Identification and Antibiogram of Bacteria Associated with Urine of Diabetic Patients in Wukari, North East, Nigeria
[1]
Imarenezor Edobor Peter Kenneth, Tropical Diseases Unit, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria.
[2]
Brown Samuel Tamunoiyowuna Cockeye, Tropical Diseases Unit, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria.
Diabetic and bacteriuria was investigated in Wukari community, North East, Nigeria in order to determine the various bacteria associated with the disease and there antibiogram. A total of 80 individuals who are diabetic comprising of 47 (58.8%) males and 33 (41.2%) females had their urine samples examined using standard bacteriological techniques. The bacteria isolated include: Escherichia coli 27 (28.1%), Klebsiella pneumoniae 25 (26.0%), Pseudomonas aeruginosa 13 (13.5%), Proteus species 20 (20.8%) and Staphylococcus aureus 11 (11.5%) in the decreasing order of isolates. Antibiotic susceptibility testing of isolates showed Escherichia coli isolates was 100% sensitive to Ceftazidime, Gentamycin, Ofloxacin, and Augumentin, 58.1% sensitive to Nalidixic acid and Nitrofurantoin, and 73.1% sensitive to Ceftriaxone. It was 100% resistant to Tetracycline, Cotrimoxazole and Amoxicillin, 42.86% resistant to Nalidixic acid and Nitrofurantion and 28.57% resistant to Ceftriaxone. Staphylococcus aureus isolated from diabetic females was 100% sensitive to Ceftazidime, Gentamycin and Augumentin, 66.67% sensitive to Cloxacillin, and Chloramphenicol, 33.33% sensitive to Ofloxacin, Tetracycline, Cotrimoxazole, Amoxcillin, Streptomycin and Erythromycin. It was however, 100% resistant to Ceftriaxone. Klebsiella Pneumoniae isolated from diabetic males was 100% sensitive to Ceftriazone, Nalidixic Acid, Gentamycin, Augumentin, Tetracycline, and Ofloxacin, but 100% resistant to Cotrimoxazole and Amoxicillin. Proteus species and Pseudomonas aeruginosa isolates from male and female were 100% sensitive to Ceftazidime, Nalidixic acid, Gentamycin, Ofloxacin, Tetracycline, Ofloxacin, Cloxacillin, Streptomycin, Chloramphenicol and Augumentin, 100% resistant to Cotrimoxazole, Amoxicillin, Ceftazidime, Gentamycin, Cotrimoxazole and Erythromycin. Thus for all the bacterial isolates, Ceftazidime and Amoxicillin had the highest and lowest antimicrobial activities respectively. Results from this finding underscore the need for frequent urine examination for diabetic individuals and illustrates that bacteriuria is a major difficulty encountered in the management of diabetic. Therefore, the need to incorporate antibacterial therapy to the treatment, along with other public health interventions such as access to health education, health communication and appropriate case management.
Urine, Antibiogram, Bacteriuria, Wukari, Diabetic, North East, Nigeria
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