Synthesis and Biological Evaluation of Two Oxidovanadium (IV) Complexes as DNA-binding and Apoptosis-Inducing Agents
[1]
Zhenfeng Ye, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, People′s Republic of China.
[2]
Gaojian Yan, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People’s Republic of China.
[3]
Jing Tang, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, People′s Republic of China.
[4]
Qiangqiang Fu, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, People′s Republic of China.
[5]
Jiazheng Lu, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, People′s Republic of China.
[6]
Ning Yang, Department of Clinical pathology, the First Affiliated Hospital, Guangdong Pharmaceutical University, Guangzhou, People’s Republic of China.
Two Schiff-base azo compounds and their corresponding oxidovanadium (IV) complexes [VO(astsc)(p-CF3PIP)] (1) and [VO(astsc)(p-BPIP)] (2) [astsc=5-(4-Methyl Phenyl)-Azo-Salicylaldehyde Thiosemicarbazone, p-CF3PIP=2-(4-trifluoromethyl phenyl)imidazole[4,5-f][1,10]phenanthroline, and p-BPIP=2-(4-bromine phenyl imidazole [4,5-f][1,10] phenanthroline] have been synthesized and characterized by elemental analysis, UV–vis, ESI-MS, IR, 1H NMR and 13C NMR. The interaction of these two complexes with calf thymus DNA (CT-DNA) was investigated by absorption titration, fluorescence spectra and viscosity measurements. The cytotoxicity of the two complexes against Hela cancer cell lines was tested by MTT assay. The results suggest that both 1 and 2 bind to CT-DNA via an intercalative mode, and the DNA-binding affinity of 1 is smaller than that of 2. Complex 1 has more potent inhibitory effect than that of 2, and it obviously could induce cancer cells early apoptosis.
Schiff-Base, Oxidovanadium Complexes, DNA-bindng, Cytotoxicity, Apoptosis
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