Ameliorative Effects of Combined Administration of Lycopene and/or Zinc on Biomarkers of Oxidative Stress in Alloxan- Induced Diabetic Wistar Rat
[1]
Goji Anthony Donatus Teru, Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medicine Kaduna State University, Kaduna, Nigeria.
[2]
Muhammed Kabir Ahmed, Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medicine Kaduna State University, Kaduna, Nigeria.
[3]
Isah Anthony Denis, Department of Obstetrics and Gynaecology, University of Abuja Teaching Hospital, Abuja, Nigeria.
[4]
Medina Sani, Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, Ahmadu Bello University, Zaria, Nigeria.
Diabetes mellitus remains a burdensome and public health problem with increasing global. The current global prevalence of diabetes mellitus, 285 million people in 2010, is estimated to be 439. This work was designed to study the effects of combine administration of lycopene and zinc on some biomarkers of oxidative stress in alloxan- induced diabetic rats. Apparently healthy albino rats weighing between 150g and 200g were used. The rats were randomly allotted into six groups, each containing five albino rats respectively. Five of the groups (II, III IV V and VI) were induced with diabetes by single intraperitoneal (i.p) injection of freshly prepared in 0.1 mol/L citrate buffered solution (pH 4.5) of alloxan (Sigma Aldrich, St. Louis, MO, USA) at a dose of 150 mg/kg body weight. Control (vehicle) rats were injected with equal volume of 0.1 mol/L citrate buffer. Four days after alloxan injection, diabetes induction was confirmed by measuring fasting blood glucose level in a tail vein blood samples using ACCU-CHEK compact plus glucometer (Roche, France). Rats with glucose level of 200 mg/dl or higher were considered as diabetic. After the induction of diabetes the rats were treated using the Lycopene and zinc separately and in combination respectively according to group daily, whereas, the other group (I) was not given any treatment and this served as the normal control, providing a baseline data. The results indicated that oral supplementation of lycopene (10 mg/kg b.w /day) and zinc (20mg/kg b.w /day) separately or in combination for 4 weeks of treatment exhibited significant alterations in the alloxan-induced-type-1 diabetes. The result showed that there was an increase in the serum CAT activity, SOD and GPx levels of the group treated with lycopene supplement as compared to the diabetic control group. The result also showed a significant p<0.05 increase in CAT, SOD and GPx levels in the group treated with zinc supplement when compared with the diabetic control group. There was also a significant p<0.05 increase in SOD and GPx levels in the group treated with zinc and lycopene as compared with the diabetic control. There was also a decrease in MDA concentrations for the groups treated with lycopene, zinc and the combined regimen as compared to the diabetic control. In conclusion, our finding indicates better ameliorative effects of combined treatment with lycopene and zinc for the oxidative stress in alloxan-induced diabetic rats.
Diabetes, Alloxan, Lycopene, Zinc, Wistar Rats
[1]
Paul, c., Okey, O and Angomuo, O. (2015). Oxiadtive Stress in Diabetes Mellitus. International Journal of Biological Chemistry, 9 (3): 92-109.
[2]
Uhuo, E. M., Ogugua, V., Joshua, N and Paker, E. (2015). Effects of Solvent Extracts of Colatropis gigantean leaf on Oxiadtive Parameters in Diabetic Rabbits. International Journal of Medicine and Medical Sciences, 7 (6): 108-116.
[3]
Shatadal, G., Sudip, B., Kahkashan, R and Parames, C. (2015). Curcumin Protects Rats Liver from STZ-induced diabetic Pathophysiology by Counteracting Reactive Oxygen Species and inhibiting the activation of P53 and MAPKs mediated Stress response Pathways, Vol 2 Pages 365-376.
[4]
Nagy, M. A. (2015). Biochemical Effects of Cleome droserifolia on Alloxan-Induced Diabetes in Rats: Role of Insulin, Oxidative Stress and Inflammation, Biochemistry an Indian Jourmal, 9 (2): 61-67.
[5]
Aaetha, C., Rajeev, C and Shalini, J. (2016). Microvascular and Macrovascular complications in Diabetes Mellitus: Distinct or Continnum. Indian Journal of Endocrinology and metabolism, 20 (4): 546-551.
[6]
Chan, D. C. and Watts, GF. (2011). Dyslipidaemia in the metabolic syndrome and type 2 diabetes: pathogenesis, priorities, pharmacotherapies. Expert Opin Pharmacother 12 (1): 13-30.
[7]
Piero, M. N., Nzaro, G. M and Njagi, J. M. (2015). Diabetes Mellitus- a Devastating Metabolic Disoder. Asian Journal of Biomedical and Pharmaceutical Sciences, 4 (40): 1-7.
[8]
Hfaiedh, N., Murat, J. C. and Elfeki, A. (2013). Diabetes-induced damages in rat kidney and brain protective effects of natural antioxidants. Journal of Nutrition and Food Science, 3: 4-6.
[9]
Martin, J. P., Dailey, M. and Sugarman, E. (1987). Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Archives of Biochemistry and Biophysics, 255: 329-336.
[10]
Beer, R. F. and Sizer, I. W. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195: 133-140.
[11]
Takebe, G. (2002). A comparative study on the hydroperoxide and thiol specificity of the glutathione peroxidase family and selenoprotein. Journal of Biological Chemistry, 277 (43): 41254-41258.
[12]
Janero, D. R. (1990). Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free-Radical Biology and Medicine, 9: 515-540.
[13]
Duncan, R. C., Knapp. R. G. and Miller, M. C. (1977). “Test of Hypotheses in Population Means” In Introductory Biostatistics for Health Science, N. Y.: John Wiley and SonInc, 71-6.
[14]
Singh, K., Bal, B. S., Chopra, S., Singh, S., and Malhotra, N. (2012). Ameliorative Effect Lycopene on Lipid Peroxidation and certain antioxidant enzymes in diabetic patients. Journal of Diabetus, 6 (5): 3-4.
[15]
Ranasinghe, P., Pigera, S., Galappatthy, P., Katulanda, P. and Constantine, G. R. (2015). Zinc and diabetes mellitus: understanding molecular mechanisms and clinical implications. Daru Journal of Pharmaceutical Sciences, 23 (1): 44.
[16]
Eze, D. E., Yusuf, T., Abubakar, A., Suleiman, O. S., Rabiu., M. K. and Mohammed, A. (2017). Lycopene Ameliorates Diabetic-Induced Changes in Erytrocytes Osmotic Fragility and lipid peroxidation in Wistar Rats. Journal of Diabetes Mellitus 7 (3): 71-85.
[17]
Stahl, W., Heinrich, U., Aust, O., Tronnier, H. and Sies, H. (2006). Lycopene-rich products and dietary photoprotection. Photochemical and Phototherapy Research, 15 (2): 238-242.
[18]
Chen, J., Yang,-Song, Y., and Zhang, L. (2013). Effect of Lycopene supplementation on oxidative Stress: An Exploratory Systematic Review and Meta- Analysis of Randomised Controlled Trials. Journal of Medicinal Food, 16 (5): 361-374.
[19]
Cruz, K. J. C., Oliveira, A. R. S., and Marreiro, D. N (2015). Antioxidant role of zinc in diabetes mellitus. World Journal of Diabetes 6 (2): 333-337.
