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The Cytogenetic Effects Evaluation of Non-thermal Radiofrequency Radiation from Cellular Phones on Rat Peripheral Blood Lymphocytes
Current Issue
Volume 4, 2017
Issue 4 (August)
Pages: 20-28   |   Vol. 4, No. 4, August 2017   |   Follow on         
Paper in PDF Downloads: 27   Since Aug. 1, 2017 Views: 1157   Since Aug. 1, 2017
Authors
[1]
El Idrissi Sidi Brahim Salem, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco; Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[2]
El Arbi Boussaber, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
[3]
El Goumi Younes, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
[4]
Hayat Talbi, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
[5]
Choukri Abdelmajid, Laboratory “Polymers, Radiations and Environment”, Faculty of Sciences, University Ibn Tofail, Kenitra, Morocco.
[6]
Hillali Abderraouf, Laboratory of Agrofood and Health, Faculty of Sciences and Technology, University Hassan 1, Settat, Morocco.
Abstract
The widespread use of mobile phones over the past decade has raised considerable concern public about possible biological effects of electromagnetic field emitted from cell phones and their consequences on human health. The aim of this study was to evaluate whether chronic whole-body exposure to non-thermal radiofrequency (RF) radiation from cellular phones could induce cytogenetic effects on peripheral blood lymphocytes of rats exposed to radiofrequency radiation. Twelve (23) Wistar rats were used for the study and randomly divided into 4 groups according to time of exposure, animals in treatment were exposed respectively for: oh (control), 1h, 2 and 3h daily 7 days a week for up 15 weeks to 900 MHz radiofrequency radiation at an average special absorption rate (SAR) of 0.873-0.352 W/kg, all rats were visually checked daily and were weighed weekly. After the period of exposure, blood samples were collected from all groups and peripheral blood cultures were performed using standard laboratory methods for the extent of genotoxicity, assessed by the cytokinesis-block micronucleus assay. The results indicated that significant increase of micronucleated (MN) cells in the lymphocytes among the study groups in a time-dependent manner compared with the control. The proliferation index (PI), is significantly decreased in all exposed groups in a time-dependent manner compared with the control, however body weight gain was insignificantly changed in all groups exposed comparing to unexposed rats. This study shows that the chronic exposure to 900 MHz radiofrequency radiation from cell phones may induce cytogenetic effects in rat lymphocytes, and this effect is more remarkable for long exposure time.
Keywords
Rat Lymphocytes, Radiofrequency, Micronucleus Test, Proliferation Index, Body Weight Gain
Reference
[1]
Kanu Megha a, Pravin Suryakantrao Deshmukh a, Basu Dev Banerjee a,*, Ashok Kumar Tripathi a, Rafat Ahmed a, Mahesh Pandurang Abegaonkar b Low intensity microwave radiation induced oxidative stress, inflammatory response and DNA damage in rat brain. NeuroToxicology 51 (2015) 158–165.
[2]
Vecchia, P., Matthes, R., Ziegelberger, G., Lin, J., Saunders, R., Swerdlow, A. (2009) ‘Exposure to High Frequency Electromagnetic Fields, Biological Effects and Health Consequences (100 kHz-300 GHz)’ ICNIRP 16/2009.
[3]
Diem E, Schwarz C, Adlkofer F, Jahn O, et Rüdiger H, (2005). Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutât Res; 583: 178-183.
[4]
Hardell L, K H Mild, M Carlberg and F Söderqvist, (2006). Tumour risk associated with use of cellular telephones or cordless desktop telephones. World J Surg Oncol Oct 11; 4 (1): 74.
[5]
Zotti-Martelli L, Peccatori M, Maggini V, Ballardin M, et Barale R, (2005). Individual responsiveness to induction of micronuclei in human lymphocytes after exposure in vitro to 1800 MHz microwave radiation, Mutat Res., 582: 42-52.
[6]
Gandhi G, Kaur G, Nisar U (2014). A cross-sectional case control study on genetic damage in individuals residing in the vicinity of a mobile phone base station. Electromagn Biol Med. 34 (4): 344- 354.
[7]
Vijayalaxmi, Leal, B. Z., Szilagyi, M., Prihoda, T. J. and Meltz, M, (2000). Primary DNA damage in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation. Radiat Res; 153: 479-486.
[8]
Vijayalaxmi, Pickard, W. F., Bisht, K. S., Leal, B. Z., Meltz, M. L., Roti Roti, J. L., Straube, W. L. and Moros, E. G (2001). Cytogenetic studies in human blood lymphocytes exposed in vitro to radiofrequency radiation at a cellular telephone frequency (835.62 MHz, FDMA) Radiat Res; 155: 113-121.
[9]
Vijayalaxmi, (2006). Cytogenetics studies in human blood lymphocytes exposed in vitro to 2.45 GHz or 8.2 GHz radiofrequency radiation. Radiat Res; 166: 532-538.
[10]
Gos P, Eicher B, Kohli J, Heyer W-D, (2000). No mutagenic or recombinogenic effects of mobile phone fields at 900 MHz detected in the yeast Saccharomyces cerevisiae Bioelectromagnetics; 21: 515-523.
[11]
Roti Roti, J. L., Malyapa, R. S., Bisht, K. S., Ahern, E. W., Moros, E. G., Pickard, W. F., Straube, W. L., (2001). Neoplastic transformation in C3H 10T (1/2) cells after exposure to 835.62 MHz FDMA and 847.74 MHz CDMA radiations. Radiat. Res. 155, 239-247.
