Ameliorating Effect of Ginkgo biloba and Moringa oleifera Against Equigan Induced Testicular Toxicity and Oxidative Stress in Male Rat
Background: Steroid hormones are illegally administered to food-producing animalsfor their muscle-building and growth-enhancing properties. Equigan or Equigan is one of the steroid hormones that have androgenic activity and cause infertility. The present study was designed to investigate the possible effect of Equigan on the structure and functions of rat testes and the role of Moringa leaf extract (MLE) and Ginkgo leaf extract (GLE) in treatments. Methods: A total of 48 Healthy male albino rats weighing 170-200 g and of 10-12 weeks age were divided into 6 groups (8 animals each). 1st Control group includes animals that injected intramuscularly with olive oil. 2nd and 3rd groups were rats received MLE (intragastrically, 200 mg/kg body weight) and GLE (intragastrically, 200 mg/kg body weight) respectively. 4th experimental group include animals that receive intramuscular injections of Equigan (5 mg/Kg body weight) for 12 weeks; while 5th and 6th groups where rats intramuscular injected with Equigan and treated with MLE and/or GLE at the same time respectively. Results: The obtained results indicate that testosterone has a significant decreased in control, Moringa, and Ginkgo groups when compared with its value in Equigan treated group. Also, testosterone in Equigan treated group has increased level when compared with co treated groups with MLE and GLE, total antioxidant activity represented in catalase, super oxide dismutase (SOD) and total thiolin testicular tissue showed a significant increase in co-treated groups with MLE and GLE compared with their values in Equigan treated group. On the other hand, the oxidative stress parameters such as malondialdhyde (MDA), and nitric oxide (NO) values showed a significant increase in Equigan treated group compared with their values co-treated groups with MLE and GLE. Conclusion: MLE and GLE has prophylactic roleon the biochemical and histopathological alteration in Equigan induced testicular toxicity in male rat.
Steroid Hormones, Equigan, Moringa Oleifera, Ginkgo Biloba, Testes, Oxidative Stress, Antioxidants, Rats
[1]
Aebi, H., 1984. Catalase in vitro.Methods Enzymol. 105, 121-126.
[2]
Ali, E., Tousson, E., and Abd El Daim, H., 2013. Effect of short and long term growth promoter boldenone undecylenate treatments on antioxidant enzyme activity and oxidative stress markers in rabbit muscles. Pharmacologia. 7, 576-581.
[3]
Arioli, F., Casati,A., Fidani,M., Silvestri, M. and Pompa, G., 2012. Prednisolone and prednisone neo-formation in bovine urine after sampling.Animal. 6, 1023-1029.
[4]
Aspimon, R., Bettini, A., Caner, A., Coughlan, J.A., and Levegrün, S., 1990. A study of the general characteristics of proton-antiproton collisions at √s=0.2 to 0.9 TeV. Nuclear Physics.335, 261- 287.
[5]
Atawodi, S.E., Atawodi, J.C., Idakwo, G.A., Pfundstein, B., Haubner, R., Wurtele, G., Spiegelhalder, B., Bartsch, H., and Owen, R., 2010. Evaluation of the Polyphenol Content and Antioxidant Properties of Methanol Extracts of the Leaves, Stem And Root Barks Of Moringa Oleifera, Lam. J. Med Food. 13(3), 710-716.
[6]
Bowers, L.D., and Wong, E.D., 1980. Kinetic serum creatinine assays II. A critical evaluation and review. Clin. Chem. 26, 555-561.
[7]
Chapman, C.S., 1998.Accountants in organisational networks. Accounting, Organizations and Society. 23, 737- 766.
[8]
Chattopadhyay, R.R., 2003.Possible mechanism of hepatoprotective activity of Azadirachta indicaleaf extract: Part II. Journal of Ethnopharmacology. 89, 217-219.
[9]
Christopher, S.F., Fendley, T.W., Dunn, R.T., and Queen, C.A., 1972. Improved Determination of Total Serum Lipids by the Sulfo-Phospho-Vanillin Reaction. Clinical Chemistry. 18 (7), 673-674.
[10]
DeLacerda, L., Kowarski, A., and Johanson, A.J., 1973. Integrated concentration and circadian variation of plasma testosterone in normal men. J. Clin Endocrinol Metab.37, 66-71.
