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Assessment of the Microbiological Quality and Efficacy of Two Common Disinfectants Used in Hospital Laboratory
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Volume 2, 2017
Issue 6 (November)
Pages: 31-43   |   Vol. 2, No. 6, November 2017   |   Follow on         
Paper in PDF Downloads: 28   Since Jan. 15, 2018 Views: 585   Since Jan. 15, 2018
Enitan Seyi Samson, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.
Ochei John Okeleke, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.
Digban Kester Awharentomah, Department of Medical Laboratory Science, Igbinedion University, Okada, Nigeria.
Akele Yomi Richard, Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Nigeria.
Nwankwo Kasie Josephine, Department of Medical Laboratory Science, Igbinedion University, Okada, Nigeria.
Arisi Chinedu Paul, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.
The present study assessed the microbiological quality and efficacy of two common disinfectants (Jik and Lysol) used in a hospital laboratory. Sterility test using Nutrient Agar and Sabour Dextrose Agar plates incubated at 37°C and 25°C, respectively, were employed to detect the present of potential bacterial and fungal contaminants in 3 new batches of stock disinfectants. Swabs of work-bench surfaces designated as Site 1, 2 and 3 were collected in triplicate at the end of each business day (i.e, before disinfection) and also after disinfection with 30% Jik and 2.5% Lysol dilution and cultured in tubes containing 3 ml of Tryptic Soy Broth medium and 0.1 mL Neutralizer. Surface viable count was carried out to determine the bacterial population density of three sites pre-disinfection and post-disinfection. Colonies of bacteria were identified by Gram-stain, motility test and routine biochemical tests. The efficacy of the disinfectants against each bacterial isolate at 10 min contact time was determined using the quantitative suspension test. The killing rate of the disinfectants was expressed by plotting the logarithms of surviving cells (CFU/mL) against exposure time (min) of the disinfectant. The outcome of the study showed that the microbiological quality of the two disinfectants tested was satisfactory. Bacterial distribution pre-disinfection include: Staphylococcus epidermidis, Enterococcus aerogenes, Proteus mirabilis, Bacillus subtilis, Pseudomonas aeruginosa and Klebsiellia pneumoniae; while only B. subtilis, P. aeruginosa and K. pneumoniae were recovered post-disinfection. Lysol proved to be more potent than Jik at the dilution and contact time tested with a log reduction of bacterial population ≥5. S. epidermidis, E. aerogenes and P. mirabilis were completely killed by the two disinfectants within the 10 minutes contact time. While, a population density of 1-2 log CFU/ml of B. subtilis, P. aeruginosa and K. pneumoniae still survived after 10 min exposure to Lysol and Jik. The outcome of this study further strengthening earlier works and underscored the need to periodically assess the microbiological quality and efficacy of disinfectants routinely supplied to the laboratory to ensure proper control of infections by using right disinfectant in right concentration for a right contact time.
Hospital Laboratory, Disinfection, Disinfectants, Jik, Lysol, Quality, Efficacy
AHRQ. Ending Health Care-Associated Infections. Available at: www.ahrq.gov/research/findings/factsheets/errors-safety/haicusp/index.html. 2013.
Ducel, G. Guide pratique pour la lutte contre l’infection hospitalière. 1995; WHO/BAC/79.1.
Benenson, A. S. Control of communicable diseases manual (16th edition), American Public Health Association, Washington, DC, USA. 1995.
McDonald, L. C., Jarvis, W. R. Linking antimicrobial use to nosocomial infections: the role of a combined laboratory epidemiology approach. Ann. Intern. Med., 1998; 129: 245-247.
Capretti, M., Sandri, F., Tridapalli, E., Galletti, S., Petracci, E., Faldella, G. Impact of a standardized hand hygiene program on the incidence of nosocomial infection in very low birth weight infants. Am. J. Infect. Cont., 2008; 36 (6): 430-435.
Piskin, N., Celebi, G., Kulah, C., Mengeloglu, Z., Yumusak, M. Activity of a dry mist-generated hydrogen peroxide disinfection system against methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii, Am J Infect Control., 2011; 39 (9): 757-762.
Guimaraes, M. A., Tibana, A., Nunes, M. P., Santos, K. R. Disinfectant and antibiotic activities: a comparative analysis in Brazilian hospital bacterial isolates. Braz. J. Microbiol., 2000; 31 (1): 193-199.
UNC. Principle of sterilization and disinfection, Environment health and safety, Chapel Hill, North Carolina, USA. 2017.
