Antibacterial Activity of Aqueous and Ethanolic Extracts of Leaf and Stem Bark of Vitex doniana on Staphylococcus aureus
The aim of this study titled “antibacterial activity of aqueous and ethanolic extracts of leaf and stem bark of Vitex doniana on Staphylococcus aureus” was to verify the pharmacological property of the plant for possible use in the remedy of diseases especially against antibiotic-resistant agents. It is widely suggested that a vast array of plants serve medicinal purposes without scientific proofs. Materials used for the study include culture media, spectrophotometer and other reagents. The plant sample was collected from within the environment of Federal University Wukari before the extraction of its materials using the cold maceration method. Phytochemical screening was done with the plant being found to contain bioactive substances such as alkaloid, flavonoid, tannins, cardiac glycosides, resins terpenes and steroids. The pH of both leaf and stem bark extracts was between 5 and 7. The extracts of the stem bark had minimum inhibitory concentration (MIC) of 4000 μg/ml on S. aureus while the leaf extracts had MIC greater than 4000 μg/ml. From the spectrophotometric absorbance, it was seen that different concentrations of extracts had different degrees of antibacterial activity on the test organism. This implies that V. doniana has antibacterial activity on S. aureus and therefore suggests it can be used as an alternative remedy for treating bacterial diseases.
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