[11]
El Mesallamy, H., Metwally, N., Soliman, M., Ahmed, K., and Abdel Moaty, M., 2011.The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats.Cancer Cell International. 11, 38.
[12]
Fossati, P., Prencipe, L., Aebi, H., and Berti, G., 1984. Use of 3,5-dichloro-2-hydroxybenzenesulfonic. Catalase in vitro.Methods Enzymol. 105, 121-126.
[13]
Furman, A., Henneberg, R., Hermann, P., Leonart, M., and Nascimento, A., 2012.Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients.Braz.J. Pharm. Sci. 48 (4),1984-8250.
[14]
Gabr, F., Abo El-Maaty, T., Amal, M., Aotifa, A.M., 2009. Effects of growth promoter boldenone undecylenate on weaned male lambs. Nature and Science. 7(3), 61-69.
[15]
Gavella, M., and Lipovac, V., 2013. Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa. Asian Journal of Andrology. 15, 375–381.
[16]
Grigorov, B., 2012. Reactive oxygen species and their relation to carcinogenesis.Trakia Journal of Sciences. 10(3), 83-92.
[17]
Hamza, A.A., 2010. Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats. Food Chem Toxicol. 48(1), 345-355.
[18]
Henry, R.F., 1974. Clinical chemistry principles and Techniques. 2nd ed. Harper, and Row, Hagerstown, M.D., {18} Schofeld, R.G., and Lowellen, C.S., 1964. Clin Chem. 10, 533.
[19]
Hernández, I., Alegre, L., Van Breusegem, F., and Munné-Bosch, S., 2009. How relevant are flavonoids as antioxidants in plants?Trends in Plant Science.14(3), 125–132.
[20]
Ji, L., Yin, X.X., Wu, Z.M., W, J.Y., Lu, Q., and Gao, Y.Y., 2009.Ginkgo biloba extract prevents glucose-induced accumulation of ECM in rat mesangial cells. Phytotherapy Reasearch. 4, 477–485.
[21]
Khan, A.A., Jilani, G., Akhtar, M.S., Saqlan, M., and Rasheed, M., 2009. Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production.J. AGRIC. BIOL. SCI.1, 48-58.
[22]
King, A.J., Hel, W., Cuevas, J.A., Ramiaramanana, D., and Grahaml, I.A., 2009. Potential of Jatropha curcas as a source of renewable oil and animal feed. Journal of Experimental Botany. 10, 2897-2905.
[23]
Koppers, A. J., De Iuliis, G. N., Finnie, J. M., McLaughlin, E. A., and Aitken, R. J., 2008. Significance of Mitochondrial Reactive Oxygen Species in the Generation of Oxidative Stress in Spermatozoa.J Clin Endocrinol Metab. 93, 3199–3207.
[24]
Lindstrom, F., and Diehl, H., 1960.Indicator for the titration of calcium plus magnesiumwith (ethylenedinitrilo)tetraacetate. Anal. Chem. 32, 1123-1127.
[25]
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.,1951.Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265-275.
[26]
Maneesh, M., and Jayalekshmi, H., 2006.Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction.Indian Journal of Clinical Biochemistry.21 (2), 80-89.
[27]
Maruna, R.F.L., 1958. Colorimetric determination of sodium in human serum and plasma. Clin. Chem. Acta. 2, 581.
[28]
Mesbah, L., Soraya, B., Narimane, S., and Jean, P.F., 2004. Protective effect of flavonoides against the toxicity of vinblastine cyclophosphamide and paracetamol by inhibition of lipid- peroxidation and increase of liver glutathione. Haema.7, 59-67.
[29]
Montgomery, H.A.C., and Dymock, J.F., 1961. The determination of nitrite in water. Analyst. 86, 414-416.
[30]
Nishikimi, M., Roa, N.A., and Yogi, K.,1972. Measurement of superoxide dismutase. Biochem. Biophys. Res. Common.46, 849-854.
[31]
Odell, W.D., Ross, G.T., and Rayford, P.L., 1981. Radioimmunoassay for luteinizing hormone in human plasma or serum: physiological studies. J. Clin Invest. 46, 248–255.
