The Cytogenetic Effects Evaluation of Non-thermal Radiofrequency Radiation from Cellular Phones on Rat Peripheral Blood Lymphocytes
[1]
El Idrissi Sidi Brahim Salem, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco; Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[2]
El Arbi Boussaber, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
[3]
El Goumi Younes, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
[4]
Hayat Talbi, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
[5]
Choukri Abdelmajid, Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[6]
Hillali Abderraouf, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
The widespread use of mobile phones over the past decade has raised considerable concern public about possible biological effects of electromagnetic field emitted from cell phones and their consequences on human health. The aim of this study was to evaluate whether chronic whole-body exposure to non-thermal radiofrequency (RF) radiation from cellular phones could induce cytogenetic effects on peripheral blood lymphocytes of rats exposed to radiofrequency radiation. Twelve (23) Wistar rats were used for the study and randomly divided into 4 groups according to time of exposure, animals in treatment were exposed respectively for: oh (control), 1h, 2 and 3h daily 7 days a week for up 15 weeks to 900 MHz radiofrequency radiation at an average special absorption rate (SAR) of 0.873-0.352 W/kg, all rats were visually checked daily and were weighed weekly. After the period of exposure, blood samples were collected from all groups and peripheral blood cultures were performed using standard laboratory methods for the extent of genotoxicity, assessed by the cytokinesis-block micronucleus assay. The results indicated that significant increase of micronucleated (MN) cells in the lymphocytes among the study groups in a time-dependent manner compared with the control. The proliferation index (PI), is significantly decreased in all exposed groups in a time-dependent manner compared with the control, however body weight gain was insignificantly changed in all groups exposed comparing to unexposed rats. This study shows that the chronic exposure to 900 MHz radiofrequency radiation from cell phones may induce cytogenetic effects in rat lymphocytes, and this effect is more remarkable for long exposure time.
Rat Lymphocytes, Radiofrequency, Micronucleus Test, Proliferation Index, Body Weight Gain
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