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Anatomy Study of Shoot Tips, Shoots, Flowers and Immature Fruits of Salvadora persica in Vitro
Current Issue
Volume 2, 2014
Issue 6 (December)
Pages: 150-153   |   Vol. 2, No. 6, December 2014   |   Follow on         
Paper in PDF Downloads: 15   Since Aug. 28, 2015 Views: 1328   Since Aug. 28, 2015
Authors
[1]
Abobkar A. M. Saad, Botany Department, Sebha University, Faculty of Science, Sebha, Libya.
[2]
Mansur Megna, Botany Department, Sebha University, Faculty of Science, Sebha, Libya.
Abstract
Shoot tips, Shoots, flowers and immature fruits were used as explants. They were inoculated on MS medium supplemented with different concentrations of some growth hormones. In case of using shoot tips on MS +NAA (0.1 mg/l) + BA (1mg/l), groups of parenchyma cells were formed meristematic centers and small buds were regenerated after 8 weeks of culture. When they cultured on MS+NAA (0.1 mg/l) + BA (1.5mg/l) small parenchyma cells contain prominent nuclei, tracheids were observed. Shoots on MS+Z (0.5mg/L) + AC (0.5mg/l) only small size of parenchyma cells surrounded by thin cell wall. When flowers were cultured on MS+ NAA (0.5mg/l) + BA (0.5mg/l) different sizes and shapes of parenchyma cells and tracheids were detected. For immature fruits on MS+2,4-D (0.5mg/l) small size of parenchyma cells contain thin cell wall and schlerenchyma cells were seen, On MS+NAA (0.5) + BA ( 1mg/l) only small parenchyma cells surrounded by thin cell wall were observed.
Keywords
Savadora perscia, Shoot Tips, Shoots, Flower, Immature Fruits, In Vitro
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