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Isolation and Identification of Aspergillus Species from Wooden Fish (Keumamah)
Current Issue
Volume 5, 2018
Issue 3 (September)
Pages: 27-35   |   Vol. 5, No. 3, September 2018   |   Follow on         
Paper in PDF Downloads: 42   Since Jul. 2, 2018 Views: 1217   Since Jul. 2, 2018
Authors
[1]
Resmila Dewi, Faculty of Biology, University of Gadjah Mada, Yogyakarta, Indonesia.
Abstract
Wooden fish (keumamah) is a traditional food made from tuna (Euthynnus affinis) coated with wheat flour and be drained. During the storage and marketing, wooden fish may be contaminated by certain types of fungi, especially Aspergillus. The presence of fungi contaminants would reduce the wooden fish quality and dangerous for consumption. This study aims were to isolate and identify Aspergillus species in wooden fish. The study commenced with analysis of fungal population contaminating wooden fish samples from five major markets in Banda Aceh and isolated through serial dilution and spread plate method. The growths were identified using standard mycological techniques. Based on the analysis the fungal population on wooden fish was 0.5x102 –3.5x102 CFU/g. Colonies the grew were then taken to the purified to obtain pure cultures. The result of identification obtained four Aspergillus species contaminants on wooden fish namely A. flavus (JIK-1), A. fumigatus (JIK-2), A. ochracheus (JIK-3), and A. niger (JIK-4). The results showed that samples of wooden fish examined, all were contaminated with A. flavus (100%).
Keywords
Isolation, Identification, Fungi, Aspergillus, Wooden Fish, Keumamah
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