Invitro Antibacterial Activity of <i>Muricata L.</i> Leaves Extract on Some Bacteria Isolates

Obiazi Helen Akinagwu Kuhodu; Dic-Ijiewere Ebenezer Oseremen; Amhanre Idi Napoleon; Aitufe Felix Okoro; Igwe Reginald Marvin Ndubuisi

doi:7550145

Annona Muricata L. or infamously known as soursop is one of Malaysian exotic fruits from the family Annonaceae which has been shown to have medicinal properties. The study was carried out to evaluate the invitro antibacterial activity of A. muricata L. leaves extract on organisms isolated from Urine, Stool and wound of patients. Aqueous decoctions of the leaves were prepared by adding 10 grams of Soursop leaf in 100ml sterile distilled water and boiled over a low flame for 15 minutes. After cooling, the contents of the flasks were filtered with double filter paper and sterile filters to remove impurities and concentrations of 5% and 10%of extract were prepared using sterile distilled water. The minimum inhibitory concentration gave the lowest concentration of the aqueous extract that can inhibit the growth of the bacteria isolates. The percentage of bacterial isolate to various concentrations of soursop fruits and juice extracts in which 50% concentration/dilution of fruit extracts recorded the highest of 14(93.3%) with E. coli, Salmonella spp. and shigella was 10(100%), 2(50%) and 0(0%) respectively. However, the 10% of fruits extracts was second with 12(80%) and Escherichia coli recorded the highest of 10(100%) followed by Salmonella spp least of 2(50%) while absent in shigella. As for 50% Juice extracts, a total of 2(13.3%) was recorded with Salmonella spp and shigella with 1(100%) respectively. While for 10% juice extracts, only Shigella of 1(100%) was recorded. The present study demonstrated the in-vitro efficacy of Soursop fruits extracts which was highest against E. coli followed by Salmonella spp. Hence, this study proves to an extent that the Soursop fruits extract when used against microbiota has sufficient anti-microbial property.

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