Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News
The Antimicrobial Activities of Citrus Aurantifolia Leave Extracts Against Some Bacteria
Current Issue
Volume 7, 2019
Issue 2 (June)
Pages: 32-35   |   Vol. 7, No. 2, June 2019   |   Follow on         
Paper in PDF Downloads: 36   Since May 28, 2019 Views: 1136   Since May 28, 2019
Orhue Philips Ogbeide, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
Momoh l Abdul-Razaq McSione, Department of Medical Microbiology, Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
Different solvents are known to present different extraction potency for plants and their active constituents and metabolites and for this reason, when used for antibiotic extraction, different inhibitory activities and potency should be expected. This study evaluates the antibacterial potential of different extracting solvents of Citrus aurantifolia leave against some infection causing microorganisms. In a bid to evaluate the aim of this study, 5 grams of dried blended leaves of Citrus aurantifolia was mixed with 95ml of six different extraction solvents (Hydrochloric acid, Propylene glycol, Hot water, Acetic acid, Acetone and Ethanol) for 24 hours. The constituted filter extracts were aseptically assessed and their minimum inhibitory concentration (MIC) on different miro-organism (Pseudomonas aeruginosa, Escherichiia coli, Staphylococcus aureus and Klebsiella spp) were determined. The results showed that hot water extraction of Citrus aurantifolia leave has no effect on the herein studied micro organisms. However, ethanol extract of Citrus aurantifolia leave was the most sensitive to the micro-organisms but Klebsiella spp was 100% resistant. Acetic acid, Propylene glycol and acetol extracts were sensitive to all the micro-organism at different MICs. Based on these findings, it is suggested that solvent extraction potency be investigated for plants known or acclaimed to have antibacterial activity.
Antibacterial, Plant Extracts, Extracting Solvent, Citrus aurantifolia Leave
Onyeagba, R., Ugbogu, O., Okeke, C. and Iroakasi, O. 2005. Studies on the antimicrobial effects of garlic (Allium sativum Linn), ginger (Zingiber officinale Roscoe) and lime (Citrus aurantifolia Linn). African Journal of Biotechnology 3 (10): 552-554.
Nkambule, T. P. 2008. Antimicrobial Properties of Selected Asian Herbs. Master Thesis. University of Florida.
Amrita, V., Sonal, D. and Shalini, R. 2009. Antibacterial Effect of Herbs and Spices Extract on Escherichia coli. Electronic Journal of Biology 5 (2): 40-44.
Aboaba, O., Smith, S. and Olude, F. 2006. Antibacterial effect of edible plant extract on Escherichia coli O157: H7. Pakistan Journal of Nutrition 5 (4): 325-327.
Iqbal Ahmad, Arina Z. Beg. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of Ethnopharmacology. 2001; 74; 113-123.
Pathan R, Papi R, Parveen P, Tananki G, Soujanya P. In vitro antimicrobial activity of Citrus aurantifolia and its phytochemical screening. Asian Pac J Trop Dis. 2012; 2: 328–31.
Indu, M., Hatha, A., Abirosh, C., Harsha, U. and Vivekanandan, G. 2006. Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Brazilian Journal of Microbiology 37 (2): 153-158.
Mohanta, T., Patra, J., Rath, S., Pal, D. and Thatoi, H. 2007. Evaluation of antimicrobial activity and phytochemical screening of oils and nuts of Semicarpus anacardium. Scientific Research and Essay 2 (11): 486-490.
Hema, R., Kumaravel, S. and Elanchezhiyan, N. 2009. Antimicrobial Activity of Some of the South-Indian Spices and Herbals against Food Pathogens. Global Journal of Pharmacology 3 (1): 38-40.
Tagoe, D., Baidoo, S., Dadzie, I., Kangah, V. and Nyarko, H. 2010. A comparison of the antimicrobial (antifungal) properties of garlic, ginger and lime on Aspergillus flavus, Aspergillus niger and Cladosporium herbarum using organic and water base extraction methods. The Internet Journal of Tropical Medicine 7 (1): DOI: 10.5580/1099.
Yan, S. and Asmah, R. 2010. Comparison of total phenolic contents and antioxidant activities of turmeric leaf, pandan leaf and torch ginger flower. International Food Research Journal 17: 417-423.
Ibukun, A., Tayo, A., Toyin, A, Tolu, O. (2007). Evaluation of the Antimicrobial properties of Different parts of Citrusaurantifolia (Lime Fruit) as used Locally. Afri J Tradt Complement Altern Med, 4 (2): 185-190.
