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Biological Activity of Eugenol Acetate as Antibacterial and Antioxidant ‎Agent, Isolation from Myrtus communis L. Essential Oil
Current Issue
Volume 1, 2016
Issue 2 (August)
Pages: 6-11   |   Vol. 1, No. 2, August 2016   |   Follow on         
Paper in PDF Downloads: 64   Since Sep. 13, 2016 Views: 1179   Since Sep. 13, 2016
Authors
[1]
Rashid Rahim Hateet, Department of Biology, College of Sciences, University of Maysan, Maysan, Iraq.
[2]
Ahmad Khadhim Hachim, Department of Biology, College of Education for Pure Sciences, University of Basra, Basra, Iraq.
[3]
Hassan Shawi, Department of Bacteriology, Al-Sadder Teaching Hospital, Maysan, Iraq.
Abstract
The fundamental goals of this study is to determine the chemical composition of Myrtus communis (M. communis) essential oil the local and to assess its antimicrobial activity against Esherichia coli, Proteus mirabilis isolated from urine, Staphylococcus aureus from Ear, Pseudomonas aeruginosa and Acinetobacter sp. from burns and Salmonella typhi from blood. Column Chromatography, Thin Layer Chromatography (TLC), Infra (IR) Red, Nuclear Magnetic Resonance Spectroscopy (H1NMR) and Gas chromatography-mass spectrometry (GC-MSS) were used to determine the chemical composition of essential oil from M. communis leaves. A disk diffusion method was applied to evaluate the antibacterial activity and microdilution susceptibility assay method was utilized to evaluate the (minimum inhibitory concentration) (MIC) and antioxidant activity was analyzed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays. Column Chromatography, (TLC), (IR), (H1NMR) and (GC-MS) revealed that M. communis contained eugenol acetate compound. The results showed broad antibacterial activity against pathogenic bacteria isolates tested with ranged between (13.5-36.5) mm except P. aeruginosa and less minimum inhibitory concentration ranged between 25-100 µg\ml. The antioxidant activities have shown high rates of inhibition. A verification of non-toxicity of eugenol acetate compound against human blood revealed a negative test.
Keywords
Myrtus communis L, Essential Oil, Antibacterial, Antioxidant, Pathogenic Bacteria
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