Trace Chromium in Human Urine Samples with Photometric Detection Method
[1]
Atanaska N. Bozhinova, Department of Chemistry and Biochemistry & Physics and Biophysics, University of Medicine-Pleven, Pleven, Bulgaria.
[2]
Maria G. Angelova, Department of Chemistry and Biochemistry & Physics and Biophysics, University of Medicine-Pleven, Pleven, Bulgaria.
Spectrophotometric method for determination of trace chromium in urine using Bis (salicylaldehyde) orthophenylenediamine in nonionic micellar media has been developed. In two variants of the pre-treatment (R1 and R2) of samples to be analyzed is applied for the study of the urine chromium in 64 people from 15 to 33 years of age. In R1, one of the reactants for the mineralization of the samples is perchloric acid. In the second method - R2 perchloric acid is replaced by hydrochloric acid. The methods gives a low limit of detection 0.02 μmol/l for R2 and 0.03 μmol/l for R1, good reproducibility with a relative standard deviation RSDR2 and RSDR1 of 1.33% and 1.37 % respectively, in the linear chromium concentration range 0.020 – 4.00 μmol/l. The analysis of 64 urine samples, taken from gave chromium concentration in the ranges 0.69 – 2.92 μmol/l and 0.64 – 3.34 μmol/l for R2- and R1- methods respectively. The RSD for 10 replicate analyses of a urine sample with an equally average chromium content of 2.23 μmol/l for R1 and R2–methods was 2.47% and 1.81% respectively. The reliability of the method was confirm determination of known chromium addition. Correlation coefficients between the added and found concentrations of chromium in urine were r R1 = 0.9790 and r R2 = 0.9907. A comparison of the results obtained by the method of standard straight and the method of the additives showed good results - rR1 = 0.9879 and rR2 = 0.9976, with good selectivity of methods. Analytical parameters of calibration and Urine chromium assay method R2 are compatible or better from the method R1. The analytical parameters of the two methods for the determination of total chromium in urine with spectrophotometric indication allowed to obtain comparable results to those of instrumental methods.
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