Khan, P. R., Gali, P. R., Pathan, P., Gowthan, T., Pasupuleti, S. (2012). In Vitro antimicrobial activity of Citrus aurantifoliaand its phytochemical screening. Live Sciences feed, 1 (2): 13 – 16.
Onyeagba, R. A, Ugbogu, O. C, Okeke, C. U. Iroakasi, O. (2004). Studies on the antimicrobial effects of garlic (Alliumsativum Linn), ginger (Zingiber officinale Roscoe) and Lime (Citrus aurantifolia Linn). Afri J Biotechnol, 3 (10): 552–554.
Nwankwo IU, Osaro-Matthew RC, Ekpe IN. Synergistic antibacterial potentials of Citrus aurantifolia (Lime) and honey against some bacteria isolated from sputum of patients attending Federal Medical Center, Umuahia. Int J Curr Microbiol Appl Sci. 2015; 4: 534–44.
Aibinu I, Adenipekun T, Adelowotan T, Ogunsanya T, Odugbemi T. Evaluation of the antimicrobial properties of different parts of Citrus aurantifolia (lime fruit) as used locally. Afr J Tradit Complement Altern Med. 2006; 4: 185–90.
Balamurugan S. In vitro antifungal activity of Citrus aurantifolia Linn plant extracts against phytopathogenic fungi Macrophomina phaseolina. Int Lett Nat Sci. 2014; 13: 70–4.
Dongmo PJ, Tatsadjieu L, Sonwa ET, Kuate J, Amvam Zollo P, Menut C. Essential oils of Citrus aurantifolia from Cameroon and their antifungal activity against Phaeoramularia angolensis. Afr J Agric Res. 2009; 4: 354–8.
Tagoe D, Baidoo S, Dadzie I, Kangah V, Nyarko H. A comparison of the antimicrobial (antifungal) properties of garlic, ginger and lime on Aspergillus flavus, Aspergillus niger and Cladosporium herbarum using organic and water base extraction methods. Int J Trop Med. 2009; 7: 1–8.
Domínguez-Vigil I, Camacho-Corona M, Heredia-Rojas J, Vargas-Villarreal J, Rodríguez-De la Fuente A, Heredia-Rodríguez O, et al. Anti-giardia activity of hexane extract of Citrus aurantifolia (Christim) Swingle and some of its constituents. Afr J Tradit Complement Altern Med. 2015; 12: 55–9.
Enejoh S, Suleiman M, Ajanusi J, Ambali S. In vitro anthelmintic efficacy of extracts of Citrus aurantifolia (Christm) Swingle fruit peels against Heligmosomoides bakeri ova and larvae. Int J Curr Pharm Res. 2015; 7: 92–6.
Enejoh S, Shuaibu K, Suleiman M, Ajanusi J. Evaluation of anthelmintic efficacy of extracts of Citrus aurantifolia fruit juice in mice experimentally infected with Heligmosomoides bakeri. Int J Biol Res. 2014; 4: 241–6.
Abdelqader A, Qarallah B, Al-Ramamneh D, Das G. Anthelmintic effects of Citrus peels ethanolic extracts against Ascaridia galli. Vet Parasitol. 2012; 188: 78–84.
Soonwera M. Efficacy of essential oils from Citrus plants against mosquito vectors Aedes aegypti (Linn.) and Culex quinquefasciatus (Say) J Agric Technol. 2015; 11: 669–81.
Effiom O, Avoaja D, Ohaeri C. Mosquito repellent activity of phytochemical extracts from fruit peels of Citrus fruit species. Glob J Sci Front Res Interdiscip. 2012; 12: 5–8.
Orhue, P. O. (2004). Antibiogram types of urinary tract infection bacteria isolates and the susceptibility to some indigenous plant extract. Ph.D Thesis, Ambrose Alli University, Ekpoma, Nigeria.
Dugo G., Cotroneo A., Bonaccorsi I. and Trozzi A., Composition of the volatile fraction of Citrus peel oils. In Citrus oils – Composition, advanced analytical techniques, contaminants, and biological activity. Edits. G. Dugoand L. Mondello, pp. 1–161, CRC Press, Boca Raton, FL (2011).
Costa R, Bisignano C, Filocamo A, Grasso E, Occhiuto F and Spadaro F. (2014) Antimicrobial activity and chemical composition of Citrus aurantifolia (Christm.) Swingle essential oil from Italian organic crops, Journal of Essential Oil Research, 26: 6, 400-408.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
Copyright © 2013-, Open Science Publishers - All Rights Reserved