Scanning Electron Microscopy of Fungi Isolated from Some Cake Samples
[1]
Sherif Mohamed El-Kadi, Department of Agricultural Microbiology, Faculty of Agriculture, Damietta University, Damietta, Egypt.
[2]
Husain Abdullah El-Fadaly, Department of Agricultural Microbiology, Faculty of Agriculture, Damietta University, Damietta, Egypt.
[3]
El-Said Metwaly El-Gayar, Department of Agricultural Microbiology, Faculty of Agriculture, Damietta University, Damietta, Egypt.
Thirty cake samples of twelve different companies were collected from Damietta and Dakahlia Governorates in September and October 2012. The highest value of total fungal counts was in C12 sample being 90.27×102cfu/g and the lowest value was in B27 sample being 0.27×102cfu/g. All samples were in production date except one sample (A4) was expired. The validity periods of most samples were between 3 and 6 months except one sample (C7) was one year. All samples were not in conformity with the Egyptian Organization for Standardization and Quality of cake (ES: 4037/2005). Fourteen fungal isolates were obtained from cake samples. Identification was based on the visual observation of fungal isolates grown on different cultivation media. The vegetative and reproductive strictness was observed by digital light microscope and scanning electron microscope (SEM). From these characteristics, fungal isolates were identified as Rhizopus stolonifer, Aspergillus niger, Aspergillus oryzae, Aspergillus flavus and Penicillium sp.
[1]
Ray, B. (2005). Fundamental Food Microbiology, 4thEd. CRC Press, Washington, USA.
[2]
EFSA, European Food Safety Authority (2007). Microbiological criteria and targets based on risk analysis. Opinion of the scientific panel on biological hazards on microbiological criteria and targets based on risk analysis. EFSA J. 462, 1-29.
[3]
Gorris, L. (2005). Food safety objectives: an integral part of food chain management. Food Control. 16, 801-809.
[4]
EU, European Union (2004). From Farm to Fork Safe Food for Europe´s Consumers. European Commission, Brussels.
[5]
Pundir, R. K., Jain, P. (2011). Qualitative and quantitative analysis of micro flora of Indian bakery products, Agricultural Technology, 7 (3), 751-762.
[6]
Hassan, R. A., El-Kadi, S. M., Mostafa, I. S. (2015). Effect of some organic acids on fungal growth and their toxins. International Journal of Advances in Biology (IJAB) 2 (1): 1-11.
[7]
El-Fadaly, H. A., El-Kadi, S. M., El-Gayar, E. A. (2016). Microbiological examination for some chocolate cake samples. Journal of Environmental Sciences, Mansoura Univ., 45 (1), 11-27.
[8]
Oxoid (2006). “Oxoid Manual”, 9thEd. Oxoid Limited, Wade Road, Basingstoke, Hampshire, England.
[9]
Benson, H. J. (2001). Microbiological Applications Lab Manual, 8thEd. The McGraw−Hill Companies, UK.
[10]
Anstead, G. M., Sutton, D. A., Thompson, E. H., Weitzman, I., Otto, R. A., Ahuja, S. K. (1999). Disseminated zygomycosis due to Rhizopusschipperae after heatstroke. J Clin Microbiol., 37, 2656-2662.
[11]
Frater, J. L., Hall, G. S., Procop, G. W. (2001). Histologic features of zygomycosis - Emphasis on perineural invasion and fungal morphology. Arch. Pathol. Lab. Med., 125, 375-378.
[12]
Buommin, N. R, Filippis, E. D., Lopez-Gresa, A., Manzo, M., Carella, E., Petrazzuolo, A., Tufano, M. M. A. (2009). Bioprospecting for antagonistic Penicilliumstrains as a resource of new antitumor compounds., World J. Microbiol., 24 (2), 185-95.
[13]
Bozzola, J. J., Russell, L. D. (1999). Electron Microscopy: Principles and Techniques for Biologists, 2ndEd. Jones and Bartlett Publishers, India.
[14]
Kuo, J. (2007). Electron Microscopy Methods and Protocols, 2ndEd. Humana Press Inc. Totowa, New Jersey.
[15]
Bancroft, J. D., Suvarna, S. K., Layton, C. (2013). Bancroft’s Theory and Practice of Histological Techniques, 7thEd., Elsevier, China.
[16]
Gumus, T., Dagliogli, O., Konyali, A. M. (2005). Microbiological quality of cream-cakes sold in Tekirdag Province. Tekirdag Agricultural Faculty, ISSN 1302-7050. 2, 215-220.
[17]
EOSQ, Egyptian Organization for Standardization and Quality (2005). Egyptian Standards for Requirements of Cake No: 4037.
[18]
Abellana, M., Sanchis, V., Ramos, A. J. (2001). Effect of water activity and temperature on growth of three Penicillium species and Aspergillusflavus on a sponge cake analogue. International Journal of Food Microbiology. 71, 151-157.
[19]
Guynot, M. E., Ramos, A. J., Sanchis, V., Marin, S. (2005). Study of benzoate, propionate, and sorbate salts as mould spoilage inhibitors on intermediate moisture bakery products of low pH (4.5-5.5). International Journal of Food Microbiology. 101, 161-168.
[20]
Deak, T. (2008). Handbook of Food Spoilage Yeasts, 2ndEd. CRC Taylor and Francis Group Boca Raton London New York, USA.
[21]
El-Fadaly, H., El-Kadi, S. M., Hamad, M. N. F. and Habib, A. (2015). Role of fungal enzymes in the biochemistry of Egyptian Ras cheese during ripening period. Open Access Library Journal, 2 (8), 1819-1832.
