Spleen Histological Changes Following Monosodium Glutamate Ingestion in Adult Male Wistar Rat
[1]
Idehen I. Charles, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
[2]
Bankole J. Kayode, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
[3]
Airhomwanbor Kingsley, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
[4]
DIC-Ijiewere O. Ebenezer, Department of Chemical Pathology, Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
[5]
Eidangbe A. Peace, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
This study investigates the effect of ingestion of monosodium glutamate (MSG) on the spleen histology of adult male Wistar rats. The study involved 30 adult rats (140 to 180grams). They were divided into five groups of 6 rats each: A (control; placed on water and rat chow feed), while groups B to E served as the test groups and received chow feed plus solution containing 40mg/kg bwt, 80mg/kg bwt, 120mg/kg bwt and 160mg/kg bwt of MSG respectively daily for 28 days. Their weights were monitored throughout the period of the experiment and standard care for laboratory animal was practiced. At the end of the 28th day, all animals were sacrificed and the spleen harvested following standard laboratory procedures and fixed in 10% formal saline for histological processing. The results showed test groups A and B presented normal spleen histology with no alteration in architectural arrangement of cells. However, test group C and D presented mild fatty degeneration and test group E showed moderate tissue changes. The observations suggest that MSG at high dose may be toxic to the spleen and may induce spleen tissue damages in a dose dependent manner. Based on our findings, doses above 40mg/kg body weight presented adverse effect on the spleen.
[1]
Nwajei JC., Onuoha SC., Essien EB. (2015). Effects of oral administration of selected food seasonings consumed in Nigeria on some sex hormones of Wistar albino rats. IOSR J Biotechnol. Biochem.; 1(5): 15-21.
[2]
George KR., Shibija NG., Malini NA. (2013). Monosodium glutamate (MSG) induced developmental dysfunction in female albino rats (Rattus norvegicus). Bioscan.; 8(1): 73-6.
[3]
Eweka A., Om'iniabohs F. (2007). Histological studies of the effects of monosodium glutamate on the ovaries of adult wistar rats. Ann. Med. Health Sci. Res.; 1(1): 37-43.
[4]
Husarova V., Ostatnikova D. (2013). Monosodium glutamate toxic effects and their implications for human intake: a review. JMEDRes.; 2013: 1-12.
[5]
Sharma A., Prasongwattana V., Cha'on U., Selmi C., Hipkaeo W., Boonnate P., et al. (2013). Monosodium glutamate (MSG) consumption is associated with urolithiasis and urinary tract obstruction in rats. PLoS One; 8(9): e75546.
[6]
US Food and Drug Administration (USFDA) (1995) FDA and Monosodium Glutamate (MSG) 1995 August 31. Bethesda, MD: U. S. Department of Health and Human Services.
[7]
Boonnate P., Waraasawapati S., Hipkaeo W., Pethlert S., Sharma A., Selmi C., et al. (2015). Monosodium glutamate dietary consumption decreases pancreatic b-cell mass in adult Wistar rats. PLoS One; 10(6): e0131595.
[8]
Maluly HDB., Areas MA., Borelli P., Reyes FGR. (2013). Evaluation of biochemical, hematological and histological parameters in non diabetic and diabetic Wistar rats fed with monosodium glutamate. Food Nutr. Sci.; 4(1): 66-76.
[9]
Kolawole OT. (2013). Assessment of the effects of monosodium glutamate on some biochemical and hematological parameters in adult Wistar rats. Am. J. BioSci.; 1(1): 11-5.
[10]
Walker R., Lupien JR. (2000). The safety evaluation of monosodium glutamate. J. Nutr.; 130(4S Suppl): 1049S-52S.
[11]
Pavlovic V., Sarac M. (2010). The role of ascorbic acid and monosodium glutamate in thymocyte apoptosis. Bratisl Lek Listy; 111: 357-360.
[12]
Williams AN., Woessner KM. (2009). Monosodium glutamate ‘allergy’: menace or myth? Clin. Exp. Allergy; 39: 640-646.
