Enumeration and Identification of Escherichia Coli, Salmonella and Vibrio Cholerae from Seafood in Utaewa Fishing Settlement, Ikot Abasi Local Government Area, Akwa Ibom State, Nigeria
[1]
Eze, V. C., Department of Microbiology, Michael Okpara University of Agriculture Umudike, P.M.B.7267, Umuahia, Abia State, Nigeria.
[2]
Edward, K. C., Department of Microbiology, Michael Okpara University of Agriculture Umudike, P.M.B.7267, Umuahia, Abia State, Nigeria.
[3]
Akwang, I. E., Department of Microbiology, Michael Okpara University of Agriculture Umudike, P.M.B.7267, Umuahia, Abia State, Nigeria.
The enumeration of Escherichia coli, Salmonella and Vibrio cholerae in seafood from Utaewa fishing settlement, Ikot Abasi Local Government Area, Akwa Ibom State was carried out. A total of 30 samples were collected. The media used were eosin methylene blue agar, Salmonella-Shigella agar, thiosulphate citrate bile-salt sucrose agar. The pour plate technique was used for the isolation of the microorganisms. The mean counts of Escherichia coli, Salmonella species and Vibrio cholerae for the fish gills, skin and intestine ranged from 6.28 ± 0.3Log10cfu/g to 6.56 ± 0.9Log10cfu/g; 5.90 ± 0.05Log10cfu/g to 6.38 ± 0.4Log10cfu/g and 6.60 ± 0.5Log10cfu/g to 6.62 ± 0.8Log10cfu/g respectively. The mean counts of Escherichia coli, Salmonella and Vibrio cholerae for the body and gut of the crab ranged from 6.08 ± 0.1Log10cfu/g to 6.49 ± 0.6 Log10cfu/g and 6.59 ± 0.3Log10cfu/g to 6.67 ± 0.5Log10cfu/g respectively. The mean counts of Escherichia coli, Salmonella and Vibrio cholerae for the periwinkle from locations 1, 2 and 3 ranged from 6.11 ± 0.2Log10cfu/g to 6.76 ± 0.7Log10cfu/g; 6.34 ± 0.1Log10cfu/g to 6.54 ± 0.3 Log10cfu/g and 0 ± 0.0Log10cfu/g to 6.40 ± 0.2Log10cfu/g respectively. This study shows that this seafood was grossly contaminated by these known pathogenic organisms and thus constitute potential public health hazard.
Isolation, Microbiological, Periwinkle, Microorganisms, Pollutants, Human Consumption, Crustaceans
[1]
Alexander, R., (1997). The invertebrates (2nd ed.) Cambridge University Press, London. pp. 259 - 287.
[2]
BoBelmann, F., Romanob, P., Fabritiusb, H., Raabeb D. and Epple, M. (2001).”The composition of the exoskeleton of two crustacean: The American lobster Homarus americanus and the edible crab Cancer pagunis”. Thermochimica Acta, 463(1-2): 65 – 68.
[3]
Rudloe, J. and Rudloe, A. (2009). Shrimps. The endless quest for pink. FT Press USA.
[4]
Brier, J. (1992). Emerging problem in seafood. Food Control; 3: 2 - 9.
[5]
Rice, R. (2004). Seafood - an essential part of 21st century eating patterns. Food Control; 8(2): 91 - 98.
[6]
Narin, N. and Nunes, M. (2007). Marine Animal and Plant Products. In: handbook of meat and seafood Quality, Nollet, L. and Boylston T. (Eds.). Blackwell Publishing, pp: 247.
[7]
Kris – Etherton, P., Hanis, W. and Appel, L. (2002). Fish consumption, fish oil, Omega-3 fatty acids and cardiovascular disease. 106: 2747 - 57.
[8]
Mozaffarian, D. and Rimm, E. (2006). Fish intake, Contaminants and Human Health: Evaluating the risk and the benefits. JAMA; 296: 1885 - 99.
[9]
Chen, H., Leey, L., Hwang, D. and Tibal, Y. (2010). Determination of histamine and bacterial isolation in Marun Fillets, Makaira nigrican implicated in a food-borne poisoning. Journal of Food Safety; 30: 699 -710.
[10]
Oken, E., Wright, R.O., KLeinman, K.P., Belliinger, D., Amarasinwardena, C.J., Hu, J., Rich-Edwards, J.W. and Gillman, M.W. (2005). Maternal fish consumption, hair mercury and infant cognition in a U.S. Cohort. Environmental Health Perspective; 1139(10): 1376 - 80
[11]
Najiah M., Wei, L., Shaharom, F. and Wee, W. (2008). Surveillance of bacterial species in fresh water and ornamental fish from aquarium shop. World Applied Science Journal; 3(6): 903 - 905.
[12]
Oyeleke, S. and Manga, S. (2008). Essentials of laboratory practical in Microbiology. Tobest Publisher, Minna, Nigeria, pp: 20 - 33.
[13]
Cheesbrough, M. (2005). District Laboratory Practice in Tropical Countries (2). Cambridge Press, United Kingdom, pp: 30 - 41.
[14]
Adeoye, A. (2007). Medical Laboratory Practice. (1st ed). FEMCO Publishers Limited, Lagos, Nigeria, pp: 153
[15]
Ochei, J.O. and A.A. Kolhatkar, 2007. Medical Laboratory Science: Theory and Practice, Tata McGraw-Hill Publishing Company Limited, New York, pp: 637 – 745.
[16]
Bergey’s Manual of Determinative Bacteriology, (1994). 9th ed., Holt, J.D. (Ed.), Williams Wilkins CO. Baltimore, pp. 783.
[17]
World Health Organization, (1996). Guidelines for water quality, (2nd ed). Geneva.
[18]
Ekanem, E. and Adejoke, G. (1995). Bacteriological study of claws and periwinkles. Food Microbiology; 12: 38 - 385.
[19]
U.S. Food and Drug Administration, (2013). Fresh and frozen seafood: selecting and serving it safely.
[20]
Gilbert, R., De Louvois, J. and Donovan, T. (1996). Microbiological guidelines for some ready-to-eat foods sampled at the point of sale. PHLS Microbiology Digest; 13: 41 - 43.
[21]
Ristori, C., Laria, S., Gelli, D. and Rivera, I. (2007). Pathogenic bacteria associated with oysters (Crassotrea brasiliana) and estuarine water along the South Coast of Brazil. International Journal of Environmental Health Resources; 17(4): 259 - 269.
[22]
Adebayo – Tayo, A., Odu, N., Michael, M. and Okonko, I. (2012). Multi-Drug Resistant (MDR). Organisms isolated from seafood in Uyo South – Southern Nigeria. Nature and Science; 10(3): 61-70.
[23]
Sciorino, J.A. and Ravikumar, R. (2009). Fishery Harbour Manual on the prevention of pollution. Bay of Bengal Programme Publishers, USA.