Evaluation of Some Traditional Antidiabetic Plants on Testis in the Alloxan-Induced Diabetic Male Albino Rats: Histological, Histochemical, Immunohistochemical and Morphometric Study
[1]
Tamer M. M. Abu-Amara, Histology and Cytology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
[2]
Abd EL Razek A. Meselhy, Anatomy and Embryology Department, Faculty of Medicine, Al-Azhar University, Assiut, Egypt.
[3]
Howada I. Abdel Aziz, Histology and Cytology Department, Faculty of Medicine, Suze-Canal University, Ismailia, Egypt.
[4]
Amal Said, Zoology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.
Background: Diabetes mellitus is one of the common and widely distributed metabolic diseases all over the world. This disease is characterized by hyperglycemia that results from defects in insulin secretion, insulin action or both. Previous studies reported negative complications of diabetes on the male genital system with associated gonadal dysfunction. Different medicinal plant species were used as a traditional treatment for diabetes mellitus long times ago. Aim of the work: As diabetes had been reported to affect the male genital system, this work was aimed to investigate the antidiabetic effects of the aqueous extract of the following herbs: Ambrosia maritima, L. (Damsissa), Chrozophoratinctoria, L. (Sammo), Artemisia annua, L. (Kaysom) and Artemisia judaica, L. (Sheeh) on the testis’ histology in the alloxan-induced diabetic male albino rats. Material and Methods: This study was performed on sixty male albino rats with an average 100-110 g body weight. The animals were divided into six groups (10/cage); Group1 (Control untreated-group), Group 2 (Alloxan-induced diabetic group), Group 3 (diabetic group treated orally with “28.5 mg/kg body wt. twice/day” of the Damsissa extract), Group 4 (diabetic group treated orally with “28.5 mg/kg body wt. twice/day” of the Sammo extract), Group 5(diabetic group treated orally with “28.5 mg/kg body wt. twice/day” of the Kaysom extract)and Group 6 (diabetic group treated orally with “28.5 mg/kg body wt. twice/day” of the Sheeh extract). Results: Considerable improvements in the testicular tissue morphological changes that were observed in diabetic groups had been detected after treatment with Damsissa and Sammo in comparison to the control group. These improvements were less obvious after Kaysom and Sheeh treatment. Conclusion: It could be concluded that Damsissa and Sammo can guard against the negative effects of diabetes on the testis.
Diabetes Mellitus, Alloxan, Hyperglycemia, Damsissa, Sammo, Kaysom, Sheeh
[1]
Yanardag, R., Ozsoy-Sacan O., Bolkent, S. (2005): Protective effects of metform in treatment on the liver injury of streptozotocin-diabetic rats. Hum ExpToxicol 2005, 24: 129-135.
[2]
Cai L., Chen S., Evans, T. (2000): Apoptotic germ-cell death and testicular damage in experimental diabetes: prevention by endothelin antagonism. Urol Res., 28: 342-347.
[3]
Orth, M., Murray, T., Bardin, W. (1979): Ultrastructural Changes in Leydig Cells of Streptozotocin-induced Diabetic Rats. Anat Rec., 195: 415-430.
[4]
Kuhn-Velten, N., Waldenburger D., Staib, W. (1982): Evaluation of Steroid Biosynthetic Lesions in Isolated Leydig Cells from the Testes of Streptozotocin-Diabetic Rats. Diabetol, 23: 529-533.
[5]
Morimoto, S., Mendoza-Rodriguez, A., Hiriat, M. (2005): Protective effect of testosterone on early apoptotic damage induced by streptozotocin in rat pancreas. J Endocrinol, 187: 217-224.
[6]
Ballester, J., Carmen, M., Dominguez, J. (2004): Insulin-Dependent Diabetes Affect Testicular Function by FSH- and LH-linked Mechanisms. J Androl, 25: 706-719.
[7]
Cameron, F., Orth, J., Murray T. (1982): Morphological alteration in the testes from diabetic man and rat. Diabetes., 31: 11A.
[8]
Steger, W. and Rabe, M. (1997): The effect of diabetes mellitus on endocrine and reproductive function. ProcSocExpBiol Med., 214: 1-11.
[9]
Ozdemir, O., Akalin, P., Baspinar, N. (2009): Pathological changes in the acute phase of streptozotocin-induced diabetic rats. Bull Vet InstPulawy, 53: 783-790.
[10]
Sanguinetti, E., Ogawa K., Kurohmaru, M. (1995): Ultrastructural changes in mouse Leydigcells after streptozotocin administration. Exp Anim., 44: 71-73.
[11]
Ozturk, F., Gul, M., Agkadir, M. (2002): Histological alterations of rat testes in experimental diabetes. T Kin J Med Sci., 22: 173-178.