[13]
Kulkarni AD., Sundaresan A., Rashid MJ., Yamamoto S., Karkow F. (2014). Application of Diet-derived Taste Active Components for Clinical Nutrition: Perspectives from Ancient AyurvedicMedical Science, Space Medicine, and Modern Clinical Nutrition. Current pharmaceutical design 20: 2791-2796.
[14]
Von Diemen V., Trindade EN., Trindade MR. (2006). Experimental model to induce obesity in rats. ActaCir. Bras.; 21: 425-429.
[15]
Mi_skowiak B., Partyka M. (1993). Effects of neonatal treatment with MSG (monosodium glutamate) on hypothalamo-pituitary-thyroid axis in adult male rats. Histol Histopathol.; 8(4): 731-4.
[16]
Amresh G., Singh PN., Rao CV. (2008). Toxicological screening of traditional medicine Laghupatha (Cissampelos pareira) in experimental animals. J. Ethnopharmacol.; 116(3): 454-60.
[17]
Mebius RE., Kraal G. (2005). Structure and function of the spleen. Nat Rev Immunol.; 5: 606-616.
[18]
Onkar DP., Govardhan SA. (2013). Comparative histology of human and dog spleen. J Morphol Sci.; 30:16-20.
[19]
Elmore SA. (2006). Enhanced histopathology of the spleen. Toxicol. Pathol.; 34: 648-655.
[20]
Hassan ZA., Arafa MH., Soliman WI., Atteia HH., Al-Saeed HF. (2014). The effects of monosodium glutamate on thymic and splenic immune functions and role of recovery (biochemical and histological study). J. Cytol. Histol.; 5: 6.
[21]
Savcheniuk OA., Virchenko OV., Falalyeyeva TM., Beregova TV., Babenko LP., Lazarenko LM., et al. (2014). The efficacy of probiotics for monosodium glutamate-induced obesity: dietology concerns and opportunities for prevention. EPMA J.; 5(1): 2.
[22]
Collison KS., Zaidi MZ., Saleh SM., Inglis A., Mondreal R., Makhoul NJ., et al. (2011). Effect of trans-fat, fructose and monosodium glutamate feeding on feline weight gain, adiposity, insulin sensitivity, adipokine and lipid profile. Br. J. Nutr.; 106(2): 218-26.
[23]
Hermanussen M., García AP., Sunder M., Voigt M., Salazar V., Tresguerres JA. (2006). Obesity, voracity, and short stature: the impact of glutamate on the regulation of appetite. Eur. J. Clin. Nutr.;; 60(1): 25-31.
[24]
Gobatto CA., Mello MA., Souza CT., Ribeiro IA. (2002). The monosodium glutamate (MSG) obese rat as a model for the study of exercise in obesity. Res. Commun. Mol. Pathol. Pharmacol.; 111(1–4): 89-101.
[25]
Kondoh T., Torii K. (2008). MSG intake suppresses weight gain, fat deposition, and plasma leptin levels in male Sprague-Dawley rats. Sep 3;95(1-2):135-144.
[26]
Ciric M., Cekic S., Pavlovic V., Jovic Z., Tasic G. (2005) Histopathological changes In spleen of rats treated with monosodium glutamate. Acta Fac. Med. Naiss; 22:191-194.
[27]
Onyema OO., Farombi EO., Emerole GO., Ukoha AI., Onyeze GO. (2006). Effect of Vitamin E on Monosodium Glutamate Induced Hepatotoxicity and Oxidative Stress in Rats, Indian J. Biochem. Biophysics; 43(1), 20-4.
[28]
Ebaid HM., Tag HM. (2012). Monosodium glutamate toxic effect on spleen structure and potential of recovery in adult albino rats. Egypt Acad. J. Biol. Sci.; 4: 1-8.
[29]
Samuels A. (1994). Excitatory Amino Acids in Neurologic Disorders. New England J. Med.; 331(4): 274-5, July 28, 1994.
[30]
Olson H., Betton G., Stritar J., Robinson D. (1998). The predictivity of the toxicity of pharmaceuticals in humans from animal data—An interim assessment. Toxicol. Lett.; 102–103, 535–538.
