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Evaluation of Cell Suspension Chemical Profile of Iranian Fennel by TLC and GC-MS
Current Issue
Volume 3, 2015
Issue 6 (December)
Pages: 74-77   |   Vol. 3, No. 6, December 2015   |   Follow on         
Paper in PDF Downloads: 90   Since Nov. 11, 2015 Views: 1506   Since Nov. 11, 2015
Authors
[1]
Maziar Bahreini, Razi Laboratory Complex, Science and Research Branch, Islamic Azad University, Tehran, Iran.
[2]
Fereydoon Bondarian, Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran.
[3]
Majid Gholibegian, Razi Laboratory Complex, Agronomy Lab, Science and Research Branch, Islamic Azad University, Tehran, Iran.
[4]
Kambiz Larijani, Department of Chemistry, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Abstract
Etheric oil of Apiacea family has notable importance in terms of health, and synthesis of pharmaceutical and cosmetics materials. Fennel (foeniculum vulgare) has been known as Mediterranean aroma plant and its volatile oil uses for anti-microbial, anti-fungal and favoring applications. The subject of this work was analysis of metabolite accumulation of calli which were suspended in darkness condition that isolated by n-Hexane, cell suspension has been made by ½ Murashige & Skoog (MS) with 2 mg l-1 2,4-D and 0.25 mg l-1 BAP as phytohorrmone combinations, semi quantitative analysis was carried out by TLC and Identification of extract constituents was done by GC-MS by calculation of Kovats indices using Homologous series of n-alkanes C8 -C19. In conclusion Decane was detected as major compound and other constituents were cyclic and acyclic hydrocarbons.
Keywords
Etheric Oil, Cell Suspension, Fennel
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