Liquid Chromatographic and Potentiometric Methods for the Determination of Oxomemazine in Pharmaceutical Preparations
[1]
Mohamed Alaa Fathy Elmosallamy, Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt.
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Amr Lotfy Saber, Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt; Department of Chemistry, Faculty of Science, Umm Al Qura University, Makkah, Saudi Arabia.
[3]
Alaa Said Amin, Department of Chemistry, Faculty of Science, Benha University, Benha, Egypt.
[4]
Hamada Mohammed Ahmed Killa, Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt.
Simple and selective two different techniques are developed for the determination of oxomemazine in pharmaceutical preparations. A rapid (less than 6 min) and accurate high performance liquid chromatography(HPLC) method has been developed where chromatographic analysis is performed on Nova-Pak® C18 column (3.9 mm × 150 mm, 5 µm) with an ammonium formate buffer adjusted with formic acid to pH 3.0 and acetonitrile (75: 25, v/v) as a mobile phase, and detection at 235 nm. Good linearity (0.99, r 2), accuracy (≥ 99.20%), and precision (≤ 0.6 RSD) were obtained. Potentiometric measurements are based on tetrakis (p -chlorophenyl) borate-oxomemazine ion-pair complex as an electroactive species incorporating in a plasticized poly vinyl chloride (PVC) membrane with o-nitrophenyl octyl ether (o -NPOE) or dioctyl phthalate (DOP). The sensor exhibits fast and stable Nernstian response for oxomemazine over the concentration range of 1.0 × 10-5 – 1.0 × 10-2 mol L-1 and pH range of 3.5 - 6.0. The sensor shows reasonable selectivity towards oxomemazine hydrochloride over many cations. No significant interferences are caused by drug excipients and diluents. Results with an average recovery of 100.6% and a mean standard deviation of 0.77% of the nominal were obtained.
HPLC, Potentiometry, Oxomemazine Hydrochloride, Pharmaceutical Preparations
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