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Antibacterial Activity of Aqueous and Ethanolic Extracts of Leaf and Stem Bark of Vitex doniana on Staphylococcus aureus
Current Issue
Volume 6, 2018
Issue 3 (September)
Pages: 71-77   |   Vol. 6, No. 3, September 2018   |   Follow on         
Paper in PDF Downloads: 34   Since Jul. 24, 2018 Views: 1173   Since Jul. 24, 2018
Authors
[1]
Ubandoma Andefiki, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Taraba State, Nigeria.
[2]
Imarenezor Edobor Peter Kenneth, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Taraba State, Nigeria.
[3]
Brown Samuel Tamunoiyowuna Cockeye, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Taraba State, Nigeria.
[4]
Aregbe Elizabeth Adenike, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria.
[5]
Simon Shedrach, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari, Taraba State, Nigeria.
Abstract
The aim of this study titled “antibacterial activity of aqueous and ethanolic extracts of leaf and stem bark of Vitex doniana on Staphylococcus aureus” was to verify the pharmacological property of the plant for possible use in the remedy of diseases especially against antibiotic-resistant agents. It is widely suggested that a vast array of plants serve medicinal purposes without scientific proofs. Materials used for the study include culture media, spectrophotometer and other reagents. The plant sample was collected from within the environment of Federal University Wukari before the extraction of its materials using the cold maceration method. Phytochemical screening was done with the plant being found to contain bioactive substances such as alkaloid, flavonoid, tannins, cardiac glycosides, resins terpenes and steroids. The pH of both leaf and stem bark extracts was between 5 and 7. The extracts of the stem bark had minimum inhibitory concentration (MIC) of 4000 μg/ml on S. aureus while the leaf extracts had MIC greater than 4000 μg/ml. From the spectrophotometric absorbance, it was seen that different concentrations of extracts had different degrees of antibacterial activity on the test organism. This implies that V. doniana has antibacterial activity on S. aureus and therefore suggests it can be used as an alternative remedy for treating bacterial diseases.
Keywords
Vitex doniana, Staphylococcus aureus, Antibacterial, Leaf and Stem Bark
Reference
[1]
Mustapha, T. A., Abdurahaman, I. F., Khan Z. I. and Sandabe, U. K. (2012). The effects of ethanolic extract of Vitex doniana stem bark on peripheral and central nervous system of laborotory animals. Journal of Applied Pharmaceutical Science 02 (03): 74-79.
[2]
Ali, M., Aminu, F. and Ibrahim, I. S. (2017). In-vitro assessment of antibacterial activity and phytochemical screening of Vitex doniana on clinical isolate of Salmonella typhi. International Journal of Advanced Academic Research Science, Technology & Engineering. 3: 9-1.
[3]
Dauda, B. E. N., Oyeleke, S. B., Jigam, A. A., Salihu, S. O. and Balogun, M. M. (2011). Phytochemical and in-vitro antibacterial investigation of Vitex doniana leaves, stem bark and root bark extracts. Australian Journal of Basic and Applied Science 5 (7): 523-528.
[4]
Harley, R. M., Atkins, S., Budantsev, A., Cantino, P. D., Conn, B. J., Grayer, R. J., Harley, M. M., de Kok., R. P., Krestovskaja, T. V., Morales, R., Paton, A. J. and Ryding, P. O. (2004). “Labiatae” The families and Genera of Vascular Plants. Springer-VerlagBerlin, Heidelberg. 7: 167-275.
[5]
Orwa, C. A., Mutua, Kindt, R., Jamnadass, R. S. and Anthony (2009). Agroforestree http://www.worldagroforestry.org/sites/treedbs/treedatabases.asp
[6]
Bonquet, A. and Paris, R. (1991) Plantes medicinales du Congo-Brazzavile (III) plantes medicinales et phytotherapie 2: 154-158.
