Skin Depigmentation Profiles of Ethanolic Fraction of the South African “Green” Cyclopia intermedia and Other Related Biological Potentials
[1]
Olugbenga Kayode Popoola, Department of Chemistry, Ekiti State University, Ado-Ekiti, Nigeria.
Background: The process of aging in human is complex with underlying multiple influences including the probable involvement of free radicals and varying degree of environmental factors. Over accumulation of such free radicals and other related factors resulted into cellular oxidative damage to important macromolecules such as proteins, lipids and deoxyribonucleic acid eventually leading to many chronic diseases like cancer, diabetes, premature skin aging, atherosclerosis and neurodegenerative disorders. Cyclopia intermedia (honeybush) is notable in South Africa and globally for medicinal (including alleviate heartburn and nausea, stimulate milk-production in breast-feeding women and treat colic in babies) and economic importance such as in worldwide production of tea and beverages. This study is therefore directed towards searching for biological values of honeybush in the area of possible combating with cellular oxidative stress and alleviating early skin aging. Method: An ethanolic extract of the South African fynbos species Cyclopia intermedia was successively fractionated in an open column of silica using a gradient of hexane: ethyl acetate. Phytochemical constituents of the most bioactive column fraction (VIII) was profiled using chromatographic and LC-ESI-MS methods with notable phenolics as well as unidentified analogues thereof. Result: The biological profile of fraction VIII assessed using colorimetric assays gave potent anti-tyrosinase activity IC50 28.125 µg/mL, while the in vitro total antioxidant capacities showed VIII with ORAC (3218.72 ± 90.14 µmol TE/g); TEAC (9903.21 ± 161.73 µmol TE/g); FRAP (6146.38 ± 5.72 µmol AAE/g) and iron (II)-induced microsomal lipid peroxidation estimated as IC50 260.173 µg/mL. Conclusion: The outcome of this investigation suggested more unexplored phenolics are still in existence in C. intermedia with possible correlation between the anti-tyrosinase of fraction VIII to the total antioxidant capacities demonstrated.
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