[12]
Maes A, Collier M, Verschaeve L (2001). Cytogenetic effects of 900 MHz (GSM) microwaves on human lymphocytes. Bioelectromagnetics; 22: 91-96.
[13]
Lagroye I, Anane R, Wettring BA, Moros EG, Straube WL, Laregina, Niehoff M, Pickard WF, Baty J, Roti Roti JL, (2004). Measurements of DNA damage after acute exposure to pulsed waves 2450MHz in rat brain cells by two alkaline comet assay methods. Int J Radiat Biol; 80 (1): 11-20.
[14]
Hook, G. J., Zhang, P., Lagroye, I., Li, L., Higashikubo, R., Moros, E. G., Straube, W. L., Pickard, W. F., Baty, J. D. and Roti Roti, J. L., (2004). Measurement of DNA damage and apoptosis in Molt-4 cells after in vitro exposure to radiofrequency radiation Radiat Res; 161: 193-200.
[15]
Zeni, O, Chiavoni AS, Sannino A, Antonili A, Forigo D, Bersani F, Scarfi MR, (2004). Lack of genotoxic effects (micronucleus induction) in human lymphocytes exposed in vitro at 99MHz EMF. Radiation Res; 160-2: 152-158.
[16]
Sakuma N, Komatsubara Y, Takeda H, Hirose H, Sekijima M, Nojima T, Miyakoshi J, (2006). DNA strand breaks are not induced in human cells exposed to 2.1425 GHz band CW and W-CDMA modulated radiofrequency fields allocated to mobile radio base stations. Bioelectromagnetics; 27: 51-57.
[17]
Stronati L, Testa A, Moquet J, Edwards A, Cordelli E, Villani P, Marino C, Fresegna AM, Appolloni M, Lloyd D, (2006).935 MHz cellular phone radiation. An in vitro study of genotoxicity in human lymphocytes. Int J Radiat Biol; 82: 339-346.
[18]
Glaser Katharina, Martina Rohland, Thomas Kleine-Ostmann, Thorsten Schrader, Helga Stopper, Henning Hintzsche, (2016). Effect of Radiofrequency Radiation on Human Hematopoietic Stem Cells, Radiation Research; Vol. 186, No. 5, pp. 455-465.
[19]
Liling Su, Xiaoxia Wei, Zhengping Xu, (2017). Guangdi Chen. RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells. Bioelectromagnetics, 38 (3): 175-185.
[20]
Fenech M, Morley AA (1985). Measurement of micronuclei in lymphocytes. Mutat Res; 147: 29–36.
[21]
Fenech, M. 1993. The cytokinesis – block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in a human population. Mutat. Res., 285: 35-44.
[22]
Titenko-Holland, N., Windhan, G., Kolachana, P., Reinish, F., Paravatham, S., Osorio, A. M. and Smith, M. T, (1997). Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mut. Res., 388: 85-95.
[23]
Jin, Y. B., Lee, H. J., Seon Lee, J., Pack, J. K., Kim, N., Lee, Y. S., 2010 Dec 21. One-year, simultaneous combined exposure of CDMA and WCDMA radiofrequency electromagnetic fields to rats. Int. J. Radiat. Biol. [Epub ahead of print].
[24]
Sonmez OF, Odaci E, Bas O, Kaplan S. Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz electromagnetic field. Brain Res 2010; 1356: 95–101.
[25]
Grafstrom, G. et al., Histopathological examinations of rat brains after long-term exposure to GSM-900 mobile phone radiation, Brain Res. Bull. 5 (2008) 257–26.
[26]
Ray, S., & Behari, J., (1990). Physiological changes in rats after exposure to low levels of microwaves. Radiat Res 123: 190-202.
[27]
Krstic DD, Dinctic BJ, Sokolovic DT, Markovic VV, Petkovic DM, Radic SB, (2005). The results of experimental exposition of mice by mobile telephones. Mikrotalasna revija; 11: 34-37.
[28]
Hugo W. Ruediger, (2009). Genotoxic effects of radiofrequency electromagnetic fields Pathophysiology 16 89–102.
[29]
Sekeroglu Vedat, Aysegul Akar, Zulal Atlı Sekeroglu, (2012). Cytotoxic and genotoxic effects of high-frequency electromagnetic fields (GSM 1800 MHz) on immature and mature rats Ecotoxicology and Environmental Safety 80 140–144.
[30]
Trosic I, Busljeta I, Modlic B, (2004). Investigation of the genotoxic effect of microwave irradiation in rat bone marrow cells: in vivo exposure. Mutagenesis. 19 (5): 361-364.
[31]
Balode, Z, (1996). Assessment of radio-frequency electromagnetic radiation by themicronucleus test in bovine peripheral erythrocytes. Science of the Total Environment, 180 (1): 81-85.
[32]
Vijayalaxmi, L. B. Sasser, J. E. Morris, B. W. Wilson, L. E. Anderson, (2003). Genotoxic potential of 1.6 GHz wireless communication signal: in vivo two-year bioassay, Radiat. Res. 159 (4) 558–564.
[33]
Scarfi, M. R., A. M. Fresegna, P. Villani, R. Pinto, C. Marino, M. Sarti, A. Sannino, P. Altavista, and G. A. Lovisolo, (2006). Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes. Radiat. Res 165: 655–663.
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