This paper describes an easy hydrothermal method to prepare ZnO nanosheets with the hexagonal wurtzite structure using zinc nitrate and urea for the first time in our laboratory. There are 18 bacteria strain for easy growth in the media, among them 6-10 are fungal while rest are +ve and –ve Bacteria. ZnO show activity by reacting with them, this antibacterial activity of ZnO was successfully tested in our research Laboratory. The structural, compositions and morphology characterization of the as synthesized ZnO nanosheets was performed using X - ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS) and high resolution transmission scanning electron microscopy (HRTEM). The average thickness of ZnO nanosheets is found to be 12 nm, with the average diameter up to 90 nm. Antibiotics provide the main basis for the therapy of microbial (bacterial and fungal) infections. But with the emergence of drug resistance and other virulence factors especially formation of biofilm, the scenario has been changed. These antibiotics are becoming ineffective against bacterial and fungal infections. Therefore, we develop new approach of thermodynamic therapy in order to control the infection caused by MDR bacteria.

Nanostrucures, X-ray Diffraction, Antibacterial Activity, ZnO nanosheets