capable of cracking glucosinolate for other substances. The results of this study showed that there are three biological factors which are T2 (Macrophomina phaseolina), T4 (Trichoderma longibrachiatum), and T6 (Penicillium digitatum) achieved the highest ratios that exceed the control treatment (Non-biologically and chemically treated), in the measurements of total polyphenols and total flavonoids content, Reducing power, (ABTS+) action radical, (DPPH) radical, and Hydrogen peroxide (H2O2) scavenging activity of rocket meal seed (RMS) in different treatments.
Solid Fermentation, Glucosinolates Detoxification, Copper Sulfate Solution, Antifungal Activity, Isothiocyanates, Antioxidant Activity, Chemical Composition, Watercress Plant (Rocket Seed Meal "RSM")
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