Determination of Serum Chromium and Application at Children with Primary Arterial Hypertension
[1]
Maria G. Angelova, Department of Chemistry and Biochemistry & Physics and Biophysics, University of Medicine-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria.
[2]
Atanaska N. Bozhinova, Department of Chemistry and Biochemistry & Physics and Biophysics, University of Medicine-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria.
[3]
Nadia Kolarova-Ianeva, Department of Pediatrics, University of Medicine-Pleven, 1 Kliment Ohridski Street, 5800 Pleven, Bulgaria.
Spectrophotometric method for determination of trace chromium using Bis (salicylaldehyde) orthophenylenediamine in nonionic micellar media applied for the study of the serum chromium in children with high blood pressure. The method gives a low limit of detection 0.02 µmol/l, good reproducibility with a relative standard deviation (RDS) of 2.20 to 4.30 % in the chromium concentration range 0.20 – 4.00 µmol/l. The analysis of 75 serum samples, taken from children with primary arterial hypertension and 25 healthy persons of 10-17 ages, gave chromium concentration in the ranges 0.40 – 1.20 µmol/l and 1.08 – 2.85 µmol/l, respectively. The RSD for 10 replicate analyses of a serum sample with an average chromium content of 0.82 µmol/l was 2.85%. The reliability of the method was verified by a determination based on known addition of chromium (r = 0.9879). A comparison of the method developed by the method of standard additions showed good matching results – r = 0.9766 and a good selectivity. Specifying the conditions of the analysis, except achieve the good analytical parameters allow also a reduction in the volume of blood serum for the analysis from 1 - 2 ml to 0.5 ml.
Serum Chromium Determination, Children with Primary Arterial Hypertension
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