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Effects of Co-Administration of Folic Acid and/or Magnesium on Biomarkers of Oxidative Stress in Streptozotocin- Induced Diabetic Wistar Rat
Current Issue
Volume 3, 2018
Issue 2 (March)
Pages: 16-20   |   Vol. 3, No. 2, March 2018   |   Follow on         
Paper in PDF Downloads: 37   Since Jul. 2, 2018 Views: 1021   Since Jul. 2, 2018
Authors
[1]
Goji Anthony Donatus Teru, Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medicine Kaduna State University, Kaduna, Nigeria.
[2]
Mohammed Aliyu, Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, Ahmadu Bello University, Zaria, Nigeria.
[3]
Kawu Mohammed Umar, Department Veternary Physiology, Ahmadu Bello University, Zaria, Nigeria.
[4]
Isah Anthony Denis, Department of Obstetrics and Gynaecology University of Abuja Teaching Hospital, Abuja, Nigeria.
Abstract
Diabetes mellitus (DM) is a global public health problem with increasing prevalence. It is a chronic disorder characterized by hyperglycemia and the late development of vascular and neuropathic complications. This work was designed to study the Effects of Co-administration of Folic Acid and/or Magnesium on Biomarkers of Oxidative Stress in Streptozotocin- Induced Diabetic Wistar Rat. Healthy albino rats weighing between 150g and 200g were used. The rats were randomly allotted into six groups, each containing five albino rats respectively. Five of the groups (II, III IV V and VI) were induced with diabetes by single intraperitoneal (i.p) injection of freshly prepared in 0.1 mol/L citrate buffered solution (pH 4.5) of streptozotocin (Sigma Aldrich, St. Louis, MO, USA) at a dose of 60 mg/kg body weight. Control (vehicle) rats were injected with equal volume of 0.1 mol/L citrate buffer. Four days after STZ injection, diabetes induction was confirmed by measuring fasting blood glucose level in a tail vein blood samples using ACCU-CHEK compact plus glucometer (Roche, France). Rats with glucose level of 200 mg/dl or higher were considered as diabetic. After the induction of diabetes the rats were treated using the FA and Mg separately and in combination respectively according to group daily, whereas, the other group (I) was not given any treatment and this served as the normal control, providing a baseline data. The results of the present study indicated significant aberrations in the lipid profile markers associated with carbohydrate, protein and lipid metabolisms in STZ induced-Type I diabetic The diabetic groups showed increased levels of malondialdehyde (MDA), reduction in the level of antioxidant defense status, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) to counteract the diabetes associated oxidative damaged was also well evident. These anomalies were all ameliorated to about normal values after four weeks of treatment with FA+Mg. This suggests the synergistic beneficial effects of folic acid and Magnesium against STZ-induced diabetes in Wistar Rats.
Keywords
Diabetes Mellitus, Streptozotocin, Folic Acid, Wistar Rats
Reference
[1]
NCD Risk factor Collaboration. (2016). Worldwide trend in diabetes since 1980: a pooled analysis of 751 population-based studies with 4.4 million participants. Lancet, 387: 1513-30.
[2]
Benjamin, M. L and Thomas, M. M. (2015). Diabetes and Cardiovascular disease: epidemiology, biologica mechanisms, treatment recommendation and future research. World Journal Diabetes 6 (130): 1246-1258.
[3]
Fasannmade, O. A and Jack, D. S. (2015). Daibetes Care in Nigeria. Annals of Global Health 81 (6): 822.
[4]
Firdous, S. M. and Koneri, R. (2014). Antidiabetic and antioxidant activities of Ipomoea staphylina Leaves in STZ-induced diabetic mice. Journal of Pharmaceutical Science Technology, 3: 2-5.
[5]
Paget, G. E. and Barnes, J. M. (1964). Toxicity test. In: Laurence DR, Bacharach AL, editors. Evaluation of drug activities pharmacometrics. London and New York: Academic Press; pp. 134–166.
[6]
Hfaiedh, N., Murat, J. C. and Elfeki, A. (2013). Diabetes-induced damages in rat kidney and brain protective effects of natural antioxidants. Journal of Nutrition and Food Science, 3: 4-6.
[7]
Stanley, P., Mainzen, P. and Venugopal, M. P. (2001). Anti-oxidant action of Tinospora, Cordifolia root extract in alloxan-induced diabetic rats. Biotechnology and Applied Biochemistry, 15: 20-31.
[8]
Martin, J. P., Dailey, M. and Sugarman, E. (1987). Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Archives of Biochemistry and Biophysics, 255: 329-336.
[9]
Beer, R. F. and Sizer, I. W. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195: 133-140.
[10]
Takebe, G. (2002). A comparative study on the hydroperoxide and thiol specificity of the glutathione peroxidase family and selenoprotein. Journal of Biological Chemistry, 277 (43): 41254-41258.
[11]
Janero, D. R. (1990). Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free-Radical Biology and Medicine, 9: 515-540.
[12]
Duncan, R. C., Knapp. R. G., and Miller, M. C. 1977. “Test of Hypotheses in Population Means” In IntroductoryBiostatistics for Health Science, N. Y.: John Wiley and SonInc, 71-6.
[13]
Maryam, C., Mohammad, H. J., Zahra, S., Saeed, H., Mahmoud, D. (2014). Assessment of range of malonyldialdehyde in serum and some anti-oxidant enzymes in patients with diabetes type-2 with controlled blood sugar in comparison with the diabetics with uncontrolled blood sugar. Advances in Environmental Biology, 8 (9): 18-22.
[14]
Gopalakrishnan, G. and Dhanapal, C. K. (2014). Evaluation of in-vivo antioxidant activity of methanolic extract of coleus ettiveroides Jacob in streptozotocin-induced oxidative stress in rats. International Journal of Pharmacy and Pharmaceutical Science, 6 (1): 590-592.
[15]
Ceretta, L. B., Reus, G. Z., Abelaira, H. M., Ribeiro, K. F., Zappellini, G., Felisbino, F. F., Steckert, A. V., Dal-Pizzol, F. and Quevedo, J. (2012). Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan-induced diabetic rats. Experimental. Diabetes Research, 8: 2012-2022.
[16]
Jonah, S. A. (2016). Pharmacology of free radicals and the impact of reactive oxygen species on the testis. Journal of Reprod Infertil 14 (4): 158-172.
[17]
Maher, M. A. (2014). Combined therapy of rutin and silymarin has more protective effects on streptozotocin-induced oxidative stress in rats. Journal of Applied Pharmaceutical Science, 4 (1): 21-28.
[18]
Malgorzata, N and Andrzej, G. (2016). The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related disease and other pathologies of the anterior and posterior segment in adult. Oxid med Cell Longev. 2016: 3164734.
[19]
Peter, P., Janka, V., Pavol, S. Jr., Ladislav, V and Pavol, S. (2016). Reactive oxygen species and antioxidant defense in human gastrointestinal disease. Integrative Med. Research. 5 (4): 250-258.
[20]
Sağlam, O., Değirmenci, I., Cengiz, M. U. and Güneş, V. (2012). The protective effects of cinnamon and sugar tea extract on diabetic rats with interrelationships between oxidative stress and DNA damage. African Journal of Pharmacy and Pharmacology, 6 (43): 3012-3017.
[21]
Charinya, P., Waranya, C. and Kanokwan, J. (2014). Improvement of antioxidant balance in type-1 diabetes mellitus mice by glutathione supplement. Pakistan Journal of Pharmaceutical Science, 27 (6): 1731-1737.
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