[1]
Ubandoma Andefiki, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari Taraba State, Nigeria.
[2]
Imarenezor Edobor Peter Kenneth, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari Taraba State, Nigeria.
[3]
Brown Samuel Tamunoiyowuna Cockeye, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari Taraba State, Nigeria.
[4]
Tatah Silas Verwuye, Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari Taraba State, Nigeria.
[5]
Simon Shedrach, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari Taraba State, Nigeria.
[6]
Ebuara Francis Ushie, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University Wukari, Wukari Taraba State, Nigeria.
The antibacterial activities of ethanolic and aqueous extracts of leaves and stem bark extracts of Vitex doniana on Pseudomonas aeruginosa was investigated. The investigation of this traditionally used medicinal plant was necessary for two reasons; to ascertain its possible use as a source of chemotherapeutic drug(s) and to serve as a measure of the efficacy of herbal drugs. The plant was collected from Wukari in Taraba State, Nigeria and examined using standard methods. Bioactive substances including alkaloid, flavonoid, tannins, cardiac glycosides, resins, terpenes and steroids were found to be present in both leaves and stem bark extracts with pH of between 5 and 7. The stem bark extract was particularly more inhibitory to P. aeruginosa with minimum inhibitory concentration (MIC) of 4000 μg/ml while the leaf extracts had MIC greater than 4000 μg/ml. The Spectrophotometric absorbance also showed that different concentration of the extracts have varying degrees of activity on the organism. The plant was seen to be an effective antibacterial agent and therefore recommended for further studies and possible application in the treatment of such diseases as nosocomial infections in which P. aeruginosa is highly implicated.
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