[1]
Orhue Philips Ogbeide, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
[2]
Momoh l Abdul-Razaq McSione, Department of Medical Microbiology, Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
Different solvents are known to present different extraction potency for plants and their active constituents and metabolites and for this reason, when used for antibiotic extraction, different inhibitory activities and potency should be expected. This study evaluates the antibacterial potential of different extracting solvents of Citrus aurantifolia leave against some infection causing microorganisms. In a bid to evaluate the aim of this study, 5 grams of dried blended leaves of Citrus aurantifolia was mixed with 95ml of six different extraction solvents (Hydrochloric acid, Propylene glycol, Hot water, Acetic acid, Acetone and Ethanol) for 24 hours. The constituted filter extracts were aseptically assessed and their minimum inhibitory concentration (MIC) on different miro-organism (Pseudomonas aeruginosa, Escherichiia coli, Staphylococcus aureus and Klebsiella spp) were determined. The results showed that hot water extraction of Citrus aurantifolia leave has no effect on the herein studied micro organisms. However, ethanol extract of Citrus aurantifolia leave was the most sensitive to the micro-organisms but Klebsiella spp was 100% resistant. Acetic acid, Propylene glycol and acetol extracts were sensitive to all the micro-organism at different MICs. Based on these findings, it is suggested that solvent extraction potency be investigated for plants known or acclaimed to have antibacterial activity.
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