[7]
Kilani, A. M. (2006). Antibacterial assessment of whole stem bark of Vitex doniana against some enterobactriaceae. African Journal of Biotechnology. 5 (10): 958-959.
[8]
Yakubu, O. E., Nwodo, C. F. O. and Imarenezor, E. P. K. (2016). Nephroprotective effects of leaves of Vitex doniana aqueous extract on streptozotocin-induced diabetes rats. FUW Trends in science & Technology Journal. 1: 288-291.
[9]
Vunchi, A. M., Umar, N. A., King, A. M., Liman, A. A., Jeremiah, G. and Aigbe O. C. (2011). Proximate, vitamins and mineral composition of Vitex doniana (Black Plum) Fruit Pulp: Journal of Basic and Applied Science. 19 (1): 97-101.
[10]
Singleton, P. and Sainsbury, D. (2006). Dictionary of Microbiology and Molecular Biology 3th edition. John Wiley and Sons, Ltd. The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England. 236, 423, 616, 621, 624.
[11]
Kayser, H. F., Bienz A. K., Eckert, J. and Zinkernagel M. R. (2005). Medical Microbiology. Georg Thieme Verlag, Ru¨ digerstraße 14, 70469 Stuttgart, Germany.
[12]
Bhatia, R. and Icchpujani, R. L. (2008). Essential of Medical Microbiology, 4th edition Jaypee Brothers Medical Publishers (JPBMP) Ltd B-3 EMCA Hons 23/23B Ausari Road Daryaganj.
[13]
Stuart, H. (2005). Essential Microbiology, John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England Ltd. 169, 365, 176.
[14]
Vadivu, R., Krithika, A., Biphas, C., Dedeopya, P., Shoeb, K. S., and Lakshmi, K. S. (2008). Evaluation of haptoprotective activity of the fruits of Coccina grandis Lim. International journal of Health Research. 3: 163-168.
[15]
Stewart, C. M. (2003). Staphylococcus aureus and Staphylococcus enterotoxins. Ch 12 in Hockin AD (ed) foodborne microorganism of public health significance 6th ed. Australia institute of food science and technology (NSW), Sydney, P. 359-380.
[16]
Osuagwu, G. G. E. and Eme, C. F. (2013). The phytochemical composition and antimicrobial activity of Dialium guineense, Vitex doniana and Dennettia tripetala Leaves. Asian Journal Of Natural & Applied Sciences. 2 (3): 169-181.
[17]
Shahid-Ud-Daula, A. F. M. And Mohammad, A. B., (2009). Phytochemical Screening, Plant Growth Inhibition, And Antimicrobial Activity Studies Of Xylocarpus GranatumMalaysian Journal Pharmaceutical Sciences, 7: 9–21.
[18]
Ubandoma, A. Inabo, H. I. Abdullahi, I (2017). In Vivo Activity of Fractions of Combretum Molle R. and Haematological Profile of Trypanosoma Brucei Brucei Infected Mice. American Journal of Pharmacy and Pharmacology, 4 (4) 15-20.
[19]
Dawang N. D. (2015). Phytochemical Constituents And Toxicological Study Of Vitex Doniana LeafJournal of Pharmacy and Biological Sciences 10: 23-27.
[20]
Ugbogu, O. C. Okereke, H. C. kanu, I. J. Nwachukwu, N. C. Onwuchekwa, E. C. Ogbu, O. Onyeagba, R. A. Umeham, S. N. Azu, N. C. Orji, J. Umezuruike, E. Nworie, O. Amadi, M. I. Nwachukwu, M. I (2007). Laboratory guide for Microbiology. Crystal publishers 49., Owerri road, Okigew Imo State. 33-38, 192-201.
[21]
Nwachukwu, E., and Uzoeto, O. H., (2009) Antimicrobial Activities Of Leaf Of Vitex Doniana and Cajanus Cajan On Some Bacteria. Researcher 2 (3): 37-47.
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