Biological Activity of Eugenol Acetate as Antibacterial and Antioxidant Agent, Isolation from Myrtus communis L. Essential Oil
The fundamental goals of this study is to determine the chemical composition of Myrtus communis (M. communis) essential oil the local and to assess its antimicrobial activity against Esherichia coli, Proteus mirabilis isolated from urine, Staphylococcus aureus from Ear, Pseudomonas aeruginosa and Acinetobacter sp. from burns and Salmonella typhi from blood. Column Chromatography, Thin Layer Chromatography (TLC), Infra (IR) Red, Nuclear Magnetic Resonance Spectroscopy (H1NMR) and Gas chromatography-mass spectrometry (GC-MSS) were used to determine the chemical composition of essential oil from M. communis leaves. A disk diffusion method was applied to evaluate the antibacterial activity and microdilution susceptibility assay method was utilized to evaluate the (minimum inhibitory concentration) (MIC) and antioxidant activity was analyzed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays. Column Chromatography, (TLC), (IR), (H1NMR) and (GC-MS) revealed that M. communis contained eugenol acetate compound. The results showed broad antibacterial activity against pathogenic bacteria isolates tested with ranged between (13.5-36.5) mm except P. aeruginosa and less minimum inhibitory concentration ranged between 25-100 µg\ml. The antioxidant activities have shown high rates of inhibition. A verification of non-toxicity of eugenol acetate compound against human blood revealed a negative test.
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