Association of Enteropathogenic Escherichia coli with or Without Diarrhea Among Children in Aba, Nigeria
[1]
Ome Kalu Achi, Department of Microbiology, Michael Okpara University of Agriculture, Umudike, PMB 7267, Umuahia, Abia State Nigeria.
[2]
Nonye Collect Mbajiaka, Department of Microbiology, Michael Okpara University of Agriculture, Umudike, PMB 7267, Umuahia, Abia State Nigeria.
The prevalence of enteropathogenic Escherichia coli (EPEC) and their antimicrobial resistance profiles were assessed in isolates from children with or without diarrhoea in Aba, Nigeria. Stool specimens were collected from two hundred infants and children under two years of age suspected with diarrhoeal disease from five hospitals in Aba. Specimens from healthy children (n½200) recruited from Maternal and Child Health Centres were analyzed for EPEC only. Colonies showing typical E. coli growth were tested using classical biochemical methods. The E. coli strains were further serotyped using enteropathogenic E. coli monovalent O: K antisera. Atypical EPEC was diagnosed in 19.5 % of the patients as compared to 6% in the healthy control group. The study revealed that the serotype 026k.60 B (6) had the highest significant effect (P< 0.05), indicating that it was most prevalent in Aba with rate of 48.7% followed by 0126 K 71 BC (16) and 0111 k58 B (4) with rates of 20.5% and 12.8%. A total of 17.9% of the isolated E. coli were untyped. The most susceptible age group infected by EPEC was 7-13 month followed by 14-20 month age group. Antibiotic susceptibility test showed that the enteropathogenic E. coli isolated was highly resistant to commonly used antibiotics Ampicillin, Septrin and Ampiclox with resistance rates of 89.7%, 71.8% and 64.1% respectively. More so Ciprofloxacin, Ceftriaxone and Tarivid showed high degree of sensitivity to the EPEC isolates with rates of 92.1%, 82.1% and 76.9% respectively. The maintenance of high personal hygiene by mothers and child minders with regards to the preparation and storage of infants and children foods is a major factor to avoid contamination of food by EPEC.
Enteropathogenic E. coli, Prevalence, Diarrhea, Antibiotic Resistance
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