[12]
Navarro-Cassado, L., Juncos-Tobarra, MA., Chafer-Rudilla, M. (2010): Effect of experimental diabetes and STZ on male fertility capacity. Study in rats. J Androl., 108: 007260.
[13]
Abdel Wahab, M., Wassel, M., Ammar, M. and Hanna, T. (1987): HerbaHungarica Tom., 26 (1): 27-31.
[14]
Ghazanfer, S. (1994): CRC Hand book of Arabian Medicinal Plants. CRC Press, Boca Raton., Pp. 265.
[15]
Bora, KS., and Sharma, A. (2011): The genus Artemisia: A comprehensive review. Pharm. Biol., 49: 101–109.
[16]
Al-Mustafa, H., and Al-Thunibat, OY. (2008): Antioxidant capacity of some Jordanian medicinal plants used traditionally for the treatment of diabetes. Pak J Biol Sci., 1: 351–358. http://www.ncbi.nlm.nih.gov/pubmed/1881715
[17]
Bhakuni, S., Jain, C., Sharma, P. and Kumar, S. (2001): Secondary metabolites of Artemisia annua and their biological activity. Current Science, 80: 35–48.
[18]
Malaisse, J. (1982): Alloxan toxicity to the pancreatic B-cell. Anew hypothesis. Biochem. Pharmacol, 31: 3527-3537.
[19]
Bancroft, D., and Gamble, M. (2008): Bancroft's Theory and Practice of Histological Techniques. 6th Ed. Elsevier, Churchill Livingst one, Edinburgh, Scotland, Pp 178-186, 221-224.
[20]
Hammer, Ø., Harper, D., Ryan, D. (2001): PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica., 4(1): 9-15.
[21]
Kim, N., Stankovic, M., Cushman, T., Goldstein, I., Munarriz, R. and Traish, M. (2006): Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers. BMC Physiol., 6:1–9.
[22]
Amaral, S., Mota, C., Lacerda, B., Alves, M., Pereira, Mde L., Oliveira, J. and Ramalho-Santos, J. (2009): Testicular mitochondrial alterations in untreated streptozotocin-induced diabetic rats. Mitochondrion, 9: 41–50.
[23]
Armagan, A., Uz, E., Yilmaz, R., Soyupek, S., Oksay, T. and Ozcelik, N. (2006): Effects of melatonin on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rat testis. Asian J Androl., 8: 595–600.
[24]
Amaral, S., Oliveira, J. and Ramalho-Santos, J. (2008): Diabetes and the impairment of reproductive function: Possible role of mitochondria and reactive oxygen species. Curr Diabetes Rev., 4: 46–54.
[25]
Annunziata, L., Domenico, F. and Pietro, T. (2005): Glyco-oxidation in diabetes and related diseases. Clin Chim Acta, 2: 236–250.
[26]
Bhasin, S., Enzlin, P., Coviello, A., and Basson, R. (2007): Sexual dysfunction in men and women with endocrine disorders. The Lancet, 369(9561): 597-611.
[27]
Seethalakshmi, L., Menon, M. and Diamond, D. (1987): The effect of streptozotocin-induced diabetes on the neuroendocrine-male reproductive tract axis of the adult rat. J Urol., 138: 190–194.
[28]
Cameron, F., Murray, T. and Drylie, D. (1985): Interstitial compartment pathology and spermatogenic disruption in testes from impotent diabetic men. Anat Rec., 213: 53–62.
[29]
Sadik, H., El-Seweidy, M. and Shaker, G. (2011): The antiapoptotic effects of sulphurous mineral water and sodium hydrosulphide on diabetic rat testes. Cell PhysiolBiochem, 28: 887–898.
[30]
Anderson, E. and Thliveris, A. (1986): Testicular histology in streptozotocin-induced diabetes. Anat Rec., 214: 378-382.
[31]
Koh, O (2007): Streptozotocin-induced diabetes increases apoptosis through JNK phosphorylation and Bax activation in rat testes. J Vet Med Sci., 69: 969–971.
[32]
Cai, L., Hales, F. and Robaire, B. (1997): Induction of apoptosis in the germ cells of adult male rats after exposure to cyclophosphamide. BiolReprod, 56: 1490–1497.
[33]
Samad, A., Shams, S., Ullah, Z., Wais, M., Nazish, I., Sultana, Y. and Aqil, M. (2009): Status of herbal medicines in the treatment of diabetes. Curr. Diabetes Rev., 5: 102-111
[34]
Yang, H., Jin, X., Kei Lam, W. and Yan, K. (2011): Oxidative stress and diabetes mellitus. Clinical Chemistry & Laboratory Medicine, 49(11): 1773-1782.
[35]
Bakkali, F., Averbeck, S., Averbeck, D. And Idaomar, M (2008): Biological effects of essential oils. Food Chem. toxicol, 46: 446-475.
