[1]
R. Sultana, Biotechnology Department, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[2]
A. K. M. L. Quader, Agronomy Department, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
[3]
Maksudul Haque, Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh.
[4]
S. Mazumder, Sher-e-Bangla Nagar Adorsha Mohila College, Dhaka, Bangladesh.
[5]
S. K. Paul, Agronomy Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.
The present study was undertaken to investigate the comparative regeneration potential of seedling explants of kenaf (Hibiscus cannabinus L.) at the Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, during the period from January, 2014 to June, 2014. An efficient regeneration protocol was developed for kenaf genotypes HC-2, HC-95 and HC-3 using root tip, cotyledon and hypocotyl as explants. For regeneration, Murashige and Skoog (MS) medium was used as culture medium and supplemented with different concentrations and combinations of NAA (α-napthaleneacetic acid) and BAP (6-Benzylaminopurine) as growth regulators. Different concentrations and combinations of hormones for callus induction formation NAA (0.0, 1.0, 1.0, 1.5, 2.0, 2.0 and 2.0 mg/L) and BAP (0.0, 1.0, 2.5, 5.0, 7.5, 10.0 and 15.0 mg/L) were used while, for shoot initiation BAP (0.0, 2.0, 4.0, 6.0, 8.0, 10.0 and 12.0 mg/L) were used. The HC-2 genotype had the highest callus induction (59.14) HC-95 genotype had the lowest value 37.70% for callus induction. The callus induction (71.41%) occurred the best on 2.0 mg/L NAA + 7.5 mg/L BAP combination. On the other hand, hypocotyl explant showed the highest callus induction (60.52%) followed by the other two explants (Root tip and cotyledon) which had lower callus induction (41.27%, 53.49%). The explant from the hypocotyl of genotype HC-2 showed the best performance on callus induction (91.33%) in 2.0 mg/L NAA +7.5 mg/L BAP combination within 7-10 days compared with genotype HC-95 and HC-3. The frequency of variation was found to be genotype dependent. Certain changes were found in regeneration of all the three genotypes, suggesting the existence of a mutation-sensitive part of the kenaf genome and possibility of improvement through somaclonal variation.
Hormonal Concentrations, Callus Induction, Explants, Kenaf (Hibiscus cannabinus L.)
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