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Determination of Plasmodium Specific Immunoglobulin G and M in Patients with Malaria in Ikorodu, Lagos, Nigeria
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Volume 6, 2018
Issue 1 (February)
Pages: 1-7   |   Vol. 6, No. 1, February 2018   |   Follow on         
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Elekhebor E. John, Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, University of Benin, Benin City, Nigeria.
Wellington A. Oyibo, Department of Medical Microbiology and Parasitology, College of Medicine University of Lagos, Lagos, Nigeria.
Dic-Ijiewere O. Ebenezer, Department of Chemical Pathology, Faculty of Clinical Sciences, College of Medical Sciences, Ambrose Alli University, Ekpoma, Nigeria.
Airhomwanbor O. Kingsley, Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Nigeria.
Idehen I. Charles, Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Nigeria.
This study was conducted to determine the presence of Plasmodium specific IgG/IgM, its relationship with clinical symptoms, parasite density, age and sex in four primary health care facilities in Ikorodu Lagos, Nigeria. A total number of 1218 patients 450(36.9%) male and 768(63.1%) female were recruited for the study. Microscopy method was used to screen the patients of which 235 were positive for malaria parasite. A total of 167 sera was pooled out from the positive samples and tested for Plasmodium specific IgG/IgM using enzyme linked immunosorbent assay (ELISA). 135(83.9%) were positive for Plasmodium specific IgG/IgM and presenting with fever, while 26(16.1%) were negative with fever. The parasite density was 30671.71 ± 31809.3 with majority of participants that had moderate parasitaemia and presented with fever, having higher positivity for Plasmodium specific IgG/IgM 41(89.15%) while 5(10.9%) with fever and moderate parasitaemia were negative for Plasmodium specific IgG/IgM. There was no significant correlation between IgG/IgM and clinical signs/symptom, parasite density, age and sex (P > 0.05). These findings demonstrated that individuals living in this community mount a strong antibody response against P. falciparum parasites and these antibodies persist as a result of infection and re-infection irrespective of clinical presentation, age and sex differences.
Malaria, Plasmodium, Immunoglobulin, Primary Health
World Health Organization (2013). World Malaria Report. Geneva, Switzerland.
Clark, T. D., Greenhouse, B., NjamaMeya, D., Nzarubara, B., Maiteki-Sebuguzi, C., Staedke, S. G., Seto, E., Kamya, M. R., Rosenthal, P. J., Dorsey, G: (2008) Factors determining the heterogeneity of malaria incidence in children in Kampala, Uganda. J InfectDis198:393-400.
Kreuels, B., Kreuzberg, C., Kobbe, R., Ayim-Akonor, M., Apiah-Thompson, P., Thompson, B., Ehmen, C., Adjei, S., Langefeld, I., Adjei, O., May, J: (2010) Differing effects of HbS and HbC traits on uncomplicated falciparum malaria, anemia, and child growth. Blood 115:4551-4558.
Gong, L., Maiteki-Sebuguzi, C., Rosenthal, P. J., Hubbard, A. E., Drakeley, C. J., Dorsey, G., Greenhouse, B: (2012) Evidence for both innate and acquired mechanisms of protection from Plasmodium falciparum in children with sickle cell trait. Blood 119:3808-3814.
Perlmann, P., Troye-Bloomberg, M., (2002). Malaria and the Immune system in Humans. Malaria Immunology. Chemical Immunology. Basel, Karger 80:229-242.
Beeson, J. G., Osier, F. H. A. (2008): Engwerda C R. Recent insights into humoral and cellular immune responses against malaria. Trends Parasitol. 24(12):578–584.
Doolan, D. L., Dobaño, C., Baird, J. K. (2009): Acquired immunity to malaria. Clin Microbiol Rev. 22(1):13–36.