Singh, M., Sharma, R., Gupta, p. k., Sharma, M., Taneja N. Comparative efficacy evaluation of disinfectants routinely used in hospital practice. Indian J. Crit Care Med., 2012; 16 (3): 123-129.
Theraud, M., Bedovin, Y., Guiguen, C., Gangneux, J. P. Efficacy of antiseptics and disinfectants on clinical and environmental yeast isolates in planktonic and biofilm conditions. J. Med. Microbiology, 2004; 53: 1013-1015.
CDC. Healthcare-associated Infections (HAIs). Centre for Disease Control. Available from: http://www.cdc.gov/HAI/surveillance.2015.
Rutala, W. A., Weber, D. J. The benefits of surface disinfection. Am J Infect Control; 2004; 32 (4): 226-231.
Yousefi, M. R., Nazari, M., Samarghandi, M. R., Shams, M. Evaluation of efficacy of the current disinfectants on Staphylococcus epidermidis and Pseudomonas aeroginosa isolated from hospitals of Hamadan. Zahedan J. Res Med Sci., 2007; 8 (4): 287-297.
OSHC. Chemical Safety in the Workplace: Guidance Notes on Safe Use of Chemical Disinfectants. Occupational Safety and Health Branch Labour Department, Occupation Safety and Health Council. Hong Kong. 2007; pp. 1-33.
Cheesbrough, M. “Decontamination of infectious material and disposal of laboratory waste.” In: Cheesbrough, M. (ed.). District Laboratory Practice in Topical Countries, Part 1. Cambridge University Press, Cape Town, South Africa. 2006a; pp. 67-70.
Ochei, J. O., Kolhatkar, A. A. Chemical sterilization (disinfectants and Antiseptics) In: Ochei, J. O. and Kolhatkar, A. A. (eds.). Medical Laboratory Science: Theory and Practice, Tata McGrew-Hill, New Delhi, India. 2007a; pp. 553-556.
Gajadhar, T. Lara, A., Sealy, P., Adesiyun, A. A. Microbial contamination of disinfectants and antiseptics in four major hospitals in Trinidad. Rev Panam Salud Publica/Pan Am J Public Health, 2003; 14 (3): 20.
Fraise, A. P. Choosing disinfectants. J. Hosp. Infect., 1999; 43 (4): 255-64.
Prasanthi, K., Murty, D. S., Nirmal, K. S. Evaluation of Antimicrobial Activity of Surface Disinfectants by Quantitative Suspension Method. International Journal of Research in Biological Sciences, 2012; 2 (3): 124-127.
Maurer, I. M. Hospital Hygiene; 3rd Edition, Richard Clay (The Chaucer Pren) Ltd., Great Britain, United Kingdom. 1985.
Rajesh, B., Rattan, L. Infection and Disease. In: Essentials of Medical Microbiology. Peromila, Editor. Jaypee Brothers Medical Publishers Ltd, 2008; pp. 65-68.
Mokhtari, M., Zandi, H., Jasmi, Z. T., Sahl, A. F., Montazari, A. The evaluation of efficacy of common disinfectants on microorganisms isolated from different parts of Shahid Sadoughi Accidents burns hospital in Yazd in 2011. Toloo e Behdasht, 2015; 14 (3): 1-11.
Miles, A. A., Misra, S. S. Bacterial counts. Journal of Hygiene (London), 1938; 38: 732.
Ochei, J. O., Kolhatkar, A. A. Plate Culture Methods In: Ochei, J. O., Kolhatkar, A. A. (eds.). Medical Laboratory Science: Theory and Practice, Tata McGrew-Hill, New Delhi, India. 2007b; pp. 591-592.
Cheesbrough, M. “Biochemical tests to identify bacteria” In: Cheesbrough, M. (ed.). District Laboratory Practice in Topical Countries, Part 2. Cambridge University Press, Cape Town, South Africa. 2006b; pp. 63-70.
Merap, O., Melike, E., Ekremkili, C. Evaluation of bactericidal activity of certain Gluteraldehyde free disinfectants used in the disinfection of endoscopes and surgical devices by a quantitative suspension test. Hacettepe University Journal of the Faculty of Pharmacy, 2007; 27: 131-138.
USEPA. Standard Operating Procedure for Quantitative Suspension Test Method for Determining Tuberculocidal Efficacy of Disinfectants against Mycobacterium bovis (BCG). US Environmental Protection Agency, Office of Pesticide Programs, Microbiology Laboratory, Environmental Science Center, 2014; SOP Number: MB-16-02. pp. 1-12.
Kelsey, J. C., Maurer, I. M. An improved Kelsey-Sykes test for disinfectants. Pharmaceutical Microbiology Journal, 2001; 6: 607-609.