[32]
Owusu-Ansah, M., Achel, D. G., Adaboro, R. M., Asare, D. K., and Amoatey, H. M., 2011.Total Phenolic Content and Antioxidant Activity in Leaf Samples of Twelve Accessions of Moringa oleifera Lam. International Journal of Chemical and Analytical Science.2(10), 1226-1230.
[33]
Priya, V., Abiramasundari, P., Gayathri, Devi, S., and Jeyanthi, G.P., 2011. Antibacterilal activity of the leaves, bark, seed and flesh of Moringa oleifera. IJPSR. 2(8), 2045-2049.
[34]
Sahoo, T., Gaudio, D.D., German, J.R., Shinawi, M., Peters, U.S., Person, R.E., Garnica, A., Cheung, S.W.,and Beaudet, A. L., 2008. Prader-Willi phenotype caused by paternal deficiency for the HBII-85 C/D box small nucleolar RNA cluster. Brief Communication abstract. Nature Genetics . 40, 719-721.
[35]
Schmidt, H.A., Strimmer, K., Vingronl, M. and Haeseler, A.V., 2002.Maximum likelihood phylogenetic analysis using quartets and parallel computing. Oxford Journals. 18, 502-504.
[36]
Sedlak, J., and Lindsay, R.H., 1968. Estimation of total protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analytical biochemistry. 68, 90-92.
[37]
Shaker, E., Mahmoud, H., and Mnaa, S., 2010.Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food and Chemical Toxicology. 48, 803-806.
[38]
Shenton, M.E., Hamoda, H.M., Schneiderman, J.S., Bouix, S., Pasternak, O., Rathi, Y., Vu, M.A., Purohit, M.P., Helmer, k., Koerte, I., Lin, A.P., Westin, C.F., Kikinis, R., Stern, R.A., and Zafonte, R., 2012. A review of magnetic resonance imaging and diffusion tensor imaging findings in mild traumatic brain injury. Brain Imaging and Behavior. 2, 137-192.
[39]
Soma, L.R., Uboh, C.E., Guan, F., MC-Donnell, S., Pack, J., 2007.Pharmacokinetics of boldenone andstanozolol and the results of quantification ofanabolic and androgenic steroids in race horses andnonrace horses. J. Vet Pharmacol Therap.30, 101-108.
[40]
Sullivan, M.L., Martinez, C.M., Gennis, P., Gallagher, E.J., 1998.The cardiac toxicity of anabolic steroids.Prog Cardiovas Disc. 41, 1-15.
[41]
Tousson, E., Alm-Eldeen, A., and El-Moghazy, M., 2011. p53 and Bcl-2expression in response to boldenone induced liver cells injury.Toxicology and Industerial Health. 27(8), 711-718.
[42]
Tousson, E., El-Moghazy, M., Massoud, A., and Akel, A., 2012. Histopathological and immunohistochemical Changes in the testes of rabbits after injection with the growth promoter boldenone. Reproductive Sciences. 19(3), 253-259.
[43]
Tousson, E., 2013.Histopathological alterations after a growth promoter boldenone injection in rabbits.Toxicol Ind Health.10.1177/0748233713500821.
[44]
Tousson, E., Atteya, Z., El-Atrash, A., and Jeweely, O.I., 2014. Abrogation by Ginkgo Byloba Leaf Extract on Hepatic and Renal Toxicity Induced by Methotrexate in Rats.Journal of Cancer Research and Treatment.3, 44-51.
[45]
Trinder, P., 1951. Colorimetric determination of sodium inserum and plasma. Analyst. 76, 596.
[46]
Verheyden, K., Noppe, H., Zorn, H., Van Immerseel, F., Bussche, J.V., Wille, K., Bekaert, K., Janssen, C.R., De Brabander, H.F., and Vanhaecke, L., 2010.Endogenous boldenone-formation in cattle: Alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed. The Journal of Steroid Biochemistry and Molecular Biology. 119, 161-170.
[47]
Wang, Q., Kim, D., Dionysiou, D.D., Sorial, G.A., and Timberlake, D., 2004.Sources and remediation for mercury contamination in aquatic systemsa literature review. Environmental Pollution. 2, 323-336.
