Degradation Study of Gentamicin by UV Spectroscopy
[1]
Safila Naveed, Faculty of Pharmacy, Jinnah University for Women, Karachi.
[2]
Nimra Waheed, Faculty of Pharmacy, Jinnah University for Women, Karachi.
[3]
Safeena Nazeer, Faculty of Pharmacy, Jinnah University for Women, Karachi.
[4]
Fatima Qamar, Faculty of Pharmacy, Jinnah University for Women, Karachi.
Gentamicin is an antibiotic from the class aminoglycoside having a broad spectrum activity. It is a bactericidal agent which is used to treat and prevent mycobacterial, enterococcal and severe Gram-negative bacterial infections. Degradation is a main problem in numerous unstable products. The forced degradation studies on drug substance involves photo Acid/base Stress testing, photo degradation, Temperature and or with humidity, Time, pH variation (low and high).In our recent research we study degradation parameters on gentamicin, for this we performed stress testing procedures on it. When gentamicin subjected to 0.1 N HCl and 0.1N NaOH, gentamicin showed increased availability. When gentamicin exposed to heat and U.V light (at wavelength max 243nm) for 30 minutes, deccreased availability was observed and in case of heating and it shown extremely decreased availability.
Gentamicin, Stress Testing, Degradation, U.V Spectrophotometer, Humidity
[1]
Joint Formulary Committee (editor) British National Formulary. 59th edn. London: British Medical Association and Royal Pharmaceutical Society of Great Britain; 2010.
[2]
Triggs E, Charles B. Pharmacokinetics and therapeutic drug monitoring of gentamicin in the elderly. Clin Pharmacokinet. 1999;37:331–41. [PubMed]
[3]
Robert Dagil, Charlotte O'shea, Anders Nykjær,Alexandre M. J. J. Bonvin, And Birthe B. Kragelund. Gentamicin Binds To The Megalin Receptor As A Competitive Inhibitor Using The Common Ligand Binding Motif Of Complement Type Repeats.Insight From The Nmr Structure Of The 10th Complement Type Repeat Domain Alone And In Complex With Gentamicin. J Biol Chem. Feb 8, 2013; 288(6): 4424–4435. Doi: 10.1074/Jbc.M112.434159.
[4]
Muhammad R Khan, Iram Badar, and Aisha Siddiquah Prevention of hepatorenal toxicity with Sonchus asper in gentamicin treated rats. BMC Complement Altern Med. 2011; 11: 113.doi: 10.1186/1472-6882-11-113.
[5]
Forge A., Schacht J. (2000) Aminoglycoside antibiotics. Audiol. Neurootol. 5, 3–22 [PubMed]
[6]
Yoshizawa S, Fourmy D, Puglisi JD. Structural origins of gentamicin antibiotic action. EMBO J.1998;17:6437–6448. [PMC free article] [PubMed]
[7]
Hilmer SN, McLachlan AJ, LeCouteur DG. Clinical pharmacology in the geriatric patient. Fundam Clin Pharmacol. 2007;21:217–30. [PubMed]
[8]
2. Forge A., Schacht J. (2000) Aminoglycoside antibiotics. Audiol. Neurootol. 5, 3–22 [PubMed]
[9]
5. Quiros Y., Vicente-Vicente L., Morales A. I., López-Novoa J. M., López-Hernández F. J. (2011) An integrative overview on the mechanisms underlying the renal tubular cytotoxicity of gentamicin. Toxicol. Sci. 119, 245–256 [PubMed]
[10]
Nina Isoherranen, Eran Lavy, and Stefan Soback :Pharmacokinetics of Gentamicin C1, C1a, and C2 in Beagles after a Single Intravenous Dose:Antimicrob Agents Chemother. Jun 2000; 44(6): 1443–1447.
[11]
Safila Naveed, Shabana Naz Shah, Fatima Qamar , Nimra Waheed, And Safeena Nazeersimple uv spectrophotometric assay of new formulation gentamycin. J App Pharm Vol. 6; Issue 4: 407-410; October, 2014
[12]
ICH. Stability testing of new drug substances and products Q1A (R2), International Conference on Harmonization, IFPMA, Geneva; 2003.
[13]
Safila naveed, nimra waheed and safeena nazeer (2014), DEGRADATION STUDIES OF AMPICILLIN IN API AND FORMULATIONS Journal of Applied Pharmacy Vol. 6; Issue 3: 314 -321; July, 2014
[14]
Kishore kumarhotha et al.(2013) Forced degradation studies: practical approach- overview of regulatory guidance and literature for the drug products and drug substances DOI: 10.7897/2230-8407.04517
[15]
Safila Naveed, Shafiq A, Khan M, Jamal M, Zafar H, et al. (2014) Degradation Study of Available Brands of Metformin in Karachi Using UV Spectrophotometer. JDiabetes Metabolism 5:328. doi:10.4172/2155-6156.1000328,IF 4.39 Index Copernicus Value: 6.17
[16]
Safila Naveed, Yusra Naseem, Siddiqua Samie, Sheerana Khan , Sana Siddiqui, Bushra and Salma Ramzan (2014) Degradation Study of Five Different Brands of Ciprofloxacin Using UV-Visible Spectrophotometer and Their Comparitive study (IRJP;5(3):189-190 http://www.irjponline.com/admin/php/uploads/2130_pdf.pdf
[17]
Safila Naveed*, Hina Qamar, Wardha Jawaid, Urooj Bokhari (2014) Effect of Acid, Base and Time on Different Brands of Glimepiride ,Open Access Library Journal, 1
[18]
SafilaNaveed, Nimra waheed and Safeena Nazeer (2014) degradation study of metronidazole in active and different formulation by uv spectroscopy J Bioequiv Availab 2014, 6:4, 124-127http://dx.doi.org/10.4172/jbb.1000191