Shott, S. “Statistics for health professionals”. Saunders, W. B., Co. Philadelphia, 1990; pp. 313-336.
Oie, S., Kamiya, A. Microbial contamination of antiseptics and disinfectants. Am J Infect Control; 1996; 24: 389–395.
Zembrzuska, S. E. The danger of infections of the hospitalized patients with the microorganisms present in the preparations and in the hospital environment. Acta Pol Pharm; 1995; 52: 173–178.
Obi, C., Muhammad U. K., Manga S. B., Atata R. F., Hauwa T. Assessment of commonly used Hospital Disinfectants on Bacteria isolated from the Operating Theatre. Journal of Bioscience and Biotechnology Discovery, 2016; 1: 59-65.
Yi-Shan, S., Gwo-Hwa, W., Ya-Wen, C., Hsiang-Lin, K., Li-Pien, L., Chiu-Hsin L., Huei-Shin, M. Effectiveness of Bacterial Disinfectants on Surfaces of Mechanical Ventilator Systems. Respiratory care, 2012; 57 (2): 255.
Radcliffe, C. E., Potouridou, L., Qureshi, R., Habahbeh, N., Qualtrough, A., Worthington, H., Drucker, D. B. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms Actinomyces israelii, A. naeslundii, Candida albicans and Enterococcus faecalis. Int Endod J., 2003; 37 (7): 438-446.
Virto, R., Mañas, P., Álvarez, I., Condon, S., Raso, J. Membrane damage and microbial inactivation by chlorine in the absence and presence of a chlorine-demanding substrate. Appl. Environ. Microbiol., 2005; 71: 5022–5028.
Bipasa, C., Nishith, K. P., Prasanta, K. M., Sanjit, K. P. and Raja, R. “Action of Newer Disinfectants on Multidrug Resistant Bacteria”. Journal of Evolution of Medical and Dental Sciences; 2014; 3 (11): 2797-2813.
Wijesinghe, L. P., Weerasinghe, T. K. A Study on the Bactericidal Efficiency of Selected Chemical Disinfectants and Antiseptics. OUSL Journal, 2010; 6: 44-58.
Awodele, O., Emeka, P. M., Agbamucle, H. C. and Akintonwa, A. (2007). The antimicrobial activities of some commonly used disinfectants on Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans. African Journal of Biotechnology. 6: 987-990.
Rutala, W. A., Barbee, S. L., Aguiar, N. C., Sobsey, M. D., Weber, D. J. Antimicrobial activity of home disinfectants and natural products against potential human pathogens. Infection Control and Hospital Epidemiology, 2000; 21 (1): 33-39.
Brooks, G. F., Carroll, K. C., Butel, J. S., Morse, S. A., Mietzner, T. A. (2013). “Diseases caused by Enterobacteriaceae other than Salmonella and Shigella”. In: a Lange Medical Book: Jawetz, Melnick & Adelberg’s Medical Microbiology (26th ed.). The Mcgraw-Hill Companies, Inc. 2013a; pp. 333-235.
Cheesbrough, M. “Bacterial pathogens” In: Cheesbrough, M. (ed.). District Laboratory Practice in Topical Countries, Part 2. Cambridge University Press, Cape Town, South Africa. 2006c; pp. 157-205.
Kayser, F. H., Bienz, K. A., Eckert, J., Zinkernagel, R. M. Bacteria as Human Pathogens In: Kayser, Medical Microbiology. Kayser, F. H. (ed.). Updated translation of the 10th German edition (2001): Medizinische Mikrobiologie, published by Georg Thieme Verlag, Stuttgart, Germany. Thieme Stuttgart, New York, USA. 2005; pp. 229-300.
Abilo, T., Meseret, A. Gram negative rods In: Medical Bacteriology. Lecture notes. University of Gondar in collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education. 2006; pp. 173-179, 231-249.
Brooks, G. F., Carroll, K. C., Butel, J. S., Morse, S. A., Mietzner, T. A. (2013). “Spore-Forming Gram-Positive Bacilli: Bacillus”. In: a Lange Medical Book: Jawetz, Melnick & Adelberg’s Medical Microbiology (26th ed.). The Mcgraw-Hill Companies, Inc. 2013b; pp. 175-178.
RMS. Pathogenicity and infectivity under immunosuppression of B. subtilis. Bacillus subtilis strain QST 713, Rapporteur Member State (RMS): German, 2001; 1: 19.
Cowan, S. T., Steel, K. J., Shaw, C., Duguild, J. P. A Classification of the Klebsiella group. Journal of General Microbiology, 1960; 23: 601.
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