Phytochemical Screening, GC - MS Analysis, Antibacterial and Antioxidant Activity of Seeds Oil of Annona Squmosa L. Sudanese Medicinal Plant
Tuhami Elzein Hagr, Department of Chemistry and Industrial Chemistry, College of Applied and Industrial Sciences, University of Bahri, Khartoum, Sudan.
Ibrahim Abdurrahman Adam, Department of Basic Sciences, University of Zalingei, Zalingei, Sudan.
Abdelrahman Ahmed Almain, Department of Chemistry and Industrial Chemistry, College of Applied and Industrial Sciences, University of Bahri, Khartoum, Sudan.
Manhal Musa Mohammed, Department of Chemistry and Industrial Chemistry, College of Applied and Industrial Sciences, University of Bahri, Khartoum, Sudan.
The objective of this the study was examine Phytochemical analysis of A. squmosa ethanolic extracts and investigating the chemical constituent’s and to assess it potential antibacterial and antioxidant activities of Sudanese A. squmosa seeds oil. Using cold maceration and Soxhlet method to extract the A. squmosa seeds, where DPPH assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that Alkaloids, Flavonoid, Carbohydrate, Saponins, Triterpene, Streol, Tannins and phenolic compounds were present in the A. squmosa seeds ethanolic extracts where GC-MS analysis detected Twenty-one components in the A. squmosa seeds oil. Five of them are major namely, Hexadecanoic acid (18.48), Heptadecanoic acid Methyl margarate (1.32%), 9,12-octadecadienoic acid (Z, Z) (19.29% ), 9 - octadecenoic acid (Z) (34.01%), Methyl stearate (17.12% ), Cis-11-Eicosenoic acid (1.14% ) and Eicosanoic acid (3.28%.). The DPPH assay, showed moderate antioxidant potential (50 ± 0.09 compared with standard 93± 0.01; the antibacterial showed showed high inhibitory effect against Pseudomonas aeruginosa (16mm), Bacillus subtilis (15mm), Candida albicans (15mm) moderate against Escherichia coli (13mm) and Candida albicans.
Phytochemical Screening, GC-MS Analysis, Antibacterial and Antioxidant Activities
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