Thrombolytic Activity Profiling of Cassava, Manihot Esculenta Leaves and Tuber Extracts with Two Formulations
This study was aimed at in vitro thrombolytic activities of leaves and tuber extracts of cassava, Manihot esculenta. The color strength or the precipitate formation was used as analytical answers to these tests. An in vitro thrombolytic model was used to check the clot lysis effect of cassava leaves and tuber extracts with 400µg/ml and 800µg/ml of formulations using streptokinase as a positive control and water as a negative control. Utilizing in vitro model of thrombolytic activity assessment, the clot lysis value of 14.94±0.045 %, 10.43±0.035 % and 16.23±0.055 %, 20.656±0.056 % respectively of 400 µg/ml and 800 µg/ml formulations of cassava leaves and tubers extracts were documented in 72 hrs incubations. On the other hand, 3.91±0.025 % and 86.31±0.03 % clot lysis activity were acknowledged respectively in negative control (dH2O) and positive control (SK) in 72 hrs incubations. The mean differences in clot lysis percentage of Streptokinase (100 µl, 30,000.0 IU), 400µg/ml and 800µg/ml of cassava leaves and tubers extracts with negative control were significant (p < 0.001, H0 is rejected). In our study it was found that cassava, Manihot esculenta leaves and tubers possesses thrombolytic properties, however, in vivo clot dissolving properties and active component(s) of this plant for clot lysis are yet to be revealed. So, the appropriate amount of Manihot esculenta leaves and tubers could be integrated as a thrombolytic agent for the get better of patients suffering from atherothrombotic diseases
Cassava, Clot lysis, Atherothrombotic Diseases, Streptokinase
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