Simple UV Spectrophotometric Assay of Glimepride
Glimepride belongs to sulfonylurea oral anti diabetic. A efficient least time consuming and simple spectrophotometric method for the assay of Glimepride has been used. The assay is based on the ultraviolet UV absorbance maxima at about 200nm wavelength of Glimepride using water as solvent. A sample of drug was dissolved in water to produce a solution containing Glimepride. Similarly, various dilutions were made. The absorbance of sample preparation was measured at 200nm against the solvent blank and the assay was determined. In our study a simple and quick assay method using U.V spectrophotometer has been used. The assay is based on measuring the absorbance of formulation of Glimepride dilutions at the wavelength of 200 nm. Four different dilutions of 50ppm, 25ppm, 12.5ppm and 6.25ppm is prepared and their percent assay is calculated.
Khedekar PB, Dhole SM, Bhusari KP (2010), Application of vierodt’s andabsorption correction spectrophotometric methods for estimationof rosiglitazone maleate and glimepiride in tablets, Digest Journalof Nanomaterial and Biostructures, 5(1), 77-84.
Lakshmi KS, Rajesh T, Sharma S, Lakshmi S (2009) Development and validation of liquid chromatographic and UV derivative spectrophotometric methods for the determination of metformin, pioglitazone and glimepiride in pharmaceutical formulations, Der Pharma Chemica, 1(1), 238-46.
Lehr, K. H., & Damm, P. (1990). Simultaneous determination of the sulphonylurea glimepiride and its metabolites in human serum and urine by high-performance liquid chromatography after pre-column derivatization. Journal of Chromatography B: Biomedical Sciences and Applications, 526, 497-505.
Khan, M. A., Sinha, S., Vartak, S., Bhartiya, A., & Kumar, S. (2005). LC determination of glimepiride and its related impurities. Journal of pharmaceutical and biomedical analysis, 39(5), 928-943.
Sane, R. T., Menon, S. N., Inamdar, S., Mote, M., & Gundi, G. (2004). Simultaneous determination of pioglitazone and glimepiride by high-performance liquid chromatography. Chromatographia, 59(7-8), 451-453.
Rabbaa‐Khabbaz, L., Abi Daoud, R., Karam‐Sarkis, D., Atallah, C., & Zoghbi, A. (2005). A simple and sensitive method for determination of glimepiride in human serum by HPLC. Journal of liquid chromatography & related technologies, 28(20), 3255-3263.
Altinöz, S., & Tekeli, D. (2001). Analysis of glimepiride by using derivative UV spectrophotometric method. Journal of pharmaceutical and biomedical analysis, 24(3), 507-515.
Sane, R. T., Menon, S. N., Inamdar, S., Mote, M., & Menezes, A. (2004). Simultaneous determination of pioglitazone and glimepiride by high-performance thin-layer chromatography. JPC-Journal of Planar Chromatography-Modern TLC,17(2), 154-156.
Huma Dilshad, Safila Naveed and Ghulam Sarwar (2014) Simple spectrophotometric assay of available brands of Acetaminophen tablets and their comparative study Journal of pharmacy and pharmaceutical sciences Volume 2, Issue 1,1-4
Safila Naveed, Fatima Qamar, Ghulam Sarwer., (2014) Percentage assay of metformin in different medium using UV- spectrophotometer BPJ0000130 - World Research Journal of Organic Chemistry Volume : 2 Issue : 1, 12-14 ISSN: 2320-3374 & E-ISSN: 2320-5679, http://www.bioinfopublication.org/jouarchive.php?opt=&jouid=BPJ0000130
Safila Naveed (2014) Simple UV spectrophotometric assay of Atorvastatin API formulation and their comparative study, Global Journal of Medical Research .14(2):35-38. https://globaljournals.org/GJMR_Volume14/4-Simple-UV-Spectrophotometric-Assay.pdf
Safila Naveed and Fatima Qamar (2014) A simple assay of Esomeprazole Using UV spectrophotometer The Global Journal of Pharmaceutical Research (TGJPR) 3(2); 1921-25, http://www.tgjpr.com/view-article.php?id=3433
Safila Naveed and Fatima Qamar (2014) Simple UV spectrophotometric assay of Mefenamic acid International Journal of Pharma Sciences and Research (IJPSR) ,5(7):364-366 .http://www.ijpsr.info/ijpsr-v5n7.php
Safila Naveed, Shabana Naz Shah, Fatima Qamar , Nimra Waheed, and Safeena Nazeer, (2014) simple uv spectrophotometric assay of new formulation gentamycin J App Pharm Vol. 6; Issue 4: 407-410
Safila Naveed, Shabana Naz Shah, Fatima Qamar , Nimra Waheed, and Safeena Nazeer, (2014) Simple UV spectrophotometric assay of Lincomycin, IJPRDD, 1 (2),10-12.
Safila Naveed* and Amber Nawab (2014) Assay of LVFX (levofloxacin) in different formulation by UV spectroscopy IJPRDD, 1 (2), 13-16.
Safila Naveed and Fatima Qamar (2014) Simple UV spectrophotometric assay of Metronidazole Open Access Library Journal, 1: e615:1-4. http://dx.doi.org/10.4236/oalib.1100615
Huma Dilshad, Safila Naveed and Baqir Naqvi (2013) Assay of new formulations of isosorbide mononitrate by using uv spectrophotometer : BPJ0000115 - World Research Journal of Medicine Volume : 1 Issue : 1, pg9-10.