Effects of Growth Conditions on Hydrophobicity in Marine Obligate Hydrocarbonoclastic Bacteria
[1]
Santina Santisi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy; Dep. of Biological and Environmental Sciences, Faculty of MM. FF. NN. University of Messina, Ph.D School in “Biology and Cellular Biotechnology” of University of Messina, Messina, Italy.
[2]
Maria Genovese, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
[3]
Francesca Crisafi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
[4]
Gabriella Gentile, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
[5]
Anna Volta, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy; Dep. of Industrial and Mechanical Engineering, Faculty of Engineering, University of Catania, Catania, Italy.
[6]
Martina Bonsignore, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
[7]
Simone Cappello, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
The aims of this work is study the influence of different growth conditions (different concentration of carbon source) on the variation of cellular hydrophobicity (capacity to adhere at polystyrene) of two obligate hydrocarbonoclastic bacteria, Alcanivorax borkumensis SK2 and Thalassolituus oleivorans MIL-1. The strains were inoculated, separately, in ONR7a mineral medium whit different concentration of sodium acetate. During all period of experimentation (10 days) measures of cellular abundance and cellular hydrophobicity (capacity to adhere at polystyrene) were carried out. Data obtained revealed as addition of carbon source and growth status are important factor in the regulation of adhesion to surface and as in these process Alcanivorax present an advantage respect Thalassolituus in all experimental condition. The understanding of capacity to adhesion of these bacteria for utilization of hydrophobic compounds is fundamental for their potential use in the mitigation of oil spills phenomenon and the importance of these preliminary data resides mainly in biotechnological applications, especially in reference to application of this bacteria in bioremediation strategies.
Adhesion, Alcanivorax, Bioremediation, Polystyrene, Thalassolituus
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