Stanisie, D. I., Richards, J. S., McCallum, F. J. (2009) “Immunoglobulin G subclass-specific responses against Plasmodium falciparum merozoite antigens are associated with control of parasitaemia and protection from symptomatic illness,” Infection and Immunity, vol. 77, no. 3, pp. 1165–1174.
Tangteerawatana, P., Krudsood, S., Chalermrut, K., Looareesuwan, S., Khusmith, S. (2001): Natural human IgG subclass antibodies to Plasmodium falciparum blood stage antigens and their relation to malaria resistance in an endemic area of Thailand Southeast. South-East Asian J Trop Med Public 32:247-254.
Garraud, O., Mahanty, S., Perraut, R. 2003. Malaria-specific antibody subclasses in immune individuals: a key source of information for vaccine design. Trends Immunol 24:30.
Aucan, C., Traore, Y., Tall, F., Nacro, B., Traore-Leroux, T., Fumoux, F, Rihet, P. (2000). High Immunoglobulin G2 (IgG2) and low IgG 4 Levels are Associated with Human Resistance to Plasmodium falciparum Malaria. Infect Immun 68:1252-1258.
Joana, D., Fabian, H., Vincent, G., Jean-Francois, F., Jacques, R., Pierre-Andre, C., Dominique, M., Maryvonne, K., Constantin, F., Sylviane, P. (2012). High levels of IgE auto antibody to 14-3-3epsilon protein correlate with protection against severe Plasmodium falciparum malaria, the journal of infectious disease
Desowitz, R. S., Elm, J., Alpers, M. P (1993): Plasmodium falciparum–Specific immunoglobulin G (IgG), IgM and IgE antibodies inpaired maternal-cord sera from East Sepik Province, Papua New Guinea. Infect Immun 61:988-993.
Maeno, Y., Steketee, R., Nagatake, T., Tegoshi, T., Desowitz, R. S., Wirima, J. J., Aikawa, M. (1993): Immunoglobulin complex deposits in Plasmodium falciparum-infected placentas from Malawi and Papua New Guinea. Am J Trop Med Hyg 1993, 49:574-580.
Maeno, Y., Perlmann, P., Perlmann, H., Kusuhara, Y., Taniguchi, K., Nakabayashi, T., Win, K., Looarreesuwan, S., Aikawa, M. (2000): IgE Deposition in brain microvessels and on parasite erythrocytes from cerebral malaria patients. Am J Trop Med Hyg, 63: 128-132. Matters most, quantity or quality? Mem Inst Oswaldo Cruz. 87 Suppl3:229-234.
Desowitz, R. S., (1989). Plasmodium Specific Immunoglobulin E in Sera from an Area of Holo endemic Malaria. Trans R Soc Trop Med Hyg, 83:478-479.
Perlmann, H., Helmby, H., Hagstedt, M., Carlson, J., Larsson, P. H., Troye-Blomberg, M., Perlmann, P. (1994): IgE elevation and IgE anti-malarial antibodies in Plasmodium falciparum malaria: Association of high IgE levels with cerebral malaria. Clin Exp Immunol 97:284-292.
Duarte, J., Deshpande, P., Guiyedi, V., Mécheri, S., Fesel, C., Cazenave, P-A., Mishra, G. C., Kombila, M., Pied, S. (2007): Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status. Malar J 6:1-13.
Jayawardena, A. N., Janeway, C. A., Kemp, J. D. (1979): Experimental malaria in the CBA/Nmouse. J Immunol 123:2532-2539.
Branch, O. H., Udhayakumar, V., Hightower, A. W., Oloo, A. J., Hawley, W. A., Nahlen, B. L. Bloland, P. B., Kaslow, D. C., Lal, A. A., (1998). A Longitudinal Investigation of IgG and IgM Antibody Response to Merozoite specific Protein-119kDa Domain of Plasmodium falciparum in Pregnant Women and Infants: association with Febrile Illness, Parasitemia and Anemia. Am J Trop Med Hyg 58:211-219.
National Population Commission (NPC) (2006). Population and Housing Census, Population distribution by Sex, State, LGA, and Senatorial district, http://www.population.gov.ng.
Perignon, J. L., and P. Druilhe. (1994). Immune mechanisms underlying the premonition against Plasmodium falciparum malaria. Mem.Inst.Oswaldo Cruz 89(Suppl.2):51-53
Shi, Y. P., Sayed, U., Qari, S. H., Roberts, J. M., Udhayakumar, V., Oloo, A. J., Hawley, W. A., Kaslow, D. C., Nahlen, B. L., Lal, A. (1996). Natural immune response to the C-terminal 19-kilo Dalton domain of Plasmodium falciparum merozoite surface protein1. InfectImmun64:2716.
Egan, A. F., Morris, J., Barnish, H., Allen, S., Greenwood, B. M., Kaslow, D. C., Holder, A. A., Riley, E. M. (1996). Clinical immunity to Plasmodium falciparum malaria is associated with serum antibodies to the 19-kDaC-terminal fragment of the merozoite surface antigen, PfMSP-1. J Infect Dis 173:765.
Dodoo, D., T. G. Theander, J. A. L. Kurtzhals, et al. 1999. Levels of antibody to conserved parts of Plasmodium falciparum merozoite surface protein 1 in Ghanaian children are not associated with clinical protection from malaria. Infect. Immun.67:2131-2137
Soe, S., Theisen, M., Roussilhon, C., Aye, K. C., Druilhe, P. (2004). Association between protection against clinical malaria and antibodies to merozoite surface antigens in an area of hyperendemicity in Myanmar: complementarity between responses to merozoite surface protein 3 and the 220-kilodalton glutamate-rich protein. Infect Immun 72:247.
Perlmann, P., Troye Blomberg, M. (2002). Malaria and the Immune System in Humans. Malaria Immunology (2nded.), vol80. (pp. 229-242).
Aribot, G. C., Rogier, J. L., Sarthou, J. F., Trape, A. T., Balde, P. D., Roussilhon, C. (1996). Pattern of immunoglobulin isotype response to Plasmodium falciparum blood-stage antigens in individuals living in a holoendemic area of Senegal (Dielmo, west Africa). Am J Trop Med Hyg 54:449.
Taylor, R. R., Allen, S. J., Greenwood, B. M., Riley, E. M. (1998). IgG 3 antibodies to Plasmodium falciparum merozoite surface protein 2 (MSP2): increasing prevalence with age and association with clinical immunity to malaria. Am J Trop Med Hyg 58: 406.
Cavanagh, D. R., Elhassan, I. M., Roper, C., Robinson, V. J., Giha, H., Holder, A. A., HviidL., Theander, T G, Arnot, D. E., McBridge J. S., (1998). A Longitudinal study of Type-Specific Antibody Responses to Plasmodium falciparium Merozoite Surface Protein-1 in an Area of Unstable Malaria in Sudan. J Immunol 161:347-359.
Boutlis, C. S., Fagan, P. K., Gowda, D. C., Lagog, M., Mgone, C. S., Bockarie, M. J., Anstey, N. M. (2003). Immunoglobulin G (IgG) Responses to Plasmodium falciparum Glycosyl phosphatidyl inositols Are Short-Lived and Predominantly of the IgG3 Subclass J Infect Dis. 187(5):862-865.
Kinyanjui, S. M., Conway, D. J., Lanar, D. E., Marsh, K., (2007). IgG Antibody Responses to Plasmodium falciparum Merozoite Antigens in Kenyan Children have a Short Half-Life. Malaria Journal 6:82-89.
McCallum F. J., Persson, K. E., Sutherland, C. J., Mugyenyi, C. K., Fowkes, F. J., (2008) The Dynamics of Naturally Acquired Immunity to Plasmodium falciparum Infection PLoS Comput Biol›v.8(10).
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