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Assessment of the Microbiological Quality and Efficacy of Two Common Disinfectants Used in Hospital Laboratory
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Volume 2, 2017
Issue 6 (November)
Pages: 31-43   |   Vol. 2, No. 6, November 2017   |   Follow on         
Paper in PDF Downloads: 33   Since Jan. 15, 2018 Views: 1085   Since Jan. 15, 2018
Enitan Seyi Samson, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.
Ochei John Okeleke, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.
Digban Kester Awharentomah, Department of Medical Laboratory Science, Igbinedion University, Okada, Nigeria.
Akele Yomi Richard, Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Nigeria.
Nwankwo Kasie Josephine, Department of Medical Laboratory Science, Igbinedion University, Okada, Nigeria.
Arisi Chinedu Paul, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.
The present study assessed the microbiological quality and efficacy of two common disinfectants (Jik and Lysol) used in a hospital laboratory. Sterility test using Nutrient Agar and Sabour Dextrose Agar plates incubated at 37°C and 25°C, respectively, were employed to detect the present of potential bacterial and fungal contaminants in 3 new batches of stock disinfectants. Swabs of work-bench surfaces designated as Site 1, 2 and 3 were collected in triplicate at the end of each business day (i.e, before disinfection) and also after disinfection with 30% Jik and 2.5% Lysol dilution and cultured in tubes containing 3 ml of Tryptic Soy Broth medium and 0.1 mL Neutralizer. Surface viable count was carried out to determine the bacterial population density of three sites pre-disinfection and post-disinfection. Colonies of bacteria were identified by Gram-stain, motility test and routine biochemical tests. The efficacy of the disinfectants against each bacterial isolate at 10 min contact time was determined using the quantitative suspension test. The killing rate of the disinfectants was expressed by plotting the logarithms of surviving cells (CFU/mL) against exposure time (min) of the disinfectant. The outcome of the study showed that the microbiological quality of the two disinfectants tested was satisfactory. Bacterial distribution pre-disinfection include: Staphylococcus epidermidis, Enterococcus aerogenes, Proteus mirabilis, Bacillus subtilis, Pseudomonas aeruginosa and Klebsiellia pneumoniae; while only B. subtilis, P. aeruginosa and K. pneumoniae were recovered post-disinfection. Lysol proved to be more potent than Jik at the dilution and contact time tested with a log reduction of bacterial population ≥5. S. epidermidis, E. aerogenes and P. mirabilis were completely killed by the two disinfectants within the 10 minutes contact time. While, a population density of 1-2 log CFU/ml of B. subtilis, P. aeruginosa and K. pneumoniae still survived after 10 min exposure to Lysol and Jik. The outcome of this study further strengthening earlier works and underscored the need to periodically assess the microbiological quality and efficacy of disinfectants routinely supplied to the laboratory to ensure proper control of infections by using right disinfectant in right concentration for a right contact time.
Hospital Laboratory, Disinfection, Disinfectants, Jik, Lysol, Quality, Efficacy
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