Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News
Morphological and Molecular Tools for Identification of Saccharomyces cerevisiae Isolated from Chinese Product
Current Issue
Volume 5, 2018
Issue 2 (June)
Pages: 24-28   |   Vol. 5, No. 2, June 2018   |   Follow on         
Paper in PDF Downloads: 50   Since May 29, 2018 Views: 1181   Since May 29, 2018
Authors
[1]
Ahmed Hassan Mousa, Department of Food and Dairy Science and Technology, Faculty of Environmental Agricultural Science, Arish University, El Arish, Egypt; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China.
[2]
Gang Wang, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China; International Joint Research Center for Probiotics & Gut Health, School of Food Science and Technology, Jiangnan University, Wuxi, China; Institute of Food Biotechnology (Yangzhou), Jiangnan University, Yangzhou, China.
[3]
Hao Zhang, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China; International Joint Research Center for Probiotics & Gut Health, School of Food Science and Technology, Jiangnan University, Wuxi, China; National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, Wuxi, China; Institute of Food Biotechnology (Yangzhou), Jiangnan University, Yangzhou, China.
Abstract
Isolation and identification of Saccharomyces cerevisiae from a Chinese product "High Active Dry Yeast" were presented in this study. The morphological identification approached the isolated yeast to S. cerevisiae that was revealed by the circular shapes, elliptical morphology, and pseudohyphae of the colonies. Identification of yeast genetically processed by using the primer pair NL1 and NL4 to amplify and sequence the 26S rDNA gene D1/D2 domain. The internal transcribed spacer was amplified and sequenced by using ITS1 and ITS4 for fungus identification, besides using the primer pair of NS1 and NS6. Discrimination of yeast from bacteria processed by using the primer pair 7F, 1540R, and another primer pair 27F, and 1492R. PCR technique could approve that the S. cerevisiae was the only frequent strain in the Chines product. The photographed pattern by UV transilluminator types the target DNA band to S. cerevisiae that was achieved by amplifying and sequencing the 26S rDNA regions.
Keywords
Saccharomyces cerevisiae, PCR Technique, 26S rDNA Gene, Morphological Characteristics
Reference
[1]
H. Feldmann, Yeast: molecular and cell biology, John Wiley and Sons, 2011.
[2]
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M. Life with 6000 genes. Science 1996; 274: 546–67.
[3]
Botstein D, Chervitz SA, Cherry M. Yeast as a model organism. Science 1997; 277; 1259–60.
[4]
W. Li, Molecular evolution, Sinauer associates incorporated, 1997.
[5]
Cai J, Roberts IN, COLLINS MD. Phylogenetic relationships among members of the ascomycetous yeast genera Brettanomyces, Debaryomyces, Dekkera, and Kluyveromyces deduced by small-subunit rRNA gene sequences. International Journal of Systematic and Evolutionary Microbiology 1996; 46: 542–49.
[6]
Duongthingoc D, George P, Gorczyca E, Kasapis S. Studies on the viability of Saccharomyces boulardii within microcapsules in relation to the thermomechanical properties of whey protein. Food Hydrocolloids 2014; 42: 232–38. doi: 10.1016/j.foodhyd.2013.07.024.
[7]
Vanhee LME, Goemé F, Nelis HJ, Coenye T. Quality control of fifteen probiotic products containing Saccharomyces boulardii. Journal of Applied Microbiology 2010; 109: 1745–52. doi: 10.1111/j.1365-2672.2010.04805.x.
[8]
D. H. Larone, Yeast and yeast-like organisms, Medically Important Fungi: A Guide to Identification, 5th ed., D. H. Larone, Washington: ASM Press-DC, 2011.
[9]
Mu Z, Yang X, Yuan H. Detection and identification of wild yeast in Koumiss. Food Microbiology 2012; 31: 301–8. doi: https://doi.org/10.1016/j.fm.2012.04.004.
[10]
Fietto JLR, Araújo RS, Valadão FN, Fietto LG, Brandão RL, Neves MJ, Gomes FCO, Nicoli JR, Castro IM. Molecular and physiological comparisons between Saccharomyces cerevisiae and Saccharomyces boulardii. Canadian Journal of Microbiology 2004; 50: 615–21.
[11]
Pataro C, Guerra JB, Petrillo-Peixoto ML, Mendonça-Hagler LC, Linardi VR, Rosa CA. Yeast communities and genetic polymorphism of Saccharomyces cerevisiae strains associated with artisanal fermentation in Brazil. Journal of Applied Microbiology 2000; 89: 24–31.
[12]
Couto MMB, Eijsma B, Hofstra H, van der Vossen JM. Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains. Applied and Environmental Microbiology 1996; 62: 41–46.
[13]
James SA, Collins MD, Roberts IN. Genetic interrelationship among species of the genus Zygosaccharomyces as revealed by small-subunit rRNA gene sequences. Yeast 1994; 10: 871–81.
[14]
Waghmare SK, Bruschi CV. Differential chromosome control of ploidy in the yeast Saccharomyces cerevisiae. Yeast 2005; 22: 625–39.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
CONTACT US
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
E-mail:
LET'S GET IN TOUCH
Name
E-mail
Subject
Message
SEND MASSAGE
Copyright © 2013-, Open Science Publishers - All Rights Reserved