Evaluation of Cell Suspension Chemical Profile of Iranian Fennel by TLC and GC-MS

Maziar Bahreini; Fereydoon Bondarian; Majid Gholibegian; Kambiz Larijani

doi:7120191

Etheric oil of Apiacea family has notable importance in terms of health, and synthesis of pharmaceutical and cosmetics materials. Fennel (foeniculum vulgare) has been known as Mediterranean aroma plant and its volatile oil uses for anti-microbial, anti-fungal and favoring applications. The subject of this work was analysis of metabolite accumulation of calli which were suspended in darkness condition that isolated by n-Hexane, cell suspension has been made by ½ Murashige & Skoog (MS) with 2 mg l^-1 2,4-D and 0.25 mg l^-1 BAP as phytohorrmone combinations, semi quantitative analysis was carried out by TLC and Identification of extract constituents was done by GC-MS by calculation of Kovats indices using Homologous series of n-alkanes C₈ -C₁₉. In conclusion Decane was detected as major compound and other constituents were cyclic and acyclic hydrocarbons.

[1]

Adams. RP (2002) Identification of essential oil components by gas chromatography mass spectrometry. Illinois: Allured Publishing Corporation.

[2]

Anubhuti, Pasrija, Rahul, Singh, Chandra Kant, Katiyar (2011) Standardization of Fennel (Foeniculum vulgare), Its Oleoresin and Marketed Ayurvedic Dosage Forms, International Journal of Pharmaceutical Sciences and Drug Research; 3(3): 265-269.

[3]

Bahreini M, Torabi S, Omidi M, Vazan S (2013) Phytochemical study of Iranian Fennel (Foeniculum vulgare) Cell suspension culture extract, Journal of Biology and today’s world, volume 2, issue 11, page 498-500.

[4]

Bernath J., Nemeth E., Petheo F., Mihalik E., Kalman K., Franke R(1999).- Regularities of the essential oil accumulation in developing fruits of fennel (Foeniculum vulgare Mill.) and its histological background, J. Essent. Oil Res., 11, 431-438.

[5]

Billia, A. R, Flamini, G, Tagioli. V, I. Morelli, F. Vincieri. F (2002) GC–MS analysis of essential oil of some commercial Fennel teas, Volume 76, Issue 3, Pages 307–310.

[6]

Choi E. M., Hwang J. K. (2004) - Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare, Fitoterapia, 75 (6), 557-565;

[7]

Davis N. V.; (1990); “Gas Chromatographic Retention Indices of Monoterpenes and Sesquiterpenes on Methyl Silicone and Carbowax 20m Phases”; J. Chromatography.; 503, 1-24.

[8]

Hunault G, Desmarest P and Manoir JD (1989) XI Foeniculum vulgare Mill: Cell culture, regeneration and the production of anethole. In: Bajaj YPS (ed). Biotechnology in Agriculture and forestry, vol 7. Springer – Verlag, Berlin, Heidelberg; pp. 185-212.

[9]

Kapas Arpad, csabad. anras, gh .Dobre, Tanase, vass Erika (2011) kinetic of Essential oil separation from fennel by microwave assisted hyrodistillation (MWHD) U: P.B.SCI. BULL, SERIES, VOL. 73 issue4.

[10]

M. C. D´ıaz, M. S. P´erez-Coello, J. Esteban, and J. Sanz (2001) “Comparison of the volatile composition of wild fennel samples (Foeniculum vulgare Mill.) from Central Spain,” Journal of Agricultural

[11]

M. G. Miguel, C. Cruz, L. Faleiro et al (2010), “Foeniculum vulgare essential oils: chemical composition, antioxidant and antimicrobial activities,” Natural Product Communications, vol. 5, no. 2, pp. 319–328.

[12]

Najdoska, mence, bogdanov jane, zdravkovski zoran (2010) TLC and GC-MS anlyses of essential oil isolated from mecadonian foeniculi fructus, Macedonian pharmaceutical bulletin, 56 (1,2)29-36.

[13]

Rahimi R., Ardekani MR., (2013) Medicinal properties of Foeniculum vulgare Mill. traditional Iranian medicine and modern phytotherapy, Chin J Integr Med. 19(1): 73-9. doi: 10.1007/s11655-013-1327-0.

[14]

Sarkheil P., Omidi M., Peyghambari S. A. and Davazdahema. mi S, (2009) The effects of plant growth regulators and explants on callogenesis, regeneration and suspension culture in Foeniculum vulgare Mill. Iranian Journal of Medicinal and Aromatic Plants, Vol. 25, No. 3.

[15]

Shams Ardakani M. R., Abdi Kh., Jamshidi a. h., Haji Akhoundi a.(2005) the study of volatile oil of foeniculum vulgare miller, in their tissue culture and comparison with whole plant, journal of medical plants, 4(15): 0-0.

[16]

Singh gurdip, maurya sumitra, de lampasona M. P, catalan. c. (2006) Chemical constituents, antifungal and antioxidative potenyial of foeniculum vulgare oil and acetone extract, food control 17,745-752.

[17]

Trease and Evans (2009) Pharmacognosy, ISBN 10: 0702029335 ISBN 13: 9780702029332, Saunders Ltd, 16 editions. Publisher: Saunders Ltd., 2009.

[18]

Wagner H, Bladt S. (1996). A Thin Layer Chromatography Atlas, Edition 2nd, Springer-Verlag Heidelberg, Newyork,

[19]

Wagner H and Sabine B (1996) Plant drug analysis. 2th ed. Springer-Verlag; 150